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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Myo1gtm1.2Mfk
targeted mutation 1.2, Matthew F Krummel
MGI:5581464
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Myo1gtm1.2Mfk/Myo1gtm1.2Mfk involves: C57BL/6 * FVB/N * SJL MGI:5581500
hm2
 		Myo1gtm1.2Mfk/Myo1gtm1.2Mfk Not Specified MGI:5581471
cx3
 		Myo1gtm1.2Mfk/Myo1gtm1.2Mfk
Tg(TcraTcrb)1100Mjb/0 		involves: C57BL/6 * FVB/N * SJL MGI:5581501


Genotype
MGI:5581500
hm1
Allelic
Composition		 Myo1gtm1.2Mfk/Myo1gtm1.2Mfk
Genetic
Background		 involves: C57BL/6 * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myo1gtm1.2Mfk mutation (0 available); any Myo1g mutation (52 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell physiology ( J:212451 )
• in a 3D matrix, T cells arrest more often than wild-type cells
• when motile, T cells migrate twice as fast as wild-type cells
• arrested T cells exhibit defective spontaneous reinitiation of migration compared with wild-type cells
• following wash-out of blebbistatin, T cells take 3 times as long to reinitiate polarization compared with wild-type cells
• in lymph nodes, T cells exhibit faster movement with reduced turning angle compared with wild-type cells
• however, T cells exhibit normal arrest rates in a 2D matrix, homing to lymph nodes in vivo and antigen recognition

hematopoietic system
abnormal T cell physiology ( J:212451 )
• in a 3D matrix, T cells arrest more often than wild-type cells
• when motile, T cells migrate twice as fast as wild-type cells
• arrested T cells exhibit defective spontaneous reinitiation of migration compared with wild-type cells
• following wash-out of blebbistatin, T cells take 3 times as long to reinitiate polarization compared with wild-type cells
• in lymph nodes, T cells exhibit faster movement with reduced turning angle compared with wild-type cells
• however, T cells exhibit normal arrest rates in a 2D matrix, homing to lymph nodes in vivo and antigen recognition




Genotype
MGI:5581471
hm2
Allelic
Composition		 Myo1gtm1.2Mfk/Myo1gtm1.2Mfk
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myo1gtm1.2Mfk mutation (0 available); any Myo1g mutation (52 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:209304 )
N
• B cells exhibit normal activation and proliferation
• mice exhibit normal spleen B cell subsets
decreased B cell number ( J:209304 )
• in lymph nodes and spleen
increased B cell number ( J:209304 )
• in peripheral blood
abnormal B cell physiology ( J:209304 )
• in vitro, B cell exhibit reduced anti-CD44- or anti-LFA-1-induced spreading with failure to develop long membrane projections and reduced adhesion compared with wild-type cells
• in vivo, B cells exhibit slow migration and defective homing compared with wild-type cells
• peritoneal B cells ingest more FITC-bacteria than wild-type cells
decreased tumor necrosis factor secretion ( J:209304 )
• from cultured spleen cells

hematopoietic system
decreased B cell number ( J:209304 )
• in lymph nodes and spleen
increased B cell number ( J:209304 )
• in peripheral blood
abnormal B cell physiology ( J:209304 )
• in vitro, B cell exhibit reduced anti-CD44- or anti-LFA-1-induced spreading with failure to develop long membrane projections and reduced adhesion compared with wild-type cells
• in vivo, B cells exhibit slow migration and defective homing compared with wild-type cells
• peritoneal B cells ingest more FITC-bacteria than wild-type cells




Genotype
MGI:5581501
cx3
Allelic
Composition		 Myo1gtm1.2Mfk/Myo1gtm1.2Mfk
Tg(TcraTcrb)1100Mjb/0
Genetic
Background		 involves: C57BL/6 * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myo1gtm1.2Mfk mutation (0 available); any Myo1g mutation (52 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell physiology ( J:212451 )
• T cells exhibit decreased antigen detection efficiency compared with wild-type cells

hematopoietic system
abnormal T cell physiology ( J:212451 )
• T cells exhibit decreased antigen detection efficiency compared with wild-type cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory