Phenotypes associated with this allele

• Allele Symbol: Ikzf1^tm2.1Smale
• Allele Name: targeted mutation 2.1, Stephen T Smale
• Allele ID: MGI:5563076
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ikzf1^tm2.1Smale/Ikzf1^tm2.1Smale	B6.Cg-Ikzf1^tm2.1Smale	MGI:5563080
ht2	Ikzf1^tm2Kge/Ikzf1^tm2.1Smale	involves: 129S4/SvJae * C57BL/6	MGI:5563082

Genotype
MGI:5563080

hm1

• Allelic Composition: Ikzf1^tm2.1Smale/Ikzf1^tm2.1Smale
• Genetic Background: B6.Cg-Ikzf1^tm2.1Smale

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:208211 )

• associated with development of lymphoma

immune system

immune system phenotype ( J:208211 )

N • mice exhibit normal numbers of pro-B, pre-BI cells and DN4 cells

thymus cortex hypoplasia ( J:208211 )

thymus hypoplasia ( J:208211 )

• slight

abnormal lymphopoiesis ( J:208211 )

• absence of B and T lineage cells in the fetus

• absent lymphoid tissue-inducer cells from fetal intestinal mesentery

decreased immature B cell number ( J:208211 )

• to a lesser extent than in Ikzf1^tm1.1Smale

decreased pre-pro B cell number ( J:208211 )

abnormal T cell differentiation ( J:208211 )

• reduced ratio of double-positive to single-positive thymocytes

decreased double-negative T cell number ( J:208211 )

decreased DN2 thymocyte number ( J:208211 )

decreased DN3 thymocyte number ( J:208211 )

decreased double-positive T cell number ( J:208211 )

decreased plasmacytoid dendritic cell number ( J:208211 )

• absent

decreased B cell number ( J:208211 )

• smaller number of conventional B cells in the bone marrow and spleen

decreased mature B cell number ( J:208211 )

• recirculating; to a lesser extent than in Ikzf1^tm1.1Smale

increased pre-B cell number ( J:208211 )

• of large pre-BII cells

absent NK cells ( J:208211 )

increased gamma-delta T cell number ( J:208211 )

absent Peyer's patches ( J:208211 )

• in adult mice

abnormal peripheral lymph node morphology ( J:208211 )

• absence of inguinal and lumbar lymph nodes in adult mice

• however, nasal-associated lymphoid tissue is normal

absent inguinal lymph nodes ( J:208211 )

• in adult mice

neoplasm

increased lymphoma incidence ( J:208211 )

• highly invasive and metastatic thymic lymphoma in some mice

growth/size/body

decreased body size ( J:208211 )

• some mice are runted

hematopoietic system

thymus cortex hypoplasia ( J:208211 )

thymus hypoplasia ( J:208211 )

• slight

abnormal definitive hematopoiesis ( J:208211 )

• low abundance of B220+CD43+CD24-BP1- cells (precursors to NK cells and plasmacytoid dendritic cells)

abnormal lymphopoiesis ( J:208211 )

• absence of B and T lineage cells in the fetus

• absent lymphoid tissue-inducer cells from fetal intestinal mesentery

decreased immature B cell number ( J:208211 )

• to a lesser extent than in Ikzf1^tm1.1Smale

decreased pre-pro B cell number ( J:208211 )

abnormal T cell differentiation ( J:208211 )

• reduced ratio of double-positive to single-positive thymocytes

decreased double-negative T cell number ( J:208211 )

decreased DN2 thymocyte number ( J:208211 )

decreased DN3 thymocyte number ( J:208211 )

decreased double-positive T cell number ( J:208211 )

decreased plasmacytoid dendritic cell number ( J:208211 )

• absent

decreased B cell number ( J:208211 )

• smaller number of conventional B cells in the bone marrow and spleen

decreased mature B cell number ( J:208211 )

• recirculating; to a lesser extent than in Ikzf1^tm1.1Smale

increased pre-B cell number ( J:208211 )

• of large pre-BII cells

absent NK cells ( J:208211 )

increased gamma-delta T cell number ( J:208211 )

endocrine/exocrine glands

thymus cortex hypoplasia ( J:208211 )

thymus hypoplasia ( J:208211 )

• slight

decreased DN2 thymocyte number ( J:208211 )

decreased DN3 thymocyte number ( J:208211 )

Genotype
MGI:5563082

ht2

• Allelic Composition: Ikzf1^tm2Kge/Ikzf1^tm2.1Smale
• Genetic Background: involves: 129S4/SvJae * C57BL/6

mortality/aging

lethality, complete penetrance ( J:208211 )

• no mice are produced presumably because of embryonic lethality of Ikzf1^tm2Kge on a C57BL/6 background