Phenotypes associated with this allele

• Allele Symbol: Atf4^tm1.1Cmad
• Allele Name: targeted mutation 1.1, Christopher Adams
• Allele ID: MGI:5550363
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Atf4^tm1.1Cmad/Atf4^tm1.1Cmad Tg(Myh11-icre/ERT2)1Soff/?	D2.Cg-Atf4^tm1.1Cmad Tg(Myh11-icre/ERT2)1Soff	MGI:5812899
cn2	Atf4^tm1.1Cmad/Atf4^tm1.1Cmad Tg(Tnnt2-cre)5Blh/0	involves: C57BL/6 * DBA/2	MGI:8401672
cn3	Atf4^tm1.1Cmad/Atf4^tm1.1Cmad Tg(Ckmm-cre)5Khn/0	involves: C57BL/6 * FVB	MGI:5550389
cn4	Atf4^tm1.1Cmad/Atf4^tm1.1Cmad Tg(myl7.L-cre)1118Tmhn/0	involves: C57BL/6 * MF1	MGI:8401249

Genotype
MGI:5812899

cn1

• Allelic Composition: Atf4^tm1.1Cmad/Atf4^tm1.1Cmad; Tg(Myh11-icre/ERT2)1Soff/?
• Genetic Background: D2.Cg-Atf4^tm1.1Cmad Tg(Myh11-icre/ERT2)1Soff

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal aorta morphology ( J:237331 )

♂ • following nephrectomy (to induce chronic kidney disease) and administration of tamoxifen, mice developed smaller calcified medial lesions than nephrectomized controls and similar to sham-operated controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:237331 )

♂N • following nephrectomy (to induce chronic kidney disease) and administration of tamoxifen, levels of serum creatinine, phosphorus, calcium, cholesterol and triglyceride are similar to levels in nephrectomized wild-type mice

abnormal calcium ion homeostasis ( J:237331 )

♂ • following nephrectomy (to induce chronic kidney disease) and administration of tamoxifen, aortic calcium content was lower than nephrectomized controls, but higher than sham-operated controls

Genotype
MGI:8401672

cn2

• Allelic Composition: Atf4^tm1.1Cmad/Atf4^tm1.1Cmad; Tg(Tnnt2-cre)5Blh/0
• Genetic Background: involves: C57BL/6 * DBA/2

muscle

thin ventricle myocardium compact layer ( J:379595 )

cardiovascular system

abnormal heart morphology ( J:379595 )

• cardiac defects identical to mice with conditional loss driven by Tg(myl7.L-cre)1118Tmhn

thin ventricle myocardium compact layer ( J:379595 )

Genotype
MGI:5550389

cn3

• Allelic Composition: Atf4^tm1.1Cmad/Atf4^tm1.1Cmad; Tg(Ckmm-cre)5Khn/0
• Genetic Background: involves: C57BL/6 * FVB

muscle

muscle phenotype ( J:206503 )

N • mice exhibit normal skeletal muscle development

decreased susceptibility to induced muscular atrophy ( J:206503 )

• following fasting or 3 days of immobilization

• however, muscular atrophy following 7 days of immobilization is normal

Genotype
MGI:8401249

cn4

• Allelic Composition: Atf4^tm1.1Cmad/Atf4^tm1.1Cmad; Tg(myl7.L-cre)1118Tmhn/0
• Genetic Background: involves: C57BL/6 * MF1

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:379595 )

• die between E15.5 and E17.5

cardiovascular system

abnormal heart morphology ( J:379595 )

• at E12.5

thin ventricle myocardium compact layer ( J:379595 )

• in both ventricles at E12.5

decreased fetal cardiomyocyte proliferation ( J:379595 )

• reduced proliferation of embryonic cardiomyocytes at E11.5

• however, apoptosis is similar to wild-type controls

muscle

thin ventricle myocardium compact layer ( J:379595 )

• in both ventricles at E12.5

decreased fetal cardiomyocyte proliferation ( J:379595 )

• reduced proliferation of embryonic cardiomyocytes at E11.5

• however, apoptosis is similar to wild-type controls

cellular

decreased fetal cardiomyocyte proliferation ( J:379595 )

• reduced proliferation of embryonic cardiomyocytes at E11.5

• however, apoptosis is similar to wild-type controls