Phenotypes associated with this allele

• Allele Symbol: Trp53^tm1.1Manf
• Allele Name: targeted mutation 1.1, James J Manfredi
• Allele ID: MGI:5550216
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trp53^tm1.1Manf/Trp53^tm1.1Manf	involves: C57BL/6J	MGI:6383837
hm2	Trp53^tm1.1Manf/Trp53^tm1.1Manf	Not Specified	MGI:5551468

Genotype
MGI:6383837

hm1

• Allelic Composition: Trp53^tm1.1Manf/Trp53^tm1.1Manf
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:201153 )

• mice die by 2 weeks of age

nervous system

decreased brain size ( J:201153 )

abnormal cerebellar cortex morphology ( J:201153 )

abnormal cerebellar foliation ( J:201153 )

• shallower

• abnormal pattern

abnormal cerebellar Purkinje cell layer ( J:201153 )

• Purkinje cells fail to form a uniform monolayer

thin cerebellar granule layer ( J:201153 )

• both internal and external layers are thinner with reduced cellularity

absent cerebellum vermis ( J:201153 )

• virtually absent

behavior/neurological

ataxia ( J:201153 )

growth/size/body

enlarged heart ( J:201153 )

postnatal growth retardation ( J:201153 )

• appear normal at 1 day after birth

• markedly reduced in size at 10 days of age

skeleton

abnormal tibia morphology ( J:201153 )

• reduced in size

pigmentation

abnormal digit pigmentation ( J:201153 )

cardiovascular system

enlarged heart ( J:201153 )

cellular

decreased hematopoietic stem cell proliferation ( J:201153 )

• absolute numbers of hematopoietic stem cells are reduced

endocrine/exocrine glands

abnormal thymus morphology ( J:201153 )

• less cellular, particularly in the cortex

small thymus ( J:201153 )

• reduced in size

hematopoietic system

decreased hematopoietic stem cell proliferation ( J:201153 )

• absolute numbers of hematopoietic stem cells are reduced

abnormal thymus morphology ( J:201153 )

• less cellular, particularly in the cortex

small thymus ( J:201153 )

• reduced in size

abnormal definitive hematopoiesis ( J:201153 )

extramedullary hematopoiesis ( J:201153 )

• normal at E18.5 but absent at 10-12 days of age

anemia ( J:201153 )

• severe anemia at 10 days of age

decreased bone marrow cell number ( J:201153 )

• cellularity begins declining at 1 day of age

• virtually aplastic at 10-12 days of age

decreased neutrophil cell number ( J:201153 )

thrombocytopenia ( J:201153 )

abnormal spleen morphology ( J:201153 )

small spleen ( J:201153 )

• reduced spleen size and cellularity

• sparse extramedulary hematopoiesis

abnormal spleen white pulp morphology ( J:201153 )

• less cellularity

immune system

abnormal thymus morphology ( J:201153 )

• less cellular, particularly in the cortex

small thymus ( J:201153 )

• reduced in size

decreased neutrophil cell number ( J:201153 )

abnormal spleen morphology ( J:201153 )

small spleen ( J:201153 )

• reduced spleen size and cellularity

• sparse extramedulary hematopoiesis

abnormal spleen white pulp morphology ( J:201153 )

• less cellularity

abnormal abdominal lymph node morphology ( J:201153 )

• gut associated lymphoid tissue is not apparent or shows very reduced cellularity

integument

abnormal digit pigmentation ( J:201153 )

limbs/digits/tail

abnormal limb morphology ( J:201153 )

abnormal digit pigmentation ( J:201153 )

abnormal tibia morphology ( J:201153 )

• reduced in size

abnormal tail tip morphology ( J:201153 )

• abnormal tail tip

kinked tail ( J:201153 )

Genotype
MGI:5551468

hm2

• Allelic Composition: Trp53^tm1.1Manf/Trp53^tm1.1Manf
• Genetic Background: Not Specified

mortality/aging

postnatal lethality, complete penetrance ( J:201153 )

• mice die by 2 weeks of age

growth/size/body

enlarged heart ( J:201153 )

postnatal growth retardation ( J:201153 )

• appear normal at 1 day after birth

• markedly reduced in size at 10 days of age

limbs/digits/tail

abnormal limb morphology ( J:201153 )

abnormal digit pigmentation ( J:201153 )

abnormal tibia morphology ( J:201153 )

• reduced in size

abnormal tail tip morphology ( J:201153 )

• abnormal tail tip

kinked tail ( J:201153 )

behavior/neurological

ataxia ( J:201153 )

cardiovascular system

enlarged heart ( J:201153 )

hematopoietic system

decreased hematopoietic stem cell proliferation ( J:201153 )

• absolute numbers of hematopoietic stem cells are reduced

abnormal thymus morphology ( J:201153 )

• less cellular, particularly in the cortex

small thymus ( J:201153 )

• reduced in size

abnormal definitive hematopoiesis ( J:201153 )

extramedullary hematopoiesis ( J:201153 )

• normal at E18.5 but absent at 10-12 days of age

anemia ( J:201153 )

• severe anemia at 10 days of age

decreased bone marrow cell number ( J:201153 )

• cellularity begins declining at 1 day of age

• virtually aplastic at 10-12 days of age

decreased neutrophil cell number ( J:201153 )

thrombocytopenia ( J:201153 )

abnormal spleen morphology ( J:201153 )

small spleen ( J:201153 )

• reduced spleen size and cellularity

• sparse extramedulary hematopoiesis

abnormal spleen white pulp morphology ( J:201153 )

• less cellularity

immune system

abnormal thymus morphology ( J:201153 )

• less cellular, particularly in the cortex

small thymus ( J:201153 )

• reduced in size

decreased neutrophil cell number ( J:201153 )

abnormal spleen morphology ( J:201153 )

small spleen ( J:201153 )

• reduced spleen size and cellularity

• sparse extramedulary hematopoiesis

abnormal spleen white pulp morphology ( J:201153 )

• less cellularity

abnormal abdominal lymph node morphology ( J:201153 )

• gut associated lymphoid tissue is not apparent or shows very reduced cellularity

nervous system

decreased brain size ( J:201153 )

abnormal cerebellar cortex morphology ( J:201153 )

abnormal cerebellar foliation ( J:201153 )

• shallower

• abnormal pattern

abnormal cerebellar Purkinje cell layer ( J:201153 )

• Purkinje cells fail to form a uniform monolayer

thin cerebellar granule layer ( J:201153 )

• both internal and external layers are thinner with reduced cellularity

absent cerebellum vermis ( J:201153 )

• virtually absent

skeleton

abnormal tibia morphology ( J:201153 )

• reduced in size

pigmentation

abnormal digit pigmentation ( J:201153 )

cellular

decreased hematopoietic stem cell proliferation ( J:201153 )

• absolute numbers of hematopoietic stem cells are reduced

endocrine/exocrine glands

abnormal thymus morphology ( J:201153 )

• less cellular, particularly in the cortex

small thymus ( J:201153 )

• reduced in size

integument

abnormal digit pigmentation ( J:201153 )