Trp53^tm1.1Manf
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1.1Manf
• Name: transformation related protein 53; targeted mutation 1.1, James J Manfredi
• MGI ID: MGI:5550216
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1.1Manf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201153
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An FRT site and a loxP site were inserted in intron 10 and a neomycin resistance cassette followed by a second loxP site was inserted following exon 11. Finally, a second exon 11 with a stop codon in codon 367 (AAG to TAG change) was inserted after the second loxP site. Excision of the first copy of exon 11 by cre expression results in expression from the second copy of exon 11 which contains a premature stop codon leading to a truncated protein lacking the final 24 amino acids. (J:201153)

Expression

In Mice Carrying this Mutation:	130 assay results
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

References

• Original: J:201153 Hamard PJ, et al., The C terminus of p53 regulates gene expression by multiple mechanisms in a target- and tissue-specific manner in vivo. Genes Dev. 2013 Sep 1;27(17):1868-85
• All: 3 reference(s)