Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm2.1(cre/Esr1*)Ims mutation
(0 available);
any
Runx1 mutation
(35 available)
|
|
|
mortality/aging
|
|
• all embryos die around E12.5
|
hematopoietic system
|
|
• embryos exhibit severe liver anemia
|
cardiovascular system
|
|
• embryos display a severe hemorrhagic phenotype
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm1Medv mutation
(0 available);
any
Runx1 mutation
(35 available)
Runx1tm2.1(cre/Esr1*)Ims mutation
(0 available);
any
Runx1 mutation
(35 available)
|
|
|
mortality/aging
|
|
• maternal injection of 200-250 mg of 4-hydroxytamoxifen (4-OHT) on E6.5 or E7.5 rescues prenatal lethality, but pups, although born apparently normal, are unable to feed and die within 1 day of birth
|
|
|
• all embryos die around E12.5
• maternal injection of 5-6 mg of 4-hydroxytamoxifen (4-OHT) on E6.5 or E7.5 to reactivate the Runx1lacZ allele rescues 90-100% of the E12.5 lethality; similarly, a low dose of tamoxifen (2 mg) at E7.5 also results in robust (80-100%) rescue of lethality assayed at E16.5 or P0
• reactivation of the Runx1lacZ allele with maternal tamoxifen treatment at E8.0, 8.5, 9.0, or 9.5 does not rescue the hemorrhagic or liver anemia phenotypes; pregastrulation tamoxifen injection at E5.5 does not result in embryo rescue
|
hematopoietic system
|
N |
• with maternal injection of high-dose tamoxifen on E7.5 to induce Runx1 reactivation, restoration of definitive hematopoiesis is observed by E16.5; FACS analyses of blood cell populations are similar to control heterozygous cells
|
|
|
• tamoxifen treatment at times after E7.5 results in no rescue of definitive hematopoietic progenitor cells; only a few progenitors can be observed with tamoxifen treatment at E8.5 with none found with later treatment timepoints
|
|
|
• embryos exhibit severe liver anemia
|
cardiovascular system
|
|
• embryos display a severe hemorrhagic phenotype
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(EYFP)Cos mutation
(11 available);
any
Gt(ROSA)26Sor mutation
(942 available)
Runx1tm1Medv mutation
(0 available);
any
Runx1 mutation
(35 available)
Runx1tm2.1(cre/Esr1*)Ims mutation
(0 available);
any
Runx1 mutation
(35 available)
|
|
|
hematopoietic system
|
N |
• with maternal injection of high-dose tamoxifen on E7.5 to induce Runx1 reactivation, trasnsplanted, hematopoietic stem cells were isolated and injected into irradiated recipients for a long-term competitive repopulation assay; at E14.5 and E16.5, no signficant differences in reconstitution levels of hematopoietic cells is observed relative to controls
• secondary transplantation of yolk sac-derived bone marrow cells from the primary recipients 12 months following primary transplantation resulted in long-term engraftment
|
|
|
• exposure of embryos to tamoxifen at E10.5 fails to result in observation of CD45+ definitive hematopoietic cells in the aorta-gonad-mesonephros (AGM) region (no rescue of definitive hematopoiesis), whereas after exposure to tamoxifen at E7.5, a large population of CD45-positive cells are detected
|
Analysis Tools