Phenotypes associated with this allele

• Allele Symbol: Ncr1^{tm1.1(icre)Viv}
• Allele Name: targeted mutation 1.1, Eric Vivier
• Allele ID: MGI:5308410
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ncr1^tm1.1(icre)Viv/Ncr1^tm1.1(icre)Viv	involves: C57BL/6	MGI:5309017
ht2	Ncr1^tm1.1(icre)Viv/Ncr1^+	involves: C57BL/6	MGI:5308422
cn3	Sppl3^tm1Itl/Sppl3^tm1Itl Ncr1^tm1.1(icre)Viv/Ncr1^+	involves: 129S/SvEv * C57BL/6	MGI:6515783
cn4	Sppl3^tm1Itl/Sppl3^em1Pomz Ncr1^tm1.1(icre)Viv/Ncr1^+	involves: 129S/SvEv * C57BL/6 * C57BL/6J	MGI:6515792
cn5	Rfx7^tm1.1Ggaur/Rfx7^tm1.1Ggaur Ncr1^tm1.1(icre)Viv/Ncr1^+	involves: C57BL/6	MGI:6513975

Genotype
MGI:5309017

hm1

• Allelic Composition: Ncr1^{tm1.1(icre)Viv}/Ncr1^{tm1.1(icre)Viv}
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:179728 )

• more resistant to MCMV infection

decreased susceptibility to Orthomyxoviridae infection induced morbidity/mortality ( J:179728 )

• more resistant to H1N1PR8 infection

immune system

abnormal NK cell physiology ( J:179728 )

• cultured cells are hyperresponsive to YAC-1 target cells and NK1.1 stimulation

decreased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:179728 )

• more resistant to MCMV infection

decreased susceptibility to Orthomyxoviridae infection induced morbidity/mortality ( J:179728 )

• more resistant to H1N1PR8 infection

hematopoietic system

abnormal NK cell physiology ( J:179728 )

• cultured cells are hyperresponsive to YAC-1 target cells and NK1.1 stimulation

Genotype
MGI:5308422

ht2

• Allelic Composition: Ncr1^{tm1.1(icre)Viv}/Ncr1⁺
• Genetic Background: involves: C57BL/6

hematopoietic system

hematopoietic system phenotype ( J:180265 )

N • mice show no significant variation in numbers fo lymphoid and myeloid subsets compared with wild-type; NK cell counts and in vitro effector function are normal

normal phenotype

no abnormal phenotype detected ( J:180265 )

• mice are born at normal Mendelian frequencies, are viable and fertile

Genotype
MGI:6515783

cn3

• Allelic Composition: Sppl3^tm1Itl/Sppl3^tm1Itl; Ncr1^{tm1.1(icre)Viv}/Ncr1⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

immune system

abnormal NK cell differentiation ( J:280182 )

• mice show subtle changes in the expression of NK cell surface receptors and a defect in NK cell maturation with a 5-fold reduction of CD27+CD11b+ NK cells in spleen and a 2.7-fold loss of CD27+CD11b+ NK cells in the bone marrow

increased immature NK cell number ( J:280182 )

• mice show a 1.3-fold increase in the number of immature CD27+CD11b- NK cells in bone marrow but no significant change in spleen

decreased NK cell number ( J:280182 )

• number of NK cells (Lin-CD122+DX5+) is decreased 4-fold in spleen and 1.5-fold in the bone marrow

decreased mature NK cell number ( J:280182 )

• mice show a 9-fold reduction of mature CD27-CD11b+ NK cells in spleen, and 4-fold loss of CD27-CD11b+ NK cells in the bone marrow

abnormal NK cell physiology ( J:280182 )

• mice show a 2-fold reduction in the % of proliferating Ki67+CD11b- NK cells in the bone marrow, with no change in the % of Ki67+CD11b+ cells

• splenic CD11b- NK cells show no change in Ki67 staining, and even a small increase in the CD11b+ fraction relative to controls (10.8% vs 6.8%)

• annexin V staining showed a modest reduction of cell death CD11b- NK cells in the bone marrow relative to controls (10% vs 14%) but no significant change in splenic CD11b- NK cells

• CD11b+ NK cells show a 2-fold increase in annexin V staining in the bone marrow and a 3-fold increase in spleen

• however, activation of mTOR in response to IL-15 stimulation is normal in immature NK cells

impaired natural killer cell mediated cytotoxicity ( J:280182 )

• mice exhibit reduced clearance of MHC class I-deficient tumors in vivo; 48 h after i.p. injection, recovery of RMA/s tumor cells is 6.5% versus 1.8% in control mice

• in an in vitro YAC-1 target lysis assay, splenic NK cells (DX5+) show 53% of the cytotoxic activity seen in control mice at a 2:1 E:T ratio

hematopoietic system

abnormal NK cell differentiation ( J:280182 )

• mice show subtle changes in the expression of NK cell surface receptors and a defect in NK cell maturation with a 5-fold reduction of CD27+CD11b+ NK cells in spleen and a 2.7-fold loss of CD27+CD11b+ NK cells in the bone marrow

increased immature NK cell number ( J:280182 )

• mice show a 1.3-fold increase in the number of immature CD27+CD11b- NK cells in bone marrow but no significant change in spleen

decreased NK cell number ( J:280182 )

• number of NK cells (Lin-CD122+DX5+) is decreased 4-fold in spleen and 1.5-fold in the bone marrow

decreased mature NK cell number ( J:280182 )

• mice show a 9-fold reduction of mature CD27-CD11b+ NK cells in spleen, and 4-fold loss of CD27-CD11b+ NK cells in the bone marrow

abnormal NK cell physiology ( J:280182 )

• mice show a 2-fold reduction in the % of proliferating Ki67+CD11b- NK cells in the bone marrow, with no change in the % of Ki67+CD11b+ cells

• splenic CD11b- NK cells show no change in Ki67 staining, and even a small increase in the CD11b+ fraction relative to controls (10.8% vs 6.8%)

• annexin V staining showed a modest reduction of cell death CD11b- NK cells in the bone marrow relative to controls (10% vs 14%) but no significant change in splenic CD11b- NK cells

• CD11b+ NK cells show a 2-fold increase in annexin V staining in the bone marrow and a 3-fold increase in spleen

• however, activation of mTOR in response to IL-15 stimulation is normal in immature NK cells

impaired natural killer cell mediated cytotoxicity ( J:280182 )

• mice exhibit reduced clearance of MHC class I-deficient tumors in vivo; 48 h after i.p. injection, recovery of RMA/s tumor cells is 6.5% versus 1.8% in control mice

• in an in vitro YAC-1 target lysis assay, splenic NK cells (DX5+) show 53% of the cytotoxic activity seen in control mice at a 2:1 E:T ratio

Genotype
MGI:6515792

cn4

• Allelic Composition: Sppl3^tm1Itl/Sppl3^em1Pomz; Ncr1^{tm1.1(icre)Viv}/Ncr1⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6 * C57BL/6J

mortality/aging

mortality/aging ( J:280182 )

N • mice are born in Mendelian ratios and survive without any overt phenotype

immune system

abnormal NK cell differentiation ( J:280182 )

• mice show a defect in NK cell maturation with a 1.8-fold reduction of CD27+CD11b+ NK cells in the bone marrow and a 3-fold loss of CD27+CD11b+ NK cells in spleen

increased immature NK cell number ( J:280182 )

• mice show a 1.4-fold increase in the number of immature CD27+CD11b- NK cells in bone marrow but no significant change in spleen

decreased NK cell number ( J:280182 )

• mice exhibit reduced numbers of peripheral NK cells with a 2.9-fold reduction of NK cell number in spleen

• however, NK cell number is normal in the bone marrow

decreased mature NK cell number ( J:280182 )

• mice show a 6-fold reduction of mature CD27-CD11b+ NK cells in spleen, and 3-fold loss of CD27-CD11b+ NK cells in the bone marrow

hematopoietic system

abnormal NK cell differentiation ( J:280182 )

• mice show a defect in NK cell maturation with a 1.8-fold reduction of CD27+CD11b+ NK cells in the bone marrow and a 3-fold loss of CD27+CD11b+ NK cells in spleen

increased immature NK cell number ( J:280182 )

• mice show a 1.4-fold increase in the number of immature CD27+CD11b- NK cells in bone marrow but no significant change in spleen

decreased NK cell number ( J:280182 )

• mice exhibit reduced numbers of peripheral NK cells with a 2.9-fold reduction of NK cell number in spleen

• however, NK cell number is normal in the bone marrow

decreased mature NK cell number ( J:280182 )

• mice show a 6-fold reduction of mature CD27-CD11b+ NK cells in spleen, and 3-fold loss of CD27-CD11b+ NK cells in the bone marrow

Genotype
MGI:6513975

cn5

• Allelic Composition: Rfx7^tm1.1Ggaur/Rfx7^tm1.1Ggaur; Ncr1^{tm1.1(icre)Viv}/Ncr1⁺
• Genetic Background: involves: C57BL/6

immune system

decreased NK cell number ( J:282535 )

• in the spleen, blood, and liver

• mice infected with murine cytomegalovirus exhibit reduced total and Ly49H+ after 1.5 days infection and milder after 3.5 days compared with control mice

abnormal NK cell physiology ( J:282535 )

• decreased survival in vivo and in culture

• treatment with IL2-anti-IL2 complexes partially rescues the ability to reject B2m-deficient splenocytes

• slightly less granzyme A production in uninfected mice

• however, cells exhibit normal cell-mediated immunity when cultured with RMA-S and RMA-H60 target cells

• however, increased IL2 or IL15 amounts restores NK cell survival in culture

increased susceptibility to viral infection ( J:282535 )

• mice infected with murine cytomegalovirus exhibit reduced total and Ly49H+ after 1.5 days infection and milder after 3.5 days with reduced granzyme B production and higher viral loads early after infection in the spleen and lungs compared with control mice

• however, IFNG production from NK cells of cytomegalovirus virus-infected mice

hematopoietic system

decreased NK cell number ( J:282535 )

• in the spleen, blood, and liver

• mice infected with murine cytomegalovirus exhibit reduced total and Ly49H+ after 1.5 days infection and milder after 3.5 days compared with control mice

abnormal NK cell physiology ( J:282535 )

• decreased survival in vivo and in culture

• treatment with IL2-anti-IL2 complexes partially rescues the ability to reject B2m-deficient splenocytes

• slightly less granzyme A production in uninfected mice

• however, cells exhibit normal cell-mediated immunity when cultured with RMA-S and RMA-H60 target cells

• however, increased IL2 or IL15 amounts restores NK cell survival in culture