Phenotypes associated with this allele

• Allele Symbol: Irag1^tm2.1Jens
• Allele Name: targeted mutation 2.1, Jens Schlossmann
• Allele ID: MGI:5292572
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Irag1^tm2.1Jens/Irag1^tm2.1Jens	involves: 129S1/Sv * 129X1/SvJ	MGI:5292656
cx2	Irag1^tm2.1Jens/Irag1^tm2.1Jens Tagln^tm2(PRKG1*)Hfm/Tagln^tm2(PRKG1*)Hfm	involves: 129S1/Sv * 129X1/SvJ	MGI:5292657

Genotype
MGI:5292656

hm1

• Allelic Composition: Irag1^tm2.1Jens/Irag1^tm2.1Jens
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:175887 )

• slightly reduced life expectancy

muscle

abnormal vascular smooth muscle physiology ( J:175887 )

• vascular smooth muscle fail to exhibit NO- and ANP-dependent suppression of intracellular calcium release unlike similarly treated wild-type muscle

impaired muscle relaxation ( J:175887 )

• colon smooth muscle fails to exhibit NO- and cGMP-mediated relaxation unlike in wild-type mice

• phenylephrine-treated aortas fail to exhibit relaxation in response to cGMP unlike in similarly treated wild-type aortas

• NO-treated aortas exhibit a slight reduction in relaxation compared to in similarly treated wild-type aortas

• acetylcholine-induced relaxation of phenylephrine-treated aortas is almost completely suppressed compared to in similarly treated wild-type aortas

• atrial natriuretic peptide (ANP)-induced relaxation of aortas was strongly impaired compared with similarly treated wild-type aortas

digestive/alimentary system

abnormal digestive system morphology ( J:175887 )

• enlarged gastrointestinal tract

cardiovascular system

abnormal systemic arterial blood pressure ( J:175887 )

• LPS-treated mice fail to exhibit a decrease in mean arterial blood pressure unlike in similarly treated wild-type mice

• however, basal mean arterial blood pressure is normal

abnormal vascular smooth muscle physiology ( J:175887 )

• vascular smooth muscle fail to exhibit NO- and ANP-dependent suppression of intracellular calcium release unlike similarly treated wild-type muscle

Genotype
MGI:5292657

cx2

• Allelic Composition: Irag1^tm2.1Jens/Irag1^tm2.1Jens; Tagln^{tm2(PRKG1*)Hfm}/Tagln^{tm2(PRKG1*)Hfm}
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

muscle

impaired muscle relaxation ( J:175887 )

• colon and vascular smooth muscle exhibit reduced cGMP-mediated relaxation compared with similarly treated wild-type smooth muscle