Phenotypes associated with this allele

• Allele Symbol: Mir140^tm1.1Tkob
• Allele Name: targeted mutation 1.1, Tatsuya Kobayashi
• Allele ID: MGI:5086252
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mir140^tm1.1Tkob/Mir140^tm1.1Tkob	involves: 129S4/SvJae * C57BL/6	MGI:6423704
hm2	Mir140^tm1.1Tkob/Mir140^tm1.1Tkob	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:5086264
cn3	Mir140^tm1.1Tkob/Mir140^tm1.1Tkob Pdgfra^tm8Sor/Pdgfra^tm8Sor Tg(Col2a1-cre)1Rsjo/0	involves: 129S4/SvJae * C57BL/6 * FVB/N * SJL	MGI:5086265

Genotype
MGI:6423704

hm1

• Allelic Composition: Mir140^tm1.1Tkob/Mir140^tm1.1Tkob
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

craniofacial

short snout ( J:286036 )

• shorter nose

growth/size/body

short snout ( J:286036 )

• shorter nose

decreased body size ( J:286036 )

Genotype
MGI:5086264

hm2

• Allelic Composition: Mir140^tm1.1Tkob/Mir140^tm1.1Tkob
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

skeleton

abnormal basicranium morphology ( J:174096 )

• at E17.5, mice exhibit a shortening of bones in the basal skull compared with wild-type mice

small basisphenoid bone ( J:174096 )

• at P5.5, mice exhibit a reduction in the longitudinal length of the basisphenoid bone and a shortening of the bidirectional growth plates flanking the bone compared with wild-type mice

decreased cranium height ( J:174096 )

decreased length of long bones ( J:174096 )

short femur ( J:174096 )

short tibia ( J:174096 )

abnormal skeleton development ( J:174096 )

• at E14.5, mineralization of ulna is more advanced than in wild-type mice

• at P1.5, mineralization of the hyoid horn is more advanced than in wild-type mice

• at birth, mice exhibit advanced talus bone development with a large mineralization area than in wild-type mice

• mice exhibit mildly accelerated chondrocyte differentiation and bone development compared to in wild-type mice

• however, columnar chondrocyte proliferation is normal

abnormal long bone epiphyseal plate morphology ( J:174096 )

• the growth plates exhibit increased resting zone and modestly decreased columnar zone compared to in wild-type mice

increased width of hypertrophic chondrocyte zone ( J:174096 )

• at E14.5 in the tibia

• at P0.5 in the talus

• however, columnar chondrocyte proliferation is normal

decreased long bone epiphyseal plate size ( J:174096 )

growth/size/body

decreased body size ( J:174096 )

decreased body weight ( J:174096 )

postnatal growth retardation ( J:174096 )

craniofacial

abnormal basicranium morphology ( J:174096 )

• at E17.5, mice exhibit a shortening of bones in the basal skull compared with wild-type mice

small basisphenoid bone ( J:174096 )

• at P5.5, mice exhibit a reduction in the longitudinal length of the basisphenoid bone and a shortening of the bidirectional growth plates flanking the bone compared with wild-type mice

decreased cranium height ( J:174096 )

limbs/digits/tail

short femur ( J:174096 )

short tibia ( J:174096 )

Genotype
MGI:5086265

cn3

• Allelic Composition: Mir140^tm1.1Tkob/Mir140^tm1.1Tkob; Pdgfra^tm8Sor/Pdgfra^tm8Sor; Tg(Col2a1-cre)1Rsjo/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/N * SJL

skeleton

abnormal skeleton morphology ( J:174096 )

• the skeletal defects observed in Mir140^tm1.1Tkob homozygotes are not rescued

decreased length of long bones ( J:174096 )

• compared with wild-type mice and Mir140^tm1.1Tkob homozygotes

growth/size/body

decreased body size ( J:174096 )