Analysis Tools
homeostasis/metabolism
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• at 8 weeks and 7 months of age, males show a mild increase in fasting serum insulin levels relative to wild-type controls
• however, fasting serum glucose levels and glucose tolerance are normal at both ages
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• at 8 weeks of age, males show decreased levels of circulating HMW adiponectin (high molecular weight oligomer) relative to wild-type controls
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• mass spectrometry of epididymal fat tissue revealed higher cellular levels of adiponectin and other adipokines (increased retention implying reduced secretion) relative to wild-type controls
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• at 7 months of age, males show a significant reduction of collagen deposition in epididymal adipose tissue, as determined by collagen Masson's trichrome staining
• mass spectrometry of epididymal adipose revealed lower levels of 14 collagen isoforms including collagen chains 6a1, a2, a3 & a5, the most abundant species found in adipose tissue
• total collagen content of adipose tissue is reduced by ~40%, as quantified by a Sirius Red Total Collagen Detection Kit
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adipose tissue
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• at 7 months of age, epididymal adipose tissue is virtually free from fibrosis due to decreased collagen deposition, unlike in wild-type controls
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• at 8 weeks of age, males show increased expression of inflammatory markers (F4/80, TNF, and CD11b) in adipose tissue relative to wild-type controls
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cellular
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• at 8 weeks of age, males show increased expression of several ER stress markers in whole adipose tissue lysates, indicating mild adipocyte ER stress
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immune system
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• at 8 weeks of age, males show increased expression of inflammatory markers (F4/80, TNF, and CD11b) in adipose tissue relative to wild-type controls
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growth/size/body
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• at 8 weeks and 7 months of age, males show normal body weight and body composition (lean and fat body mass) relative to wild-type controls
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