Phenotypes associated with this allele

• Allele Symbol: Lrig1^{tm1.1(cre/ERT2)Rjc}
• Allele Name: targeted mutation 1.1, Robert J Coffey
• Allele ID: MGI:4947937
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^tm1.1(cre/ERT2)Rjc	involves: 129 * C57BL/6	MGI:5432134
cn2	Scd1^tm2Ntam/Scd1^tm2Ntam Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	B6.129(Cg)-Lrig1^tm1.1(cre/ERT2)Rjc Scd1^tm2Ntam	MGI:7287840
cn3	Apc^tm2.1Cip/Apc^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	involves: 129 * 129P2/OlaHsd * C57BL/6	MGI:5432136
cn4	Braf^tm1Mmcm/Braf^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6J	MGI:8332036
cn5	Gt(ROSA)26Sor^tm3(CAG-KIT*)Geno/Gt(ROSA)26Sor^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	involves: 129S6/SvEvTac * C57BL/6	MGI:8332048
cx6	Lgr5^tm1(cre/ERT2)Cle/Lgr5^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^tm1.1(cre/ERT2)Rjc	involves: 129 * 129P2/OlaHsd * C57BL/6	MGI:5432135

Genotype
MGI:5432134

hm1

• Allelic Composition: Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1^{tm1.1(cre/ERT2)Rjc}
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:186084 )

• homozygotes (and heterozygotes) are viable and fertile

Genotype
MGI:7287840

cn2

• Allelic Composition: Scd1^tm2Ntam/Scd1^tm2Ntam; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1⁺
• Genetic Background: B6.129(Cg)-Lrig1^{tm1.1(cre/ERT2)Rjc} Scd1^tm2Ntam

integument

abnormal hair shaft morphology ( J:325761 )

• decreased hair shaft

abnormal hair follicle matrix region morphology ( J:325761 )

• loss of matrix progenitors

decreased hair follicle number ( J:325761 )

hair follicle degeneration ( J:325761 )

abnormal dermis papillary layer morphology ( J:325761 )

• loss of dermal papilla cells

Genotype
MGI:5432136

cn3

• Allelic Composition: Apc^tm2.1Cip/Apc⁺; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1⁺
• Genetic Background: involves: 129 * 129P2/OlaHsd * C57BL/6

neoplasm

increased intestinal adenoma incidence ( J:186084 )

• with tamoxifen treatement, colon tumors are observed by day 50 following Apc^fldeletion with cre induction (and stochastic loss of second Apc allele)

• by day 50 there are multiple distal colonic tumors with no proximal colon tumor burden; tumor phenotype is 100% penetrant

• largest tumors are found in distal colon, many having high grade dysplasia; highest tumor number is observed in jejunum

digestive/alimentary system

increased intestinal adenoma incidence ( J:186084 )

• with tamoxifen treatement, colon tumors are observed by day 50 following Apc^fldeletion with cre induction (and stochastic loss of second Apc allele)

• by day 50 there are multiple distal colonic tumors with no proximal colon tumor burden; tumor phenotype is 100% penetrant

• largest tumors are found in distal colon, many having high grade dysplasia; highest tumor number is observed in jejunum

Genotype
MGI:8332036

cn4

• Allelic Composition: Braf^tm1Mmcm/Braf⁺; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6J

neoplasm

increased oral papilloma incidence ( J:300991 )

• mice develop oral tumors 12 weeks after a single injection of tamoxifen at 8 weeks of age; tumors are squamous papillomas

increased gastrointestinal stromal tumor incidence ( J:300991 )

• the outer longitudinal muscular layer is almost completely replaced by lesions with diffuse CD34 staining, indicating gastrointestinal stromal tumors (GIST) and active proliferation of the hyperplastic lesions is seen

• similar lesions are seen in the small intestine and colon mostly in the outer longitudinal muscular layer, although less prominently than in the stomach

behavior/neurological

abnormal food intake ( J:300991 )

• mice treated with tamoxifen are unable to eat due to development of oral tumors

cellular

interstitial cells of Cajal hyperplasia ( J:300991 )

• hyperplastic regions are devoid of smooth muscle actin and partially positive for KIT, resembling interstitial cells of Cajal (ICC) hyperplasia in the gastric wall

craniofacial

increased oral papilloma incidence ( J:300991 )

• mice develop oral tumors 12 weeks after a single injection of tamoxifen at 8 weeks of age; tumors are squamous papillomas

digestive/alimentary system

interstitial cells of Cajal hyperplasia ( J:300991 )

• hyperplastic regions are devoid of smooth muscle actin and partially positive for KIT, resembling interstitial cells of Cajal (ICC) hyperplasia in the gastric wall

abnormal stomach wall morphology ( J:300991 )

• 8-10 weeks after tamoxifen administration, mice show a thickened gastric wall

abnormal stomach non-glandular epithelium morphology ( J:300991 )

• squamous hyperplasia of the forestomach in tamoxifen-treated mice

abnormal stomach muscularis externa morphology ( J:300991 )

• the outer half of the muscularis propria is replaced by hyperplastic spindle-shaped cells with dysplastic features in tamoxifen-treated mice

increased gastrointestinal stromal tumor incidence ( J:300991 )

• the outer longitudinal muscular layer is almost completely replaced by lesions with diffuse CD34 staining, indicating gastrointestinal stromal tumors (GIST) and active proliferation of the hyperplastic lesions is seen

• similar lesions are seen in the small intestine and colon mostly in the outer longitudinal muscular layer, although less prominently than in the stomach

growth/size/body

increased oral papilloma incidence ( J:300991 )

• mice develop oral tumors 12 weeks after a single injection of tamoxifen at 8 weeks of age; tumors are squamous papillomas

muscle

abnormal stomach muscularis externa morphology ( J:300991 )

• the outer half of the muscularis propria is replaced by hyperplastic spindle-shaped cells with dysplastic features in tamoxifen-treated mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
gastrointestinal stromal tumor		DOID:9253	OMIM:606764	J:300991

Genotype
MGI:8332048

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm3(CAG-KIT*)Geno}/Gt(ROSA)26Sor⁺; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

digestive/alimentary system

increased gastrointestinal stromal tumor incidence ( J:300991 )

• adult mice given a single dose of tamoxifen exhibit small gastrointestinal stromal tumors (GIST)-like lesions in the muscularis propria of the stomach and intestine 1 month after treatment

neoplasm

increased gastrointestinal stromal tumor incidence ( J:300991 )

• adult mice given a single dose of tamoxifen exhibit small gastrointestinal stromal tumors (GIST)-like lesions in the muscularis propria of the stomach and intestine 1 month after treatment

Genotype
MGI:5432135

cx6

• Allelic Composition: Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1^{tm1.1(cre/ERT2)Rjc}
• Genetic Background: involves: 129 * 129P2/OlaHsd * C57BL/6

neoplasm

increased intestinal adenoma incidence ( J:186084 )

• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)

• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:186084 )

• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1

increased intestinal adenoma incidence ( J:186084 )

• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)

• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:186084 )

• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1