About   Help   FAQ
Targeted Allele Detail
Symbol: Braftm1Mmcm
Name: Braf transforming gene; targeted mutation 1, Martin McMahon
MGI ID: MGI:3711771
Synonyms: BrafCA, BrafCA-V600E, BRAFF-V600E, BRAFV600Eca
Gene: Braf  Location: Chr6:39580171-39702397 bp, - strand  Genetic Position: Chr6, 18.43 cM
Alliance: Braftm1Mmcm page
Germline Transmission:  Earliest citation of germline transmission: J:121715
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert a loxP site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a floxed neo cassette. A modified exon 15 was engineered into the vector to encode BrafV600E and a silent XbaI restriction site. RT-PCR confirmed that mutant transcript was expressed at the same level as wild-type, and that the V600E mutation was not detected. (J:121715)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 11 anatomical structures
Tumor Data
List all tumor models in MMHCdb carrying Braftm1Mmcm
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Braf Mutation:  45 strains or lines available
Original:  J:121715 Dankort D, et al., A new mouse model to explore the initiation, progression, and therapy of BRAFV600E-induced lung tumors. Genes Dev. 2007 Feb 15;21(4):379-84
All:  180 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
MGI 6.22
The Jackson Laboratory