Phenotypes associated with this allele

• Allele Symbol: Rapgef2^tm1.2Hous
• Allele Name: targeted mutation 1.2, Steven X Hou
• Allele ID: MGI:4839129
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rapgef2^tm1.2Hous/Rapgef2^tm1.2Hous	involves: 129S1/Sv	MGI:4839183

Genotype
MGI:4839183

hm1

• Allelic Composition: Rapgef2^tm1.2Hous/Rapgef2^tm1.2Hous
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:165879 )

• mice die between E11.5 and E12.5

hematopoietic system

abnormal embryonic hematopoiesis ( J:165879 )

• at E10.5, altered marker expression indicates impaired maintenance of definitive hematopoiesis unlike in wild-type mice

• differentiation of hematopoietic progenitors in vitro is inhibited compared with wild-type cells

• however, placenta hematopoiesis in normal

decreased common myeloid progenitor cell number ( J:165879 )

• E10.5 embryos and yolk sac form 4- to 5-fold fewer mixed colony-forming units and granulocyte/macrophage colony-forming units compared with wild-type mice

abnormal erythropoiesis ( J:165879 )

• at E10.5, the number of nucleated erythroid cells is reduced compared to in wild-type mice

decreased erythroid progenitor cell number ( J:165879 )

absent erythrocytes ( J:165879 )

• at E10.5, mice lack enucleated erythroid cells unlike wild-type mice in which very few enucleated erythroid cells are detected

decreased hematopoietic stem cell number ( J:165879 )

• in embryos and the yolk sac

abnormal hematopoietic stem cell physiology ( J:165879 )

• hematopoietic progenitor cells exhibit impaired self-renewal capacity compared with wild-type cells

• hematopoietic progenitor cells exhibit impaired in vitro expansion and differentiation into hematopoietic lineages compared with wild-type cells

cardiovascular system

abnormal vascular development ( J:165879 )

absent vitelline blood vessels ( J:165879 )

abnormal heart development ( J:165879 )

cellular

abnormal cell adhesion ( J:165879 )

abnormal cell migration ( J:165879 )

• cells from the yolk sac and embryos exhibit reduced migration in vitro compared with wild-type cells

decreased cell proliferation ( J:165879 )

• of cells from the yolk sac and embryos

growth/size/body

decreased embryo size ( J:165879 )

• at E9.5 to E10.5

embryo

absent vitelline blood vessels ( J:165879 )

decreased embryo size ( J:165879 )

• at E9.5 to E10.5

abnormal embryonic hematopoiesis ( J:165879 )

• at E10.5, altered marker expression indicates impaired maintenance of definitive hematopoiesis unlike in wild-type mice

• differentiation of hematopoietic progenitors in vitro is inhibited compared with wild-type cells

• however, placenta hematopoiesis in normal

digestive/alimentary system

abnormal digestive system development ( J:165879 )

liver/biliary system

abnormal liver development ( J:165879 )

nervous system

abnormal brain development ( J:165879 )

integument

translucent skin ( J:165879 )

• at E9.5 to E10.5, mice are translucent and appear anemic unlike wild-type mice