Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2.1Flv mutation
(0 available);
any
Rad50 mutation
(53 available)
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immune system
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• from CD4+ T cells cultured under in vitro Th2-polarizing conditions with anti-CD3 antibodies
• from splenocytes of mice stimulated with ovalbumin and restimulated in vitro with ovalbumin
• from skin draining lymph nodes of mice subjected to epicutaneous priming followed by restimulation with ovalbumin in the presence of splenic antibody presenting cells
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• from CD4+ T cells cultured under in vitro Th2-polarizing conditions with anti-CD3 antibodies
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• from CD4+ T cells cultured under in vitro Th2-polarizing conditions with anti-CD3 antibodies
• from splenocytes of mice stimulated with ovalbumin and restimulated in vitro with ovalbumin
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2.1Flv mutation
(0 available);
any
Rad50 mutation
(53 available)
Rad50tm2.2Flv mutation
(0 available);
any
Rad50 mutation
(53 available)
Tg(Cd4-cre)1Cwi mutation
(10 available)
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immune system
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• CD4 T cell differentiation is normal
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• stimulated CD4+ T cells are larger than similarly treated wild-type cells
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• in ovalbumin challenged mice
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• under Th1 conditions, splenocytes produce less IFN-gamma than similarly treated wild-type cells
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• under Th2 conditions, splenocytes produce less IL4, IL5, and IL13 compared with similarly treated wild-type cells
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• in splenocytes under Th1 conditions
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• in splenocytes under Th2 conditions
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• in splenocytes under Th2 conditions
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• in splenocytes under Th2 conditions
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• ovalbumin-treated mice exhibit no increase in eosinophils and lymphocytes in the bronchoalveolar lavage fluid, reduced infiltration of cells in the peribronchiolar and perivascular regions of the lung and decreased mucus production unlike wild-type mice
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respiratory system
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• following methacholine treatment, ovalbumin sensitized and challenged mice fail to exhibit an increase in airway resistance unlike similarly treated wild-type mice
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• following methacholine treatment, ovalbumin sensitized and challenged mice fail to exhibit airway hyperresponsiveness unlike similarly treated wild-type mice
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cellular
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• Th2 cells exhibit a dramatic increase in DNA methylation in the il4 promoter and CNS-1 region unlike similarly treated wild-type cells that exhibit a decrease in DNA methylation in this region
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hematopoietic system
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• stimulated CD4+ T cells are larger than similarly treated wild-type cells
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• in ovalbumin challenged mice
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• under Th1 conditions, splenocytes produce less IFN-gamma than similarly treated wild-type cells
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• under Th2 conditions, splenocytes produce less IL4, IL5, and IL13 compared with similarly treated wild-type cells
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Analysis Tools