Phenotypes associated with this allele

• Allele Symbol: Mei4^tm1Bdm
• Allele Name: targeted mutation 1, Bernard de Massy
• Allele ID: MGI:4818799
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mei4^tm1Bdm/Mei4^tm1Bdm	involves: 129S2/SvPas	MGI:4818913

Genotype
MGI:4818913

hm1

• Allelic Composition: Mei4^tm1Bdm/Mei4^tm1Bdm
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

azoospermia ( J:161203 )

♂ • no spermatozoa in the epididymis of 8 week old males

♂ • no post meiotic cells in the seminiferous tubules

abnormal spermatocyte morphology ( J:276412 )

♂ • both focus and aggregate formation of ANKRD31, REC114, and MEI4 are disrupted in spermatocytes

abnormal meiosis ( J:161203 )

abnormal chromosomal synapsis ( J:161203 )

arrest of male meiosis ( J:161203 )

♂ • spermatocyte chromosomes normal at leptolema stage but abnormal at zygotene stage

♂ • no nuclei with fully synapsed chromosomes

♂ • defect in forming double strand breaks

♂ • progress of meiosis ends by mid pachytene stage

abnormal synaptonemal complex ( J:161203 )

increased testis apoptosis ( J:161203 )

♂ • increased apoptosis in the testes

embryo

embryo phenotype ( J:161203 )

N • homozygote development is normal

reproductive system

increased testis apoptosis ( J:161203 )

♂ • increased apoptosis in the testes

abnormal reproductive system morphology ( J:161203 )

abnormal ovarian follicle number ( J:161203 )

♀ • few follicles in ovaries at 8 weeks of age

decreased primary ovarian follicle number ( J:161203 )

♀ • ovaries are nearly devoid of primary follicles at 2 weeks of age

decreased primordial ovarian follicle number ( J:161203 )

♀ • ovaries are nearly devoid of primordial follicles at 2 weeks of age

abnormal gametogenesis ( J:161203 )

azoospermia ( J:161203 )

♂ • no spermatozoa in the epididymis of 8 week old males

♂ • no post meiotic cells in the seminiferous tubules

abnormal spermatocyte morphology ( J:276412 )

♂ • both focus and aggregate formation of ANKRD31, REC114, and MEI4 are disrupted in spermatocytes

abnormal meiosis ( J:161203 )

abnormal chromosomal synapsis ( J:161203 )

arrest of male meiosis ( J:161203 )

♂ • spermatocyte chromosomes normal at leptolema stage but abnormal at zygotene stage

♂ • no nuclei with fully synapsed chromosomes

♂ • defect in forming double strand breaks

♂ • progress of meiosis ends by mid pachytene stage

abnormal synaptonemal complex ( J:161203 )

abnormal internal male genitalia morphology ( J:161203 )

♂

abnormal testis morphology ( J:161203 )

♂

abnormal seminiferous tubule morphology ( J:161203 )

♂ • no post meiotic cells in the seminiferous tubules

♂ • some primary spermatocytes found

decreased testis weight ( J:161203 )

♂ • testes weigh 50-70% less than those of control mice

abnormal epididymis morphology ( J:161203 )

♂ • no spermatozoa in the epididymis of 8 week old males

endocrine/exocrine glands

increased testis apoptosis ( J:161203 )

♂ • increased apoptosis in the testes

abnormal ovarian follicle number ( J:161203 )

♀ • few follicles in ovaries at 8 weeks of age

decreased primary ovarian follicle number ( J:161203 )

♀ • ovaries are nearly devoid of primary follicles at 2 weeks of age

decreased primordial ovarian follicle number ( J:161203 )

♀ • ovaries are nearly devoid of primordial follicles at 2 weeks of age

abnormal testis morphology ( J:161203 )

♂

abnormal seminiferous tubule morphology ( J:161203 )

♂ • no post meiotic cells in the seminiferous tubules

♂ • some primary spermatocytes found

decreased testis weight ( J:161203 )

♂ • testes weigh 50-70% less than those of control mice