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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Hand2tm1.2Zllr
targeted mutation 1.2, Rolf Zeller
MGI:4453961
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Hand2tm1.2Zllr/Hand2tm1.2Zllr involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL MGI:4453964
cn2
 		Gli3Xt-J/Gli3Xt-J
Hand2tm1.1Zllr/Hand2tm1.2Zllr
Tg(Prrx1-cre)1Cjt/0 		involves: 129 * BALB/cJ * C3H/HeJ * C57BL/6 * SJL MGI:4453963
cn3
 		Hand2tm1.1Zllr/Hand2tm1.2Zllr
Tg(Prrx1-cre)1Cjt/0 		involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL MGI:4453962
cx4
 		Hand2tm1.2Zllr/Hand2+
Is(In8B2-8B3.1;6C1)1Tshir/0 		involves: 129 * C57BL/6 * C57BL/10 * NZB * wild-derived MGI:5518834
cx5
 		Hand2tm1.2Zllr/Hand2tm1.2Zllr
Is(In8B2-8B3.1;6C1)1Tshir/Is(In8B2-8B3.1;6C1)1Tshir 		involves: 129 * C57BL/6 * C57BL/10 * NZB * wild-derived MGI:5518837


Genotype
MGI:4453964
hm1
Allelic
Composition		 Hand2tm1.2Zllr/Hand2tm1.2Zllr
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hand2tm1.2Zllr mutation (0 available); any Hand2 mutation (14 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
growth/size/body
embryonic growth retardation ( J:159210 )
• growth retardation at E9.5-9.75 (25-27 somites)

cardiovascular system
abnormal pharyngeal arch artery morphology ( J:159210 )
• malformed branchial arches at embryonic day E9.5-9.75
dilated aortic sac ( J:159210 )
• dilated aortic sac at E9.5-9.75
distended pericardium ( J:159210 )
• dilated pericardial sac at E9.5-9.75

cellular
increased embryonic tissue cell apoptosis ( J:159210 )
• massive and generalized cell death in embryos and limb buds at E9.5

craniofacial
abnormal pharyngeal arch artery morphology ( J:159210 )
• malformed branchial arches at embryonic day E9.5-9.75

embryo
abnormal pharyngeal arch artery morphology ( J:159210 )
• malformed branchial arches at embryonic day E9.5-9.75
increased embryonic tissue cell apoptosis ( J:159210 )
• massive and generalized cell death in embryos and limb buds at E9.5
embryonic growth retardation ( J:159210 )
• growth retardation at E9.5-9.75 (25-27 somites)




Genotype
MGI:4453963
cn2
Allelic
Composition		 Gli3Xt-J/Gli3Xt-J
Hand2tm1.1Zllr/Hand2tm1.2Zllr
Tg(Prrx1-cre)1Cjt/0
Genetic
Background		 involves: 129 * BALB/cJ * C3H/HeJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gli3Xt-J mutation (3 available); any Gli3 mutation (84 available)
Hand2tm1.1Zllr mutation (0 available); any Hand2 mutation (14 available)
Hand2tm1.2Zllr mutation (0 available); any Hand2 mutation (14 available)
Tg(Prrx1-cre)1Cjt mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
limbs/digits/tail
abnormal autopod morphology ( J:159210 )
• digits with undetermined identities in forelimb autopod at E14.5
postaxial polydactyly ( J:159210 )
• extreme postaxial polydactyly in forelimb autopod at E14.5
preaxial polydactyly ( J:159210 )
• extreme preaxial polydactyly in forelimb autopod at E14.5
abnormal forelimb stylopod morphology ( J:159210 )
• shortening of the stylopod in forelimbs at E14.5
abnormal forelimb zeugopod morphology ( J:159210 )
• symmetrical forelimb zeugopodal bones and elbow joints at E14.5
abnormal limb development ( J:159210 )
• duplicated elbow-like structure in forelimbs at E14.5
• stunted forelimbs at E14.5
• little phenotypic variability
• survival of the zeugopod and autopod progenitors is restored in contrast to Tg(Prrx1-cre)1Cjt/o, Hand2tm1.1Zllr, Hand2tm1.2Zllr limbs




Genotype
MGI:4453962
cn3
Allelic
Composition		 Hand2tm1.1Zllr/Hand2tm1.2Zllr
Tg(Prrx1-cre)1Cjt/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hand2tm1.1Zllr mutation (0 available); any Hand2 mutation (14 available)
Hand2tm1.2Zllr mutation (0 available); any Hand2 mutation (14 available)
Tg(Prrx1-cre)1Cjt mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
limbs/digits/tail
abnormal autopod morphology ( J:159210 )
• distal truncations of the forelimb skeleton and loss of the autopod in most severely affected cases at E14.5 (35%)
• fused tarsal bones in hindlimb at E14.5
abnormal forelimb zeugopod morphology ( J:159210 )
• variable skeletal phenotypes at E14.5
• shortened and fused radius and ulna in most severely affected cases (35%)
• formation of two misplaced zeugopodal bones (39%) in the most hypomorphic phenotype (weak)
• formation of one zeugopodal bone (26%) in the less hypomorphic phenotype (intermediate)
abnormal hindlimb morphology ( J:159210 )
• formation of an autopod with 4-5 digits in hindlimb at E14.5
• the formation of digit 2 and/or 3 in hindlimb is always affected
abnormal digit development ( J:159210 )
• variable skeletal phenotypes in forelimb at E14.5
• distal truncations of the forelimb skeleton and loss of the autopod in most severely affected cases at E14.5 (35%)
• formation of three anterior digits and a hypoplastic digit that resembles digit 4 (39%) in the most hypomorphic phenotype (weak) in forelimb
• formation of two digits (26%) in the less hypomorphic phenotype (intermediate) in forelimb
• formation of an autopod with 4-5 digits in hindlimb at E14.5
• the formation of digit 2 and/or 3 in hindlimb is always affected
abnormal interdigital cell death ( J:159210 )
• in the most severely affected forelimb buds, the apoptotic domain expands distal-anterior along the entire proximo-distal axis at E10.25, E10.75 and E11

cellular
abnormal interdigital cell death ( J:159210 )
• in the most severely affected forelimb buds, the apoptotic domain expands distal-anterior along the entire proximo-distal axis at E10.25, E10.75 and E11
increased apoptosis ( J:159210 )
• in the most severely affected forelimb buds, the apoptotic domain expands distal-anterior along the entire proximo-distal axis at E10.25, E10.75 and E11




Genotype
MGI:5518834
cx4
Allelic
Composition		 Hand2tm1.2Zllr/Hand2+
Is(In8B2-8B3.1;6C1)1Tshir/0
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/10 * NZB * wild-derived
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hand2tm1.2Zllr mutation (0 available); any Hand2 mutation (14 available)
Is(In8B2-8B3.1;6C1)1Tshir mutation (1 available); any Is(In8B2-8B3.1;6C1)1Tshir mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
limbs/digits/tail
limbs/digits/tail phenotype ( J:198239 )
N
• mutants do not exhibit first digit deformities that are seen in Is(In8B2-8B3.1;6C1)1Tshir/0 mice




Genotype
MGI:5518837
cx5
Allelic
Composition		 Hand2tm1.2Zllr/Hand2tm1.2Zllr
Is(In8B2-8B3.1;6C1)1Tshir/Is(In8B2-8B3.1;6C1)1Tshir
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/10 * NZB * wild-derived
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hand2tm1.2Zllr mutation (0 available); any Hand2 mutation (14 available)
Is(In8B2-8B3.1;6C1)1Tshir mutation (1 available); any Is(In8B2-8B3.1;6C1)1Tshir mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:198239 )
N
• mutants do not exhibit ventricular septal defect

craniofacial
abnormal craniofacial morphology ( J:198239 )
• craniofacial deformities

limbs/digits/tail
limbs/digits/tail phenotype ( J:198239 )
N
• mutants do not exhibit hemimelia or digit deformities

skeleton
abnormal cervical vertebrae morphology ( J:198239 )
• cervical vertebra anomalies

growth/size/body
microcephaly ( J:198239 )




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Send questions and comments to User Support. 		last database update
09/30/2025
MGI 6.24 		The Jackson Laboratory