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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tra2btm1.1Bwir
targeted mutation 1.1, Brunhilde Wirth
MGI:4450919
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Tra2btm1.1Bwir/Tra2btm1.1Bwir involves: 129S4/SvJae * C57BL/6 * C57BL/6N * SJL MGI:4450952
cn2
 		Tra2btm1.1Bwir/Tra2btm1.1Bwir
Tg(Ddx4-cre)1Dcas/0 		involves: 129S4/SvJae * C57BL/6J * C57BL/6N * SJL MGI:8190662
cn3
 		Tra2btm1.1Bwir/Tra2b+
Tg(Ddx4-cre)1Dcas/0 		involves: 129S4/SvJae * C57BL/6J * C57BL/6N * SJL MGI:8190664


Genotype
MGI:4450952
hm1
Allelic
Composition		 Tra2btm1.1Bwir/Tra2btm1.1Bwir
Genetic
Background		 involves: 129S4/SvJae * C57BL/6 * C57BL/6N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tra2btm1.1Bwir mutation (0 available); any Tra2b mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:159448 )
• mice are fertile with a normal life span




Genotype
MGI:8190662
cn2
Allelic
Composition		 Tra2btm1.1Bwir/Tra2btm1.1Bwir
Tg(Ddx4-cre)1Dcas/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6J * C57BL/6N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ddx4-cre)1Dcas mutation (2 available)
Tra2btm1.1Bwir mutation (0 available); any Tra2b mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
azoospermia ( J:360846 )
• adult males (10-18 weeks of age) show complete absence of epididymal sperm
abnormal spermatogonia morphology ( J:360846 )
• at P1, nuclear TRA2B protein is absent from spermatogonia but readily detectable within adjacent Sertoli cells
decreased spermatogonia number ( J:360846 )
• in adult males, the small testis phenotype is attributed to reduced proliferation and demise of the spermatogonia
abnormal spermatogonia proliferation ( J:360846 )
• although males are born with normal numbers of male germ cells, the number of testicular RBMY-positive germ cells is progressively decreased between P0 and P3
abnormal seminiferous tubule morphology ( J:360846 )
• hematoxylin-stained adult testes exhibit a complete absence of male germ cells, consistent with a "Sertoli-cell only" phenotype
small testis ( J:360846 )
• adult testes are markedly smaller than wild type testes
decreased testis weight ( J:360846 )
• adult males show severely decreased testis:body weight ratios

cellular
azoospermia ( J:360846 )
• adult males (10-18 weeks of age) show complete absence of epididymal sperm
abnormal spermatogonia morphology ( J:360846 )
• at P1, nuclear TRA2B protein is absent from spermatogonia but readily detectable within adjacent Sertoli cells
decreased spermatogonia number ( J:360846 )
• in adult males, the small testis phenotype is attributed to reduced proliferation and demise of the spermatogonia
abnormal spermatogonia proliferation ( J:360846 )
• although males are born with normal numbers of male germ cells, the number of testicular RBMY-positive germ cells is progressively decreased between P0 and P3

endocrine/exocrine glands
abnormal seminiferous tubule morphology ( J:360846 )
• hematoxylin-stained adult testes exhibit a complete absence of male germ cells, consistent with a "Sertoli-cell only" phenotype
small testis ( J:360846 )
• adult testes are markedly smaller than wild type testes
decreased testis weight ( J:360846 )
• adult males show severely decreased testis:body weight ratios




Genotype
MGI:8190664
cn3
Allelic
Composition		 Tra2btm1.1Bwir/Tra2b+
Tg(Ddx4-cre)1Dcas/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6J * C57BL/6N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ddx4-cre)1Dcas mutation (2 available)
Tra2btm1.1Bwir mutation (0 available); any Tra2b mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
oligozoospermia ( J:360846 )
• adult males (10-18 weeks of age) show a significant reduction in epididymal sperm count
small testis ( J:360846 )
• adult testes are significantly smaller than wild type testes
decreased testis weight ( J:360846 )
• adult males show significantly reduced testis:body weight ratios

cellular
oligozoospermia ( J:360846 )
• adult males (10-18 weeks of age) show a significant reduction in epididymal sperm count

endocrine/exocrine glands
small testis ( J:360846 )
• adult testes are significantly smaller than wild type testes
decreased testis weight ( J:360846 )
• adult males show significantly reduced testis:body weight ratios




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory