Phenotypes associated with this allele

• Allele Symbol: Cgas^{tm1a(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:4435548
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cgas^tm1a(EUCOMM)Hmgu/Cgas^tm1a(EUCOMM)Hmgu	C57BL/6N-Cgas^tm1a(EUCOMM)Hmgu	MGI:5548206
hm2	Cgas^tm1a(EUCOMM)Hmgu/Cgas^tm1a(EUCOMM)Hmgu	C57BL/6N-Cgas^tm1a(EUCOMM)Hmgu/Ics	MGI:5782127
hm3	Cgas^tm1a(EUCOMM)Hmgu/Cgas^tm1a(EUCOMM)Hmgu	involves: C57BL/6N	MGI:5789965
cx4	Cgas^tm1a(EUCOMM)Hmgu/Cgas^tm1a(EUCOMM)Hmgu Trex1^tm1Tld/Trex1^tm1Tld	involves: 129P2/OlaHsd * C57BL/6N	MGI:5789966
cx5	Cgas^tm1a(EUCOMM)Hmgu/Cgas^tm1a(EUCOMM)Hmgu Rnaseh2a^tm1Crh/Rnaseh2a^tm1Crh	involves: C57BL/6N	MGI:6274941

Genotype
MGI:5548206

hm1

• Allelic Composition: Cgas^{tm1a(EUCOMM)Hmgu}/Cgas^{tm1a(EUCOMM)Hmgu}
• Genetic Background: C57BL/6N-Cgas^{tm1a(EUCOMM)Hmgu}
• Cell Lines: HEPD0516_3_C04

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:201167 )

• most mice die a few hours after symptoms (ataxia and paralysis) appear at either 3 (4 of 5) or 4 (1 of 5) days post HSV1 infection; while only 3 of 5 wild-type mice die by 6 days after infection

immune system

decreased circulating interferon-alpha level ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

decreased circulating interferon-beta level ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

abnormal immune cell physiology ( J:201167 )

• lung fibroblasts transfected with interferon stimulatory DNA or herring testis DNA fail to produce any detectable level of IFN-beta

• induction of IFN-beta in lung fibroblasts infected with the DNA viruses; herpes simplex virus 1 (HSV1), vaccinia virus (VACV) and a mutant strain of HSV1called d109 is largely abolished

abnormal macrophage physiology ( J:201167 )

• production of IFN-beta and TNF in bone marrow derived macrophages transfected with interferon stimulatory DNA or herring testis DNA are defective

• induction of IFN-beta in l bone marrow derived macrophages infected with VACV or HSV1 strains d109 or 7134 is largely abolished

• induction of IFN-beta in l bone marrow derived macrophages infected with HSV1 is severely but not completely blocked

abnormal dendritic cell physiology ( J:201167 )

• cultured conventional dendritic cells fail to induce IFN-alpha or IFN-beta following transfection with interferon stimulatory DNA or herring testis DNA

• cultured conventional dendritic cells fail to induce IFN-beta following infections with HSV1 strain d109 or VACV and induction by wild-type HSV1 is partially inhibited

abnormal plasmacytoid dendritic cell physiology ( J:201167 )

• induction of IFN-alpha and IFN-beta by interferon stimulatory DNA, poly[dA:dT], and genomic DNA from E. coli and Vibrio cholerae in the presence of liposome is abolished

increased susceptibility to Herpesvirales infection ( J:201167 )

• following intravenous infection with HSV1, levels of IFN-alpha and IFN-beta are reduced, 4 of 5 mice develop ataxia and paralysis by 3 days after infection

• high levels of HSV1 are found in the brain at day 3 after infection in mutants but not in wild-type controls

increased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:201167 )

• most mice die a few hours after symptoms (ataxia and paralysis) appear at either 3 (4 of 5) or 4 (1 of 5) days post HSV1 infection; while only 3 of 5 wild-type mice die by 6 days after infection

homeostasis/metabolism

decreased circulating interferon-alpha level ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

decreased circulating interferon-beta level ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

hematopoietic system

abnormal macrophage physiology ( J:201167 )

• production of IFN-beta and TNF in bone marrow derived macrophages transfected with interferon stimulatory DNA or herring testis DNA are defective

• induction of IFN-beta in l bone marrow derived macrophages infected with VACV or HSV1 strains d109 or 7134 is largely abolished

• induction of IFN-beta in l bone marrow derived macrophages infected with HSV1 is severely but not completely blocked

Genotype
MGI:5782127

hm2

• Allelic Composition: Cgas^{tm1a(EUCOMM)Hmgu}/Cgas^{tm1a(EUCOMM)Hmgu}
• Genetic Background: C57BL/6N-Cgas^{tm1a(EUCOMM)Hmgu}/Ics
• Cell Lines: HEPD0516_3_C04

Data Sources

IMPC Data for Cgas

behavior/neurological

abnormal behavior ( J:165965 )

IMPC - ICS

decreased grip strength ( J:165965 )

IMPC - ICS

abnormal tail movements ( J:165965 )

♀

IMPC - ICS

Genotype
MGI:5789965

hm3

• Allelic Composition: Cgas^{tm1a(EUCOMM)Hmgu}/Cgas^{tm1a(EUCOMM)Hmgu}
• Genetic Background: involves: C57BL/6N

immune system

abnormal interferon secretion ( J:234263 )

• production of type I interferon is reduced in macrophages following transfection with dsDNA (calf thymus DNA)

• production of type I interferon is reduced in MEFs following transfection with dsDNA (calf thymus DNA)

Genotype
MGI:5789966

cx4

• Allelic Composition: Cgas^{tm1a(EUCOMM)Hmgu}/Cgas^{tm1a(EUCOMM)Hmgu}; Trex1^tm1Tld/Trex1^tm1Tld
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6N

immune system

increased susceptibility to Retroviridae infection ( J:234263 )

• increase in fraction of infected bone marrow derived macrophages following lentivirus infection as compared to wild type or Trex1^tm1Tld only controls

Genotype
MGI:6274941

cx5

• Allelic Composition: Cgas^{tm1a(EUCOMM)Hmgu}/Cgas^{tm1a(EUCOMM)Hmgu}; Rnaseh2a^tm1Crh/Rnaseh2a^tm1Crh
• Genetic Background: involves: C57BL/6N

immune system

immune system phenotype ( J:232933 )

N • expression of interferon-stimulated genes is restored to normal levels