Phenotypes associated with this allele

• Allele Symbol: Grik4^tm1(cre)Ksak
• Allele Name: targeted mutation 1, Kenji Sakimura
• Allele ID: MGI:4360478
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Grik4^tm1(cre)Ksak/Grik4^+	C57BL/6-Grik4^tm1(cre)Ksak	MGI:4360482
cn2	Grin2b^tm1Ksak/Grin2b^tm1Ksak Grik4^tm1(cre)Ksak/Grik4^+	C57BL/6-Grin2b^tm1Ksak Grik4^tm1(cre)Ksak	MGI:4360481

Genotype
MGI:4360482

ht1

• Allelic Composition: Grik4^tm1(cre)Ksak/Grik4⁺
• Genetic Background: C57BL/6-Grik4^tm1(cre)Ksak

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:152455 )

• mice are viable, fertile and do not display any overt behavioral or anotomic phenotype

Genotype
MGI:4360481

cn2

• Allelic Composition: Grin2b^tm1Ksak/Grin2b^tm1Ksak; Grik4^tm1(cre)Ksak/Grik4⁺
• Genetic Background: C57BL/6-Grin2b^tm1Ksak Grik4^tm1(cre)Ksak

nervous system

nervous system phenotype ( J:152455 )

N • normal brain structure and morphology

N • no overt behavioral phenotype

abnormal dendrite morphology ( J:152455 )

• reduced dendritic spine density

abnormal synapse morphology ( J:152455 )

• increased detergent solubility of GluN2A subunit and PSD-95 in mutant CA3

• decreased F-actin content

• reduced GluN1 subunit from the postsynaptic site

absence of NMDA-mediated synaptic currents ( J:152455 )

• NMDAR-mediated synaptic currents are abolished in three excitatory CA3 synapses: the commissural/associative-CA3 synapse, commissural-CA3 synapse, and mossy fiber-CA3 synapse

• reduced but detectable extrasynaptic NMDAR-mediated currents

reduced long-term potentiation ( J:152455 )

• LTP is abolished at the commissural/associative-CA3 synapse and commissural-CA3 synapse

• mossy fiber-CA3 synapse are normal