All Phenotypes Themis<tm1Lov> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Themis^tm1Lov/Themis^tm1Lov	involves: 129	MGI:4353387
cx2	H2-Ab1^b-tm1Gru/H2-Ab1^b-tm1Gru Themis^tm1Lov/Themis^tm1Lov	involves: 129	MGI:4353390
cx3	B2m^tm1Jae/B2m^tm1Jae Themis^tm1Lov/Themis^tm1Lov	involves: 129	MGI:4353391
cx4	Tg(CD2-Zbtb7b)C8Rbo/? Themis^tm1Lov/Themis^tm1Lov	involves: 129	MGI:4353392
cx5	Tg(TcraH-Y,TcrbH-Y)71Vbo/? Themis^tm1Lov/Themis^tm1Lov	involves: 129 * C57BL/6 * DBA/2	MGI:4353388
cx6	Tg(TcrAND)53Hed/? Themis^tm1Lov/Themis^tm1Lov	involves: 129 * C57BL/6 * SJL	MGI:4353389

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell differentiation ( J:151075 )

• the ratio of mature (CD24^lo) to immature (CD24^hi) TCR^hiCD4+ thymocytes is much lower than in controls indicating a defect in differentiation

abnormal negative T cell selection ( J:151075 )

• the number of transitional CD4+CD8^int thymocytes is lower indicating a defect in positive selection

decreased CD4-positive, alpha-beta T cell number ( J:151075 )

• peripheral CD4 T cells numbers are reduced by about 75%

decreased CD8-positive, alpha-beta T cell number ( J:151075 )

• peripheral CD8 T cell numbers are reduced by about a third

increased memory T cell number ( J:151075 )

• relative percentage of memory T cells is increased likely reflecting lymphopenia-driven population expansion

decreased regulatory T cell number ( J:151075 )

• CD4 regulatory T cell numbers are reduced in these mice

• the deletion of Vbeta8+ CD4SP thymocytes but not Vbeta8+ CD8SP thymocytes induced by the 'superantigen' staphylococcus enterotoxin B is impaired in these mice

decreased single-positive T cell number ( J:151075 )

• CD4 and CD8 single-positive thymocytes are reduced by a third to a half compared to controls

hematopoietic system

abnormal T cell differentiation ( J:151075 )

• the ratio of mature (CD24^lo) to immature (CD24^hi) TCR^hiCD4+ thymocytes is much lower than in controls indicating a defect in differentiation

abnormal negative T cell selection ( J:151075 )

• the number of transitional CD4+CD8^int thymocytes is lower indicating a defect in positive selection

decreased CD4-positive, alpha-beta T cell number ( J:151075 )

• peripheral CD4 T cells numbers are reduced by about 75%

decreased CD8-positive, alpha-beta T cell number ( J:151075 )

• peripheral CD8 T cell numbers are reduced by about a third

increased memory T cell number ( J:151075 )

• relative percentage of memory T cells is increased likely reflecting lymphopenia-driven population expansion

decreased regulatory T cell number ( J:151075 )

• CD4 regulatory T cell numbers are reduced in these mice

• the deletion of Vbeta8+ CD4SP thymocytes but not Vbeta8+ CD8SP thymocytes induced by the 'superantigen' staphylococcus enterotoxin B is impaired in these mice

decreased single-positive T cell number ( J:151075 )

• CD4 and CD8 single-positive thymocytes are reduced by a third to a half compared to controls

Allelic Composition	H2-Ab1^b-tm1Gru/H2-Ab1^b-tm1Gru Themis^tm1Lov/Themis^tm1Lov
Genetic Background	involves: 129

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-Ab1^b-tm1Gru mutation (11 available); any H2-Ab1 mutation (81 available)
Themis^tm1Lov mutation (0 available); any Themis mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal negative T cell selection ( J:151075 )

• the number of transitional CD4+CD8^int thymocytes is low in these mice when normally MHC-II deficient mice have high numbers of this population

hematopoietic system

abnormal negative T cell selection ( J:151075 )

• the number of transitional CD4+CD8^int thymocytes is low in these mice when normally MHC-II deficient mice have high numbers of this population

Allelic Composition	B2m^tm1Jae/B2m^tm1Jae Themis^tm1Lov/Themis^tm1Lov
Genetic Background	involves: 129

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2m^tm1Jae mutation (8 available); any B2m mutation (122 available)
Themis^tm1Lov mutation (0 available); any Themis mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:151075 )

• CD3^hi CD8 single-positive thymocytes are present in these mice

• these thymocytes are TCR^hiCD69⁺ which indicates that they received positively selecting signals by interaction with self peptide-MHC class II complexes but are subsequently 'redirected' to the CD8 lineage

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:151075 )

• CD3^hi CD8 single-positive thymocytes are present in these mice

Allelic Composition	Tg(CD2-Zbtb7b)C8Rbo/? Themis^tm1Lov/Themis^tm1Lov
Genetic Background	involves: 129

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(CD2-Zbtb7b)C8Rbo mutation (0 available)
Themis^tm1Lov mutation (0 available); any Themis mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell differentiation ( J:151075 )

• CD8 single positive thymocytes fail to develop in these mice

• transgene expression fails to alleviate the partial block in CD4 development

hematopoietic system

abnormal T cell differentiation ( J:151075 )

• CD8 single positive thymocytes fail to develop in these mice

• transgene expression fails to alleviate the partial block in CD4 development

Allelic Composition	Tg(TcraH-Y,TcrbH-Y)71Vbo/? Themis^tm1Lov/Themis^tm1Lov
Genetic Background	involves: 129 * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (3 available)
Themis^tm1Lov mutation (0 available); any Themis mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal negative T cell selection ( J:151075 )

• the percentage of DP thymocytes is consistently higher in male mice than in male transgenics on a wild-type background, which suggests that negative selection occurs less efficiently

abnormal positive T cell selection ( J:151075 )

• higher double positive thymocyte numbers and fewer single-positive thymoyctes in females indicate a defect in positive selection

increased double-positive T cell number ( J:151075 )

• double-positive thymocytes are increased in female mice

decreased single-positive T cell number ( J:151075 )

• CD8 single-positive thymocyte nubmers are reduced in these female mice

hematopoietic system

abnormal negative T cell selection ( J:151075 )

• the percentage of DP thymocytes is consistently higher in male mice than in male transgenics on a wild-type background, which suggests that negative selection occurs less efficiently

abnormal positive T cell selection ( J:151075 )

• higher double positive thymocyte numbers and fewer single-positive thymoyctes in females indicate a defect in positive selection

increased double-positive T cell number ( J:151075 )

• double-positive thymocytes are increased in female mice

decreased single-positive T cell number ( J:151075 )

• CD8 single-positive thymocyte nubmers are reduced in these female mice

Allelic Composition	Tg(TcrAND)53Hed/? Themis^tm1Lov/Themis^tm1Lov
Genetic Background	involves: 129 * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrAND)53Hed mutation (2 available)
Themis^tm1Lov mutation (0 available); any Themis mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:151075 )

• higher double positive thymocyte numbers and fewer single-positive thymoyctes indicate a defect in positive selection

increased double-positive T cell number ( J:151075 )

• double-positive thymocytes are increased in mice

decreased single-positive T cell number ( J:151075 )

• CD4 single-positive thymocyte numbers are reduced in these mice

hematopoietic system

abnormal positive T cell selection ( J:151075 )

• higher double positive thymocyte numbers and fewer single-positive thymoyctes indicate a defect in positive selection

increased double-positive T cell number ( J:151075 )

• double-positive thymocytes are increased in mice

decreased single-positive T cell number ( J:151075 )

• CD4 single-positive thymocyte numbers are reduced in these mice

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23