Phenotypes associated with this allele

• Allele Symbol: Nsmce2^{Gt(AA0032)Wtsi}
• Allele Name: gene trap AA0032, Wellcome Trust Sanger Institute
• Allele ID: MGI:4331032
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nsmce2^Gt(AA0032)Wtsi/Nsmce2^Gt(AA0032)Wtsi	involves: 129P2/OlaHsd * C57BL/6	MGI:5906750
ht2	Nsmce2^Gt(AA0032)Wtsi/Nsmce2^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5906752

Genotype
MGI:5906750

hm1

• Allelic Composition: Nsmce2^{Gt(AA0032)Wtsi}/Nsmce2^{Gt(AA0032)Wtsi}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation ( J:227197 )

• embryos are found at E2.5 but rapidly degenerate when cultured for 36 hours

cellular

abnormal cell mass ( J:227197 )

• E2.5 morulae contain cells of abnormal size suggesting imbalanced segregation

abnormal cell nucleus morphology ( J:227197 )

• cultured embryos accumulate cells with irregularly sized or multilobulated nuclei

abnormal chromosome morphology ( J:227197 )

• cultured embryos accumulate nuclei with condensed chromosomes

Genotype
MGI:5906752

ht2

• Allelic Composition: Nsmce2^{Gt(AA0032)Wtsi}/Nsmce2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

premature death ( J:227197 )

• reduced lifespan

neoplasm

increased tumor incidence ( J:227197 )

• increased incidence of multiple tumor types including; lymphoma, histiocytic sarcoma, pancreatic, hepatic and vascular

increased cardiovascular system tumor incidence ( J:227197 )

• increase in incidence of vascular tumors

increased pancreas tumor incidence ( J:227197 )

increased liver tumor incidence ( J:227197 )

increased lymphoma incidence ( J:227197 )

increased histiocytic sarcoma incidence ( J:227197 )

cellular

abnormal cell nucleus morphology ( J:227197 )

• increased incidence of micronucleated cells in cultured MEFs compared to wild-type cells

increased cell nucleus count ( J:227197 )

• increased incidence of polynucleated cells in cultured MEFs compared to wild-type cells

abnormal mitosis ( J:227197 )

• chromosome mis-segregation in MEFs

elevated level of mitotic sister chromatid exchange ( J:227197 )

• in MEFs

cardiovascular system

increased cardiovascular system tumor incidence ( J:227197 )

• increase in incidence of vascular tumors

endocrine/exocrine glands

increased pancreas tumor incidence ( J:227197 )

liver/biliary system

increased liver tumor incidence ( J:227197 )