Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggnbp2Gt(IST12476F10)Tigm mutation
(1 available);
any
Ggnbp2 mutation
(61 available)
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mortality/aging
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• homozyous mutant mice die between E13.5 and E15.5
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embryo
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N |
• average embryo sizes and placental weights in homozygous mutant fetuses were comparable to those of control siblings
• organization of the placental junctional zone, including primary trophoblast giant cell and spongiotrophoblast layers in homozygous null mutant placentae, was similar to controls
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• an anomalous labyrinth layer was noted in E13.5 and E15.5 placentae; abnormalities were indistinguishable at E10.5 but very pronounced at E15.5 dpc
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• the maternal and fetal vessel spaces in the labyrinth of mutant mice were taken up by large densely packed cell aggregates
• both maternal and fetal vascular spaces were dramatically reduced, while the labyrinth nonvessel area was markedly increased
• the labyrinth of mutant E15.5 placentae displayed an increase in the number of cells undergoing division, especially the cells in the densely packed cell aggregates; TUNEL assays did not exhibit a significant difference between Wt and mutant placentae so apoptosis was unaffected
• cell clusters likely were aggregates of trophoblast stem cells and trophoblast progenitors based on expression analysis
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integument
cardiovascular system
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• the maternal and fetal vessel spaces in the labyrinth of mutant mice were taken up by large densely packed cell aggregates
• both maternal and fetal vascular spaces were dramatically reduced, while the labyrinth nonvessel area was markedly increased
• the labyrinth of mutant E15.5 placentae displayed an increase in the number of cells undergoing division, especially the cells in the densely packed cell aggregates; TUNEL assays did not exhibit a significant difference between Wt and mutant placentae so apoptosis was unaffected
• cell clusters likely were aggregates of trophoblast stem cells and trophoblast progenitors based on expression analysis
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|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggnbp2Gt(IST12476F10)Tigm mutation
(1 available);
any
Ggnbp2 mutation
(61 available)
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mortality/aging
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• 6.8% of males and female mice are born alive and survive into adulthood
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reproductive system
 N |
• females are fertile
(J:249037)
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 N |
• males exhibit normal size of seminal vesicles and numbers of Leydig cells and Sertoli cells at 2 months of age
(J:249037)
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• acrosomes are distorted or detached
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• acrosomes are irregularly shaped
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• at 2 months of age, the number of spermatids per tubule is significantly reduced at each stage of the seminiferous epithelium cycle (I to XII) and at each step of spermatid development (1 to 16)
• both elongating and elongated spermatids show malformed nuclei/head shapes
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• microtubule bundles of the manchette skirt are unevenly distributed, with a shaggy appearance
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• in most spermatids, the manchette has lost its perinuclear symmetrical distribution and is dislocated
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• spermatid number is reduced at 2 months of age
• however, numbers of spermatogonia and spermatocytes are normal
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• no mature spermatozoa are found in the seminiferous tubules and epididymides at 2 months of age
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• lumens of the seminiferous tubules contain sloughed late steps of abnormal spermatids
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• seminiferous tubule epithelium contains disorganized elongated spermatids with deformed nuclei
• however, Sertoli cell numbers and integrity of the blood-testis barrier are normal
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• seminiferous tubules diameters are significantly reduced at 2 months of age
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• testes size is markedly smaller at 2 months of age
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• ratio of testis/body weight is significantly reduced at 2 months of age
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• at 1 month of age, testes show altered expression of several adhesion and associated proteins, suggesting that the adhesion integrity of the adlumenal germ epithelium is affected
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• spermatid numbers are markedly reduced at each stage of the seminiferous tubule cycle, with a more severe reduction observed at later steps of spermatid development
• round spermatids fail to develop into elongated spermatids
• multiple malformed elongating and elongated spermatids are observed, with abnormal formation and detachment of acrosomes, misshapen nuclei, ectopic manchettes, and cytoplasmic retention
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• only a few exfoliated germ cells, but no mature spermatozoa, are found in the lumens of epididymal ducts at 2 months of age
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• epididymis size is markedly smaller at 2 months of age
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• adult males are sterile
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cellular
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• acrosomes are distorted or detached
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• acrosomes are irregularly shaped
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• at 2 months of age, the number of spermatids per tubule is significantly reduced at each stage of the seminiferous epithelium cycle (I to XII) and at each step of spermatid development (1 to 16)
• both elongating and elongated spermatids show malformed nuclei/head shapes
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• microtubule bundles of the manchette skirt are unevenly distributed, with a shaggy appearance
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• in most spermatids, the manchette has lost its perinuclear symmetrical distribution and is dislocated
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• spermatid number is reduced at 2 months of age
• however, numbers of spermatogonia and spermatocytes are normal
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• no mature spermatozoa are found in the seminiferous tubules and epididymides at 2 months of age
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endocrine/exocrine glands
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• lumens of the seminiferous tubules contain sloughed late steps of abnormal spermatids
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• seminiferous tubule epithelium contains disorganized elongated spermatids with deformed nuclei
• however, Sertoli cell numbers and integrity of the blood-testis barrier are normal
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• seminiferous tubules diameters are significantly reduced at 2 months of age
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• testes size is markedly smaller at 2 months of age
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• ratio of testis/body weight is significantly reduced at 2 months of age
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• at 1 month of age, testes show altered expression of several adhesion and associated proteins, suggesting that the adhesion integrity of the adlumenal germ epithelium is affected
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homeostasis/metabolism
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