Phenotypes associated with this allele

• Allele Symbol: Prss16^tm1.1Mal
• Allele Name: targeted mutation 1.1, Bernard Malissen
• Allele ID: MGI:3843929
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prss16^tm1.1Mal/Prss16^tm1.1Mal	involves: 129S2/SvPas * C57BL/6	MGI:3843958
cx2	Prss16^tm1.1Mal/Prss16^tm1.1Mal Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcraH-Y,TcrbH-Y)1Pas/?	involves: 129S/SvEv * 129S2/SvPas * C57BL/6	MGI:3843959
cx3	Prss16^tm1.1Mal/Prss16^+ Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcraH-Y,TcrbH-Y)1Pas/?	involves: 129S/SvEv * 129S2/SvPas * C57BL/6	MGI:3843960

Genotype
MGI:3843958

hm1

• Allelic Composition: Prss16^tm1.1Mal/Prss16^tm1.1Mal
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal thymus cortex morphology ( J:148034 )

• CDR1-positive cortical thymic epithelial cells express 2-fold less MHC-II than controls

abnormal professional antigen presenting cell morphology ( J:148034 )

• CDR1-positive cortical thymic epithelial cells express 2-fold less MHC-II than controls

hematopoietic system

abnormal thymus cortex morphology ( J:148034 )

• CDR1-positive cortical thymic epithelial cells express 2-fold less MHC-II than controls

endocrine/exocrine glands

abnormal thymus cortex morphology ( J:148034 )

• CDR1-positive cortical thymic epithelial cells express 2-fold less MHC-II than controls

Genotype
MGI:3843959

cx2

• Allelic Composition: Prss16^tm1.1Mal/Prss16^tm1.1Mal; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraH-Y,TcrbH-Y)1Pas/?
• Genetic Background: involves: 129S/SvEv * 129S2/SvPas * C57BL/6

immune system

thymus hypoplasia ( J:148034 )

• thymus cellularity is reduced by more than half in female but not male mice compared to controls

abnormal positive T cell selection ( J:148034 )

• positive but not negative selection of MHC-II restricted T cells is impaired as evidenced by the low number of anti-HY (male antigen) CD4⁺T cells found in female mice

• when mice were irradiated and then reconstituted with bone marrow from OT-II mice, very few CD4⁺ T cells are produced

• when mice were irradiated and then reconstituted with bone marrow from OT-I mice, CD8⁺ T cells are produced at normal numbers

decreased double-positive T cell number ( J:148034 )

• the number of double-positive thymocytes is less than half in female but not male mice compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:148034 )

• there is about a 10-fold drop peripheral CD4 T cells for female but not male mice compared to controls

decreased single-positive T cell number ( J:148034 )

• there is a large decrease (greater than 5-fold) in CD4-single positive thymocytes that express the transgenic TCR in female mice

• CD4-single positive thymocytes expressing high levels of the transgenic TCR are decreased to an even greater extent

spleen hypoplasia ( J:148034 )

• spleen cellularity is reduced by about half in female but not male mice compared to controls

lymph node hypoplasia ( J:148034 )

• there is about a 10-fold drop in lymph node cellularity for female but not male mice compared to controls

hematopoietic system

thymus hypoplasia ( J:148034 )

• thymus cellularity is reduced by more than half in female but not male mice compared to controls

abnormal positive T cell selection ( J:148034 )

• positive but not negative selection of MHC-II restricted T cells is impaired as evidenced by the low number of anti-HY (male antigen) CD4⁺T cells found in female mice

• when mice were irradiated and then reconstituted with bone marrow from OT-II mice, very few CD4⁺ T cells are produced

• when mice were irradiated and then reconstituted with bone marrow from OT-I mice, CD8⁺ T cells are produced at normal numbers

decreased double-positive T cell number ( J:148034 )

• the number of double-positive thymocytes is less than half in female but not male mice compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:148034 )

• there is about a 10-fold drop peripheral CD4 T cells for female but not male mice compared to controls

decreased single-positive T cell number ( J:148034 )

• there is a large decrease (greater than 5-fold) in CD4-single positive thymocytes that express the transgenic TCR in female mice

• CD4-single positive thymocytes expressing high levels of the transgenic TCR are decreased to an even greater extent

spleen hypoplasia ( J:148034 )

• spleen cellularity is reduced by about half in female but not male mice compared to controls

endocrine/exocrine glands

thymus hypoplasia ( J:148034 )

• thymus cellularity is reduced by more than half in female but not male mice compared to controls

Genotype
MGI:3843960

cx3

• Allelic Composition: Prss16^tm1.1Mal/Prss16⁺; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraH-Y,TcrbH-Y)1Pas/?
• Genetic Background: involves: 129S/SvEv * 129S2/SvPas * C57BL/6

immune system

abnormal positive T cell selection ( J:148034 )

• positive but not negative selection is impaired as evidenced by the low number of anti-HY (male antigen) T cells found in female mice

decreased CD4-positive, alpha-beta T cell number ( J:148034 )

• there is about a 2-fold drop peripheral CD4 T cells for female but not male mice compared to controls

decreased single-positive T cell number ( J:148034 )

• there is a decrease (greater than 2-fold) in CD4-single positive thymocytes that express the transgenic TCR in female mice

lymph node hypoplasia ( J:148034 )

• there is about a 2-fold drop in lymph node cellularity for female but not male mice compared to controls

hematopoietic system

abnormal positive T cell selection ( J:148034 )

• positive but not negative selection is impaired as evidenced by the low number of anti-HY (male antigen) T cells found in female mice

decreased CD4-positive, alpha-beta T cell number ( J:148034 )

• there is about a 2-fold drop peripheral CD4 T cells for female but not male mice compared to controls

decreased single-positive T cell number ( J:148034 )

• there is a decrease (greater than 2-fold) in CD4-single positive thymocytes that express the transgenic TCR in female mice