Phenotypes associated with this allele

• Allele Symbol: Esr1^tm1.2Awo
• Allele Name: targeted mutation 1.2, Andrew Wolfe
• Allele ID: MGI:3842664
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Esr1^tm1.2Awo/Esr1^tm1.2Awo Kiss1^tm1.1(cre)Uboe/Kiss1^+	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:4878879
cn2	Esr1^tm1.2Awo/Esr1^tm1.2Awo Tg(Cga-cre)1Awo/0	involves: C57BL/6 * SJL	MGI:4360656

Genotype
MGI:4878879

cn1

• Allelic Composition: Esr1^tm1.2Awo/Esr1^tm1.2Awo; Kiss1^{tm1.1(cre)Uboe}/Kiss1⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:167285 )

♂N • testis weight in adult males is not different from wild-type

absent corpus luteum ( J:167285 )

♀ • in P35 ovaries, corpora lutea are completely absent

♀ • in adult ovaries, few or no corpora lutea are observed

ovary cyst ( J:167285 )

♀ • ovaries from P25 and P35 animals contain numerous cysts

ovarian follicular cyst ( J:167285 )

♀ • adult ovaries also show numerous large follicular cysts

early vaginal opening ( J:167285 )

♀ • observed significantly earlier than in wild-type (13 vs 29 days postnatal)

abnormal estrous cycle ( J:167285 )

♀ • females exhibit prolonged period (weeks or months) of persistent vaginal cornification

♀ • in some animals, isolated "breakthrough" cycles occur followed by more periods of prolonged acyclicity

prolonged diestrus ( J:167285 )

♀ • prolonged period of persistent vaginal cornification is followed by persistent diestrus state

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:167285 )

N • serum luteinizing hormone (LH) levels in adult castrated (cx) males is not different from cx wil-type males; at P35 serum LH levels are similar in mutant and wild-type males

decreased circulating luteinizing hormone level ( J:167285 )

• in absence of ovarian restraint (after ovariectomy), LH secretion is greatly reduced in adult females compared to ovariectomized wild-type females

increased circulating luteinizing hormone level ( J:167285 )

• serum LH concentration is 6-7-fold higher at P15 than in age-matched wild-type; levels remain elevated at P25, then decline to wild-type levels by P35

endocrine/exocrine glands

absent corpus luteum ( J:167285 )

♀ • in P35 ovaries, corpora lutea are completely absent

♀ • in adult ovaries, few or no corpora lutea are observed

ovary cyst ( J:167285 )

♀ • ovaries from P25 and P35 animals contain numerous cysts

ovarian follicular cyst ( J:167285 )

♀ • adult ovaries also show numerous large follicular cysts

growth/size/body

ovary cyst ( J:167285 )

♀ • ovaries from P25 and P35 animals contain numerous cysts

ovarian follicular cyst ( J:167285 )

♀ • adult ovaries also show numerous large follicular cysts

Genotype
MGI:4360656

cn2

• Allelic Composition: Esr1^tm1.2Awo/Esr1^tm1.2Awo; Tg(Cga-cre)1Awo/0
• Genetic Background: involves: C57BL/6 * SJL

reproductive system

reproductive system phenotype ( J:152925 )

N • male mice exhibit normal fertility

absent corpus luteum ( J:152925 )

♀ • in infertile mice

decreased corpora lutea number ( J:152925 )

♀ • in mice with decreased fertility

abnormal ovarian follicle morphology ( J:152925 )

♀ • atretic follicles and primary and secondary follicles fail to reach the periphery of the ovary unlike in wild-type mice

abnormal estrous cycle ( J:152925 )

♀

female infertility ( J:152925 )

♀ • despite the presence of vaginal plus, 5 of 9 mice are infertile unlike wild-type mice

reduced female fertility ( J:152925 )

♀ • 4 of 9 mice

decreased litter size ( J:152925 )

endocrine/exocrine glands

decreased gonadotroph cell number ( J:152925 )

absent corpus luteum ( J:152925 )

♀ • in infertile mice

decreased corpora lutea number ( J:152925 )

♀ • in mice with decreased fertility

abnormal ovarian follicle morphology ( J:152925 )

♀ • atretic follicles and primary and secondary follicles fail to reach the periphery of the ovary unlike in wild-type mice

homeostasis/metabolism

increased circulating estradiol level ( J:152925 )

abnormal luteinizing hormone level ( J:152925 )

• luteinizing hormone basal levels are higher than in wild-type mice, values fail to surge with the estrous cycle, and peak levels are 18-fold lower than in wild-type mice

decreased circulating luteinizing hormone level ( J:152925 )

• peak levels are 18-fold lower than in wild-type mice

increased circulating luteinizing hormone level ( J:152925 )

• basal luteinizing hormone serum levels are 4-fold higher than in wild-type mice

nervous system

decreased gonadotroph cell number ( J:152925 )