Phenotypes associated with this allele

• Allele Symbol: Btla^tm1Dgra
• Allele Name: targeted mutation 1, Daniel Graf
• Allele ID: MGI:3842627
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Btla^tm1Dgra/Btla^tm1Dgra	involves: C57BL/6	MGI:3842630
cx2	Btla^tm1Dgra/Btla^tm1Dgra Tg(TcraTcrb)425Cbn/?	involves: C57BL/6	MGI:3842632
cx3	Btla^tm1Dgra/Btla^tm1Dgra Tg(TcraTcrb)1100Mjb/?	involves: C57BL/6	MGI:3842633

Genotype
MGI:3842630

hm1

• Allelic Composition: Btla^tm1Dgra/Btla^tm1Dgra
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell activation ( J:147751 )

• T cells show a heightened response to anti-CD3 stimulation

abnormal peripheral T cell anergy ( J:147751 )

• splenocytes are resistant to oral tolerance

• T cells from mutant mice fed OVA peptide produce higher levels of IL-2 and IFN-gamma, and proliferate more vigorously in response to OVA restimulation than do controls

hematopoietic system

abnormal T cell activation ( J:147751 )

• T cells show a heightened response to anti-CD3 stimulation

Genotype
MGI:3842632

cx2

• Allelic Composition: Btla^tm1Dgra/Btla^tm1Dgra; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: C57BL/6

immune system

abnormal peripheral T cell anergy ( J:147751 )

• T cells are resistant to tolerance induced by peptide injections

• T cells from mutant mice pre-treated with OVA produce higher levels of IL-2 and IFN-gamma, and proliferate more vigorously in response to OVA restimulation than do controls

• IFN-gamma production is higher for T cells from mutant mice pre-treated with OVA compared to mutant mice pre-treated with PBS

Genotype
MGI:3842633

cx3

• Allelic Composition: Btla^tm1Dgra/Btla^tm1Dgra; Tg(TcraTcrb)1100Mjb/?
• Genetic Background: involves: C57BL/6

immune system

abnormal peripheral T cell anergy ( J:147751 )

• CD8⁺ T cells from these mutant mice induce diabetes as early as 6 days after transfer into Tg(Ins2-TFRC/OVA)296Wehi transgenic mice that express OVA in insulin-producing cells

• 60% of transgenic mice develop diabetes and almost all mice have invasive insulitis compared to transgenic mice receiving wild-type OT-I cells where 60% of mice display no disease

• mutant OT-I T cells found in the pancreatic lymph nodes have twice as much proliferation as controls 50 hours after transfer compared to transferred wild-type OT-I T cells

• two weeks after transfer, there are twice as many mutant OT-I T cells in pancreatic lymph nodes compared to wild-type OT-I T cells controls

• thus, mutant autoreactive OT-I CD8⁺ T cells are resistant to tolerance induction mechanisms that limit the expansion of autoreactive CD8 ⁺ T cells