Phenotypes associated with this allele

• Allele Symbol: Sgpl1^{Gt(OST58278)Lex}
• Allele Name: gene trap OST58278, Lexicon Genetics
• Allele ID: MGI:3833931
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sgpl1^Gt(OST58278)Lex/Sgpl1^Gt(OST58278)Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:3833997
ht2	Sgpl1^Gt(OST58278)Lex/Sgpl1^tm1(SGPL1)Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:3833999

Genotype
MGI:3833997

hm1

• Allelic Composition: Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{Gt(OST58278)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Thymus abnormalities in Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{Gt(OST58278)Lex}, Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{tm1(SGPL1)Lex} and Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} mice

mortality/aging

premature death ( J:145159 )

• mice die by 15 weeks of age

immune system

thymus cortex hypoplasia ( J:145159 )

thymus medulla hyperplasia ( J:145159 )

• hypercellular

thymus hypoplasia ( J:145159 )

• 10% of wild-type

abnormal lymphopoiesis ( J:145159 )

• the development and recirculation of lymphocytes, especially T differentiation and egress, are disrupted

decreased neutrophil cell number ( J:145159 )

increased granulocyte number ( J:145159 )

• in the lymph nodes, bone marrow, and lamina propria of the small intestine

increased CD4-positive, alpha-beta T cell number ( J:145159 )

• 5-fold in the thymus

increased CD8-positive, alpha-beta T cell number ( J:145159 )

• 5-fold in the thymus

decreased lymphocyte cell number ( J:145159 )

• in the lymph nodes and lamina propria of the small intestine

decreased B cell number ( J:145159 )

• 41% of wild-type in the spleen

decreased pre-B cell number ( J:145159 )

• the fraction of pre-B cells in the bone marrow is decreased compared to in wild-type mice while pro-B cell and mature B cell frequencies are normal

decreased T cell number ( J:145159 )

• peripheral T cells are almost completely absent

decreased double-positive T cell number ( J:145159 )

• without an increased in double negative cells

increased monocyte cell number ( J:145159 )

abnormal osteoclast morphology ( J:145159 )

• osteoclasts are distended by an abundant pale and granular eosinophilic cytoplasm that displaces the nuclei and have less direct contact with the bone surfaces compared to wild-type cells

increased osteoclast cell number ( J:145159 )

spleen hypoplasia ( J:145159 )

• 47% of wild-type

abnormal spleen periarteriolar lymphoid sheath morphology ( J:145159 )

• the arrangement of the periarteriolar lymphoid sheaths and lymphoid follicles is abnormal, and T cells are severely depleted compared to in wild-type mice

• however, B cell areas are normal

• occasional clusters of plasma cells are observed unlike in wild-type mice

abnormal lymph node morphology ( J:145159 )

• lymph nodes contain smaller B cell follicles and reduced numbers of lymphocytes in the paracortical zone with increased numbers of granulocytes and macrophages compared to in wild-type mice

lymph node hypoplasia ( J:145159 )

• 57% of wild-type

lymphoid hypoplasia ( J:145159 )

respiratory system

abnormal pulmonary alveolus morphology ( J:145159 )

• pulmonary alveoli contain proteinaceous material unlike in wild-type mice

thick pulmonary interalveolar septum ( J:145159 )

• due to cellular hypertrophy and collagen deposition

skeleton

abnormal osteoclast morphology ( J:145159 )

• osteoclasts are distended by an abundant pale and granular eosinophilic cytoplasm that displaces the nuclei and have less direct contact with the bone surfaces compared to wild-type cells

increased osteoclast cell number ( J:145159 )

abnormal bone marrow morphology ( J:145159 )

• hypocellular

abnormal trabecular bone morphology ( J:145159 )

• the volume and extent of trabecular bone is increased in the sternebra and long bones compared to in wild-type mice

cardiovascular system

abnormal heart morphology ( J:145159 )

• heart lesions have patchy to diffuse expansion of the interstitium by vacuoles and vacuolated mesenchymal cells unlike in wild-type mice

abnormal myocardial fiber morphology ( J:145159 )

• some have enlarged vesicular nuclei unlike in wild-type mice

digestive/alimentary system

abnormal small intestine morphology ( J:145159 )

• the numbers of lymphocytes are reduced while the numbers of granulocytes are increased in the lamina propria of the small intestine compared to in wild-type mice

growth/size/body

decreased body weight ( J:145159 )

• at 4 weeks

postnatal growth retardation ( J:145159 )

• mice fail to thrive in the first weeks of life

renal/urinary system

abnormal urothelium morphology ( J:145159 )

• mice exhibit lesions of the uroepithelium affecting the renal pelvis, ureters, and urinary bladder and characterized by ballooning vacuolization, degeneration, and apoptosis of urothelial cells unlike in wild-type mice

• lesions are more severe in the superficial umbrella cell layer

muscle

abnormal myocardial fiber morphology ( J:145159 )

• some have enlarged vesicular nuclei unlike in wild-type mice

hematopoietic system

thymus cortex hypoplasia ( J:145159 )

thymus medulla hyperplasia ( J:145159 )

• hypercellular

thymus hypoplasia ( J:145159 )

• 10% of wild-type

abnormal definitive hematopoiesis ( J:145159 )

• hematopoiesis in the red pulp is increased compared to in wild-type mice

abnormal lymphopoiesis ( J:145159 )

• the development and recirculation of lymphocytes, especially T differentiation and egress, are disrupted

decreased neutrophil cell number ( J:145159 )

increased granulocyte number ( J:145159 )

• in the lymph nodes, bone marrow, and lamina propria of the small intestine

increased CD4-positive, alpha-beta T cell number ( J:145159 )

• 5-fold in the thymus

increased CD8-positive, alpha-beta T cell number ( J:145159 )

• 5-fold in the thymus

decreased lymphocyte cell number ( J:145159 )

• in the lymph nodes and lamina propria of the small intestine

decreased B cell number ( J:145159 )

• 41% of wild-type in the spleen

decreased pre-B cell number ( J:145159 )

• the fraction of pre-B cells in the bone marrow is decreased compared to in wild-type mice while pro-B cell and mature B cell frequencies are normal

decreased T cell number ( J:145159 )

• peripheral T cells are almost completely absent

decreased double-positive T cell number ( J:145159 )

• without an increased in double negative cells

increased monocyte cell number ( J:145159 )

abnormal osteoclast morphology ( J:145159 )

• osteoclasts are distended by an abundant pale and granular eosinophilic cytoplasm that displaces the nuclei and have less direct contact with the bone surfaces compared to wild-type cells

increased osteoclast cell number ( J:145159 )

spleen hypoplasia ( J:145159 )

• 47% of wild-type

abnormal spleen periarteriolar lymphoid sheath morphology ( J:145159 )

• the arrangement of the periarteriolar lymphoid sheaths and lymphoid follicles is abnormal, and T cells are severely depleted compared to in wild-type mice

• however, B cell areas are normal

• occasional clusters of plasma cells are observed unlike in wild-type mice

endocrine/exocrine glands

thymus cortex hypoplasia ( J:145159 )

thymus medulla hyperplasia ( J:145159 )

• hypercellular

thymus hypoplasia ( J:145159 )

• 10% of wild-type

Genotype
MGI:3833999

ht2

• Allelic Composition: Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{tm1(SGPL1)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

Thymus abnormalities in Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{Gt(OST58278)Lex}, Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{tm1(SGPL1)Lex} and Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} mice

immune system

decreased T cell number ( J:145159 )

• mice have fewer circulating T cells than in Sgpl1^{tm1(SGPL1)Lex} homozygotes

abnormal osteoclast morphology ( J:145159 )

• a small number of distended osteoclasts are present at the growth plate

• however, mice exhibit normal trabecular bone in the meptaphysis and diaphysis of the long bones

abnormal immune system organ morphology ( J:145159 )

• mice exhibit the same defects in lymphoid tissue morphology as in Sgpl1^{tm1(SGPL1)Lex} homozygotes

• however, mice do not exhibit defects in non-lymphoid tissues

thymus cortex hypoplasia ( J:145159 )

• the number of lymphocytes in the thymic cortex is mildly decreased compared to in wild-type mice

thymus medulla hyperplasia ( J:145159 )

• the number of lymphocytes in the medulla is mildly increased compared to in wild-type mice

renal/urinary system

abnormal urinary bladder urothelium morphology ( J:145159 )

• mice exhibit multifocal minimal vacuolization and degeneration of the urinary bladder urothelium unlike in wild-type mice

skeleton

abnormal osteoclast morphology ( J:145159 )

• a small number of distended osteoclasts are present at the growth plate

• however, mice exhibit normal trabecular bone in the meptaphysis and diaphysis of the long bones

cardiovascular system

cardiovascular system phenotype ( J:145159 )

N • heart morphology is normal

respiratory system

respiratory system phenotype ( J:145159 )

N • lung morphology is normal

hematopoietic system

thymus cortex hypoplasia ( J:145159 )

• the number of lymphocytes in the thymic cortex is mildly decreased compared to in wild-type mice

thymus medulla hyperplasia ( J:145159 )

• the number of lymphocytes in the medulla is mildly increased compared to in wild-type mice

decreased T cell number ( J:145159 )

• mice have fewer circulating T cells than in Sgpl1^{tm1(SGPL1)Lex} homozygotes

abnormal osteoclast morphology ( J:145159 )

• a small number of distended osteoclasts are present at the growth plate

• however, mice exhibit normal trabecular bone in the meptaphysis and diaphysis of the long bones

endocrine/exocrine glands

thymus cortex hypoplasia ( J:145159 )

• the number of lymphocytes in the thymic cortex is mildly decreased compared to in wild-type mice

thymus medulla hyperplasia ( J:145159 )

• the number of lymphocytes in the medulla is mildly increased compared to in wild-type mice