Phenotypes associated with this allele

• Allele Symbol: Plin2^tm1Itl
• Allele Name: targeted mutation 1, inGenious Targeting Laboratory
• Allele ID: MGI:3820655
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plin2^tm1Itl/Plin2^tm1Itl	involves: 129S6/SvEvTac * BALB/c	MGI:3820657
hm2	Plin2^tm1Itl/Plin2^tm1Itl	involves: 129S6/SvEvTac * C57BL/6	MGI:3820658
cx3	Plin2^tm1Itl/Plin2^tm1Itl Rpe65^tm1Tmr/Rpe65^tm1Tmr Tyr^c-2J/Tyr^c-2J	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * BALB/c * C57BL/6	MGI:3820659

Genotype
MGI:3820657

hm1

• Allelic Composition: Plin2^tm1Itl/Plin2^tm1Itl
• Genetic Background: involves: 129S6/SvEvTac * BALB/c

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

vision/eye phenotype ( J:142021 )

N • despite disruptions in eye physiology, eye morphology is normal

abnormal eye electrophysiology ( J:142021 )

• at 1 year of age, mice exhibit attenuated electroretinogram responses compared to wild-type mice affecting both a- and b-waves under scotopic conditions and b-waves under photopic conditions

delayed dark adaptation ( J:142021 )

• Background Sensitivity: delayed dark adaptation is more prominent on a mixed BALB/c background then on a mixed C57BL/6 background

homeostasis/metabolism

abnormal vitamin A metabolism ( J:142021 )

• under dark-adapted conditions, mice exhibit a decrease in all-trans-retinal but slower clearance of all-trans retinal and all-trans retinol compared to in wild-type mice results in a 20% increase in all-trans-retinal levels immediately after light illumination and a 40% increase 15 minutes after illumination

• newly formed retinyl esters assemble into more aggregates than in wild-type mice and these aggregates are highly mobile unlike in wild-type cells

• however, accumulation of A2E and iso-A2E is normal

Genotype
MGI:3820658

hm2

• Allelic Composition: Plin2^tm1Itl/Plin2^tm1Itl
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

vision/eye

delayed dark adaptation ( J:142021 )

• Background Sensitivity: delayed dark adaptation is less prominent on a mixed C57BL/6 background then on a mixed BALB/c background

Genotype
MGI:3820659

cx3

• Allelic Composition: Plin2^tm1Itl/Plin2^tm1Itl; Rpe65^tm1Tmr/Rpe65^tm1Tmr; Tyr^c-2J/Tyr^c-2J
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * BALB/c * C57BL/6

homeostasis/metabolism

abnormal vitamin A metabolism ( J:142021 )

• at 3 to 4 months, retinyl ester accumulation is 70% of the accumulation observed in Rpe65^tm1Tmr homozygotes