Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mecp2tm1.1Bird mutation
(1 available);
any
Mecp2 mutation
(38 available)
Tg(MECP2)1Hzo mutation
(1 available)
|
|
|
mortality/aging
 N |
• premature death in male Mecp2-null mice at 10-11 weeks is rescued
|
behavior/neurological
|
N |
• neurological abnormalities seen in single transgenic mutant are rescued by loss of Mecp2
|
nervous system
|
N |
• amplitudes of EPSCs, RRP size, and mEPSC frequency, amplitude, and decay kinetics are normalized in double mutant brains compared to either single mutant brain
• glutamatergic synaptic density is normalized in double mutant brains compared to either single mutant brain
|
Allelic
Composition |
Crhtm1Maj/Crh+
Tg(MECP2)1Hzo/0
|
|
Genetic
Background |
involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6 * FVB/N |
|
|
|
behavior/neurological
|
|
• anxiety-like behavior is reduced compared to single Tg(MECP2)1Hzo hemizygous mice, with double mutants being less anxious in the elevated plus maze and the light/dark box
|
homeostasis/metabolism
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Oprm1tm1Kff mutation
(1 available);
any
Oprm1 mutation
(53 available)
Tg(MECP2)1Hzo mutation
(1 available)
|
|
|
behavior/neurological
|
|
• double mutants exhibit a similar level of anxiety-like behavior in the elevated plus maze and light/dark box as single Tg(MECP2)1Hzo hemizygous mice
• however, double mutants exhibit improved social behavior deficits compared to single Tg(MECP2)1Hzo mice, spending more time investigating both familiar and novel partners
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Crhr1tm1Klee mutation
(1 available);
any
Crhr1 mutation
(26 available)
Tg(MECP2)1Hzo mutation
(1 available)
|
|
|
behavior/neurological
|
|
• anxiety-like behavior is reduced compared to single Tg(MECP2)1Hzo hemizygous mice, with double mutants being less anxious in the elevated plus maze and the light/dark box
|
Allelic
Composition |
Tg(MECP2)1Hzo/0
|
|
Genetic
Background |
either: (FVB/N x 129S6/SvEvTac)F1 or (FVB/N x C57BL/6J)F1 |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(MECP2)1Hzo mutation
(1 available)
|
|
|
behavior/neurological
|
|
• mutants exhibit anxiety-like behavior in the elevated plus maze
• mutants spend less time in the lit compartment of the light/dark box compared to wild-type mice indicating anxiety-like behavior
|
|
|
• mutants are less interested in their familiar or novel partner mice in the partition test for social interaction and recognition
• in the three-chamber test for sociability, mutants show a deficit in social approach behavior toward novel partner mice, without a deficit in interest toward a novel object or a deficit in activity or preference for either chamber
|
homeostasis/metabolism
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(MECP2)1Hzo mutation
(1 available)
|
|
|
vision/eye
|
|
• V1 neurons show age-dependent change in firing rate, with higher evoked responses at 8 weeks but lower at 14 weeks and lower firing rates to low and high spatial frequencies in 14 week old V1 neurons
|
|
|
• primary visual cortex (V1) neurons show a preference for higher spatial frequencies at 8 and 14 weeks of age indicating higher visual acuity
• in a two-alternative forced choice visual detection task, mice exhibit higher behavioral performance in spatial frequency detection
|
|
|
• V1 neurons show age-dependent (at 8 weeks but not 14 weeks of age) change in contrast sensitivity, indicating higher contrast sensitivity
• behavioral performance in visual detection of stimuli that varies in contrast shows improved performance in detecting low contrasts
|
Allelic
Composition |
Tg(MECP2)1Hzo/0
|
|
Genetic
Background |
involves: FVB |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(MECP2)1Hzo mutation
(1 available)
|
|
|
mortality/aging
|
|
• 30% of mice die between 20 weeks and 1 year of age; mice surviving past 1 year of age have a normal lifespan
|
nervous system
|
N |
• no alteration in synaptic release probability is detected in neurons
|
|
|
• visually observed as early as 20 weeks of age, with increased frequency by 50 weeks
|
|
|
• in vitro, density of synaptic markers is increased by 60%
• in vivo, at 2 weeks postnatal, number of glutamatergic synapses is increased by 80%; by 5 weeks, difference is much smaller but is still slightly significant
|
|
|
• neurons exhibit a 104% enhancement in RRP charge relative to wild-type
|
|
|
• evoked EPSC amplitudes show a 116% enhancement compared to wild-type
|
|
|
• robust enhancement is observed in mutants
|
|
|
• mEPSCs show a significant increase in frequency compared to wild-type
|
|
|
• PPF is greatly enhanced in transgenic mice
|
behavior/neurological
|
|
• in conditioned fear analysis, mutants and wild-type show similar results in cued fear conditioning, but in contextual conditioning, mutants show elevated freezing behavior indicating greater hippocampal learning
|
|
|
• increased propensity to bite
|
|
|
• mice show lower anxiety evidenced by increased vertical activity in open field test
|
|
|
• forepaw clasping when lifted by tail
|
|
|
• in dowel test, most animals remain inactive on wooden rod the entire trial period
|
|
|
• at 10 weeks of age, transgenics perform better on a rotating rod test on day 2 of training relative to wild-type; upon retesting at 20 weeks, mutants perform significantly better than wild-type
|
|
|
• mice show increased vertical activity compared to controls at 10 and 20 weeks
|
|
|
• in dowel test, transgenic animals display significantly reduced movement and walk off the length of an elevated dowel fewer times than wild-type
• characterized by freezing behavior
|
|
|
• visually observed as early as 20 weeks of age, with increased frequency by 50 weeks
|
skeleton
Analysis Tools