All Phenotypes Tg(Lck-Akt1*)M-1Pnt MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(Lck-Akt1*)M-1Pnt/0	involves: 129S/SvEv * C3H/He * C57BL/6	MGI:3803973
tg2	Tg(Lck-Akt1*)M-1Pnt/0	involves: C3H/He * C57BL/6	MGI:3803812

Allelic Composition	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(Lck-Akt1*)M-1Pnt/0
Genetic Background	involves: 129S/SvEv * C3H/He * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2^tm1Fwa mutation (48 available); any Rag2 mutation (117 available)
Tg(Lck-Akt1*)M-1Pnt mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased double-positive T cell number ( J:135903 )

• 96% of thymocytes are double positive

• the presence of the transgene rescues the block of T cell development that occurs at DN3 in Rag2^tm1Fwa homozygotes

absent CD4-positive, alpha-beta T cells ( J:135903 )

• despite the presence of double positive thymocytes, single positive T cells do not develop

absent CD8-positive, alpha-beta T cells ( J:135903 )

• despite the presence of double positive thymocytes, single positive T cells do not develop

hematopoietic system

increased double-positive T cell number ( J:135903 )

• 96% of thymocytes are double positive

• the presence of the transgene rescues the block of T cell development that occurs at DN3 in Rag2^tm1Fwa homozygotes

absent CD4-positive, alpha-beta T cells ( J:135903 )

• despite the presence of double positive thymocytes, single positive T cells do not develop

absent CD8-positive, alpha-beta T cells ( J:135903 )

• despite the presence of double positive thymocytes, single positive T cells do not develop

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased survivor rate ( J:73487 )

• about half of the mice are dead at 90 days of age from thymic lymphomas

• less than 20% of the mice live past 240 days

neoplasm

increased T cell derived lymphoma incidence ( J:73487 )

• most mice die of thymic lymphomas

• lymphomas contain clonal rearrangements in the T cell receptor genes

• all tumors are lymphoblastic lymphomas with variable expression of the CD4 and CD8 genes

immune system

decreased thymocyte number ( J:73487 )

• number of thymocytes is decreased by more than 20% compared to wild-type mice despite the thymus having similar weight as in wild-type

enlarged spleen ( J:73487 )

• is often observed in necropsy due to lymphomas

increased double-negative T cell number ( J:135903 )

• there is a five-fold increase in the percentage of double negative thymocytes found in the thymus

• double negative thymocytes progress rapidly through the DN3 to DN4 stage

increased single-positive T cell number ( J:135903 )

• the percentage of single positive thymocytes is increased in these mice

cellular

abnormal cell cycle checkpoint function ( J:73487 )

• thymocyte cell size is significantly increased in these mice

• thymocytes express high levels of cyclin D2, D3,and E and promote progression through G1

hematopoietic system

decreased thymocyte number ( J:73487 )

• number of thymocytes is decreased by more than 20% compared to wild-type mice despite the thymus having similar weight as in wild-type

enlarged spleen ( J:73487 )

• is often observed in necropsy due to lymphomas

increased double-negative T cell number ( J:135903 )

• there is a five-fold increase in the percentage of double negative thymocytes found in the thymus

• double negative thymocytes progress rapidly through the DN3 to DN4 stage

increased single-positive T cell number ( J:135903 )

• the percentage of single positive thymocytes is increased in these mice

endocrine/exocrine glands

decreased thymocyte number ( J:73487 )

• number of thymocytes is decreased by more than 20% compared to wild-type mice despite the thymus having similar weight as in wild-type

increased T cell derived lymphoma incidence ( J:73487 )

• most mice die of thymic lymphomas

• lymphomas contain clonal rearrangements in the T cell receptor genes

• all tumors are lymphoblastic lymphomas with variable expression of the CD4 and CD8 genes

growth/size/body

enlarged spleen ( J:73487 )

• is often observed in necropsy due to lymphomas

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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