Phenotypes associated with this allele

• Allele Symbol: Wt1^{tm2(cre/ERT2)Wtp}
• Allele Name: targeted mutation 2, William T Pu
• Allele ID: MGI:3801682
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Drosha^tm1Litt/Drosha^tm1Litt Wt1^tm2(cre/ERT2)Wtp/Wt1^+	involves: 129P2/OlaHsd * 129S4/SvJae	MGI:6887989
cn2	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^tm3(tetO-Mirlet7g/Mir21)Gqda Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Wt1^tm2(cre/ERT2)Wtp/Wt1^+	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6	MGI:5638880
cn3	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Wt1^tm2(cre/ERT2)Wtp/Wt1^+	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6	MGI:5638793

Genotype
MGI:6887989

cn1

• Allelic Composition: Drosha^tm1Litt/Drosha^tm1Litt; Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

renal/urinary system phenotype ( J:321428 )

N • normal kidney size with fully developed glomeruli

increased urine protein level ( J:321428 )

• protein deposits in kidney tubules by age P28

homeostasis/metabolism

increased urine protein level ( J:321428 )

• protein deposits in kidney tubules by age P28

neoplasm

neoplasm ( J:321428 )

N • no kidney tumor development in mice up to 6 months old

mortality/aging

mortality/aging ( J:321428 )

N • viable; live to at least 6 months

Genotype
MGI:5638880

cn2

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda}; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6

renal/urinary system

small kidney ( J:211179 )

• small kidney in mice induced with doxycycline from E14.5 until the end of the experiment

• however, cap mesenchyme differentiates normally in doxycycline induced mutants

Genotype
MGI:5638793

cn3

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6

neoplasm

increased kidney tumor incidence ( J:211179 )

• all mice develop kidney tumors within the first 2 weeks of life following doxycycline (Dox) induction during embryonic development at E0, E14.5 or E18.5

• tumors resemble Wilms tumor

• mice treated with doxycycline at P10 do not develop tumors

renal/urinary system

kidney cyst ( J:211179 )

• mice treated with doxycycline at P10 develop cystic kidneys

increased kidney tumor incidence ( J:211179 )

• all mice develop kidney tumors within the first 2 weeks of life following doxycycline (Dox) induction during embryonic development at E0, E14.5 or E18.5

• tumors resemble Wilms tumor

• mice treated with doxycycline at P10 do not develop tumors

abnormal kidney mesenchyme morphology ( J:211179 )

• doxycycline induced mice exhibit persistent proliferation of cap mesenchyme cells in adults

• however, cap mesenchyme cells within tumors retain a differentiation capacity that recapitulates normal kidney development

delayed kidney development ( J:211179 )

• timing of kidney development is prolonged in doxycycline induced mice, with sustaining proliferation of the cap mesenchyme cells into adulthood

growth/size/body

kidney cyst ( J:211179 )

• mice treated with doxycycline at P10 develop cystic kidneys

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
nephroblastoma		DOID:2154	OMIM:194070	J:211179