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Allele Symbol Allele Name Allele ID |
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde targeted mutation 1, Michael D Ehlers MGI:3796847 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• capsaicin infusion after focal induction of Trpv1 expression in subsets of striatal neurons by injection of a lentiviral-cre vector recapitulates contralateral circling behavior at one week post-injection; circling subsides within 15 minutes of capsaicin application
• ipsilateral circling is not observed after capsaicin infusion
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• cheek injection of capsaicin does not evoke pain behavior (forepaw facial wiping), in contrast to robust facial wiping observed in treated wild-type
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• mice have impaired nociceptive responses, showing increased latencies in tail-immersion and hot plate paradigms
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• robust itch behavior is observed after cheek injection of capsaicin compared to little hindpaw scratching seen in treated wild-type
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• capsaicin infusion into striatum does not affect exploratory or motor behavior in Cre-activated mutants or controls
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• infusion of capsaicin into striatum of results in stereotyped contralateral circling behavior within 5 minutes of delivery, while control mice without activation of Trpv1 show no effect
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• infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
• at doses sufficient to induce circling, no cell death is observed
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• in layer 5 cortical slices, capsaicin induces action potentials while cells from Cre-negative animals show no response
• neurons in slices show dose-dependent depolarizing currents in response to capsaicin applied to the bath
• capsaicin applied acutely to single cells causes reproducible bursts of action potentials with increases in firing rate; frequency of firing is dependent on duration and concentration of capsaicin application
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• infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
• at doses sufficient to induce circling, no cell death is observed
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• infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
• at doses sufficient to induce circling, no cell death is observed
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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