Phenotypes associated with this allele

• Allele Symbol: Tg(Fev-flpe)1Dym
• Allele Name: transgene insertion 1, Susan Dymecki
• Allele ID: MGI:3795206
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ En1^tm2(cre)Wrst/En1^+ Tg(Fev-flpe)1Dym/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:4412291
cn2	En1^tm2(cre)Wrst/? Gt(ROSA)26Sor^tm5(CAG-EGFP,-lacZ)Dym/? Tg(Fev-flpe)1Dym/?	involves: 129S1/Sv * 129X1/SvJ	MGI:3804425
cn3	En1^tm2(cre)Wrst/? Gt(ROSA)26Sor^tm5(CAG-EGFP,-lacZ)Dym/? Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr Tg(Fev-flpe)1Dym/?	involves: 129S1/Sv * 129X1/SvJ	MGI:3804428
cn4	Egr2^tm2(cre)Pch/? Gt(ROSA)26Sor^tm5(CAG-EGFP,-lacZ)Dym/? Tg(Fev-flpe)1Dym/?	involves: 129S2/SvPas	MGI:3804426
cn5	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ Tmem163^Tg(ACTB-cre)2Mrt/0 Tg(Fev-flpe)1Dym/0	involves: 129S6/SvEvTac * FVB/N	MGI:4412290
cn6	Gt(ROSA)26Sor^tm5(CAG-EGFP,-lacZ)Dym/? Tg(Fev-flpe)1Dym/? Tg(Hoxa2-cre)1Dym/?	Not Specified	MGI:3804427

Genotype
MGI:4412291

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; En1^tm2(cre)Wrst/En1⁺; Tg(Fev-flpe)1Dym/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:154944 )

N • mice exhibit normal contextual fear conditioning and prepulse mediated inhibition of acoustic startle reflex

decreased anxiety-related response ( J:154944 )

nervous system

abnormal axon morphology ( J:154944 )

• 5HT+ axon varicosities are enlarged compared to in wild-type mice

Genotype
MGI:3804425

cn2

• Allelic Composition: En1^tm2(cre)Wrst/?; Gt(ROSA)26Sor^{tm5(CAG-EGFP,-lacZ)Dym}/?; Tg(Fev-flpe)1Dym/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

normal phenotype

no abnormal phenotype detected ( J:136096 )

• alleles are used in intersectional and subtractive cell lineage fate mapping to visualize serotonin (5-HT) producing neurons and their precursors in the E12.5 embryonic hindbrain or mature brainstem

• rhombomere-1 (r1) derived 5-HT precursor cells are labeled by beta-gal while 5-HT precursors arising caudal to r1 are labeled by eGFP

Genotype
MGI:3804428

cn3

• Allelic Composition: En1^tm2(cre)Wrst/?; Gt(ROSA)26Sor^{tm5(CAG-EGFP,-lacZ)Dym}/?; Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr; Tg(Fev-flpe)1Dym/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

nervous system

abnormal rhombomere morphology ( J:136096 )

• alleles are used in intersectional and subtractive cell lineage fate mapping to visualize serotonin (5-HT) producing neurons and their precursors in the P0.5 brainstem

• rhombomere-1 (r1) derived 5-HT precursor cells are not found in the B1-B3 areas of the brainstem

embryo

abnormal rhombomere morphology ( J:136096 )

• alleles are used in intersectional and subtractive cell lineage fate mapping to visualize serotonin (5-HT) producing neurons and their precursors in the P0.5 brainstem

• rhombomere-1 (r1) derived 5-HT precursor cells are not found in the B1-B3 areas of the brainstem

Genotype
MGI:3804426

cn4

• Allelic Composition: Egr2^tm2(cre)Pch/?; Gt(ROSA)26Sor^{tm5(CAG-EGFP,-lacZ)Dym}/?; Tg(Fev-flpe)1Dym/?
• Genetic Background: involves: 129S2/SvPas

normal phenotype

no abnormal phenotype detected ( J:136096 )

• alleles are used in intersectional and subtractive cell lineage fate mapping to visualize serotonin (5-HT) producing neurons and their precursors in the E12.5 embryonic hindbrain or mature brainstem

• rhombomere-3 (r3) rhombomere-5 (r5) derived 5-HT precursor cells are labeled by beta-gal while 5-HT precursors arising outside of r3 or r5 are labeled by eGFP

Genotype
MGI:4412290

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; Tmem163^{Tg(ACTB-cre)2Mrt}/0; Tg(Fev-flpe)1Dym/0
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

cardiovascular system

decreased heart rate ( J:231745 )

• heart rate is slower by 6-10% at P5 and P8

behavior/neurological

enhanced contextual conditioning behavior ( J:154944 )

increased exploration in new environment ( J:154944 )

decreased anxiety-related response ( J:154944 )

• mice are less averse to open, brightly lit spaces compared with wild-type mice

• female mice exhibit decreased anxiety-related behaviors in open field, zero-maze, and light-dark tests compared with wild-type mice

growth/size/body

decreased body size ( J:231745 )

• pups are smaller than controls by 15-21% at P5 and P8

nervous system

abnormal axon morphology ( J:154944 )

• 5HT+ axon varicosities are enlarged compared to in wild-type mice

increased prepulse inhibition ( J:154944 )

homeostasis/metabolism

abnormal oxygen consumption ( J:231745 )

• oxygen consumption is slightly but significantly lower at P5 and higher at P12

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:231745 )

• autoresuscitation is less effective compared to controls following repeated episodic exposure to anoxia and results in increased mortality at P5 and P8, with only 16% of pups surviving to the endpoint at P12; deaths occur during the anoxic challenge

respiratory system

increased tidal volume ( J:231745 )

• tidal volume is increased by 18-34% at P8 and P12

abnormal breathing pattern ( J:231745 )

• increase in baseline breathing variability at P5

• autoresuscitation is less effective compared to controls following repeated episodic exposure to anoxia and results in increased mortality at P5 and P8, with only 16% of pups surviving to the endpoint at P12

• pups exposed to anoxia that die at P5 take 4 times longer to initiate gasping and 4 times longer to recover heart rate and eupneic breathing to 90% of baseline

hypopnea ( J:231745 )

• pups have slower breathing than controls by 14% at P5 and P8

increased pulmonary ventilation ( J:231745 )

• 28% larger minute ventilation at P12

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
sudden infant death syndrome		DOID:9007	OMIM:272120	J:231745

Genotype
MGI:3804427

cn6

• Allelic Composition: Gt(ROSA)26Sor^{tm5(CAG-EGFP,-lacZ)Dym}/?; Tg(Fev-flpe)1Dym/?; Tg(Hoxa2-cre)1Dym/?
• Genetic Background: Not Specified

normal phenotype

no abnormal phenotype detected ( J:136096 )

• alleles are used in intersectional and subtractive cell lineage fate mapping to visualize serotonin (5-HT) producing neurons and their precursors in the E12.5 embryonic hindbrain or mature brainstem

• rhombomere-2 (r2) derived 5-HT precursor cells are labeled by beta-gal while 5-HT precursors arising outside of r2 are labeled by eGFP