About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Akt1tm1Pnt
targeted mutation 1, Philip N Tsichlis
MGI:3794787
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Tg(Lck-cre)1Cwi/0 		involves: 129P2/OlaHsd MGI:3803971
cn2
 		Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0 		involves: 129P2/OlaHsd MGI:3803972
cn3
 		Akt1tm1Pnt/Akt1tm1Pnt
Tg(Lck-cre)1Cwi/0 		Not Specified MGI:3803966
cn4
 		Akt1tm1Pnt/Akt1tm1Pnt
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0 		Not Specified MGI:3803970


Genotype
MGI:3803971
cn1
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Akt2tm1.1Mbb mutation (2 available); any Akt2 mutation (51 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased T cell apoptosis ( J:135903 )
• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 15-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 56.2% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 25% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are partially blocked at the DN3 to DN4 stage and the DN4 to the DP stage

hematopoietic system
increased T cell apoptosis ( J:135903 )
• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 15-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 56.2% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 25% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are partially blocked at the DN3 to DN4 stage and the DN4 to the DP stage

cellular
increased T cell apoptosis ( J:135903 )
• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)

endocrine/exocrine glands
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 15-fold




Genotype
MGI:3803972
cn2
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Akt2tm1.1Mbb mutation (2 available); any Akt2 mutation (51 available)
Akt3tm1Mbb mutation (0 available); any Akt3 mutation (42 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
increased T cell apoptosis ( J:135903 )
• double negative thymocytes of all stages have higher rates of apoptosis
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 20-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 85.4% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 1.8% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are almost completely blocked at the DN4 to DP transition

immune system
increased T cell apoptosis ( J:135903 )
• double negative thymocytes of all stages have higher rates of apoptosis
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 20-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 85.4% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 1.8% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are almost completely blocked at the DN4 to DP transition

cellular
increased T cell apoptosis ( J:135903 )
• double negative thymocytes of all stages have higher rates of apoptosis

endocrine/exocrine glands
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 20-fold




Genotype
MGI:3803966
cn3
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Tg(Lck-cre)1Cwi/0
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:135903 )
• no abnormalities in T cell development are observed




Genotype
MGI:3803970
cn4
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Akt3tm1Mbb mutation (0 available); any Akt3 mutation (42 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 2.5 fold
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 78.2% compared to 84.6% in wild-type mice

immune system
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 2.5 fold
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 78.2% compared to 84.6% in wild-type mice

endocrine/exocrine glands
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 2.5 fold




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory