Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smn1tm5(Smn1/SMN2)Mrph mutation
(4 available);
any
Smn1 mutation
(86 available)
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skeleton
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• 6 month old mutants exhibit lower bone mineral content
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• 6 month old mutants exhibit lower bone mineral density
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smn1tm5(Smn1/SMN2)Mrph mutation
(4 available);
any
Smn1 mutation
(86 available)
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skeleton
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• 6 month old mutants exhibit lower bone mineral content
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• 6 month old mutants exhibit lower bone mineral density
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smn1tm5(Smn1/SMN2)Mrph mutation
(4 available);
any
Smn1 mutation
(86 available)
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growth/size/body
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• by P15 and P20, males and females, respectively, are smaller than wild-type controls
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muscle
behavior/neurological
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• mutants perform better than controls on the accelerated rotarod test and marginally better on the constant speed rotarod test at 1 month of age
• however, forelimb grip strength is normal at 24 and 52 weeks of age
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• in plantar von Frey testing, 6-8, 20 and 24 week old mutants show significantly lower paw withdrawal force than controls, indicating hyperalgesia
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• 6-8 week old mutants (with no paw necrosis) exhibit an increase in sensitivity to moderately noxious heat with a significant decrease in hindpaw withdrawal latencies when placed on a 45C and 48C metal plate, however no differences in response to high noxious stimulus of 52C is seen
• 6-8 week old mutants exhibit robust sensitization in the evaporative cooling assay, responding more vigorously than controls, indicating increased sensitivity to temperature
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cardiovascular system
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• thermal imaging indicates that vascular destruction in the tail occurs before overt exterior necrosis
• lateral vasculature of the tail is primarily affected, with a loss of integrity in the vessel wall (most evident in the inner tunica intima of the small lateral vasculature) whereas the ventral artery remains intact until the very last stages of necrosis
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• 9 month old mutants exhibit lower average heart rate than controls
• however, mutants do not exhibit a longer PR or QRS interval, indicating that mutants do not exhibit bradyarrhythmia
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integument
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• mutants present with progressive necrosis of peripheral body parts beginning with the tail and moving toward the hindlimbs and then to the pinnae of the ears
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nervous system
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• at P90, about 10% of neuromuscular junctions (NMJs) in splenius, longissimus, and semispinalis muscles show abnormal morphology, with fragmented acetylcholine receptor clusters, abnormal thin nerve terminals, some junctions innervated by multiple nerve terminals, and nerve terminal sprouting, indicating that synaptic maintenance is mildly disrupted
• however, at P8 and P14, all NJMs are innervated and no nerve sprouting is seen, indicating that synapse formation at the NJM is not affected in mutants
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• reduction in synaptic transmission efficacy in muscle
• however, mutants exhibit normal number of motor axons in the lumbar 3 and lumbar 4 ventral roots at P350 and no significant loss of VGLUT1-positive synapses in L1-2 motoneurons
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• at P90, about 8% of junctions in splenius muscle are silent, where MEPPs could be recorded, but nerve stimulation did not elicit endplate potentials (EPPs)
• at P350, 15% of junctions in splenius muscles are silent in the functional junctions that remain
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• at P90 and P350, muscle exhibits a slight increase in the spontaneous miniature endplate potential (MEPP) amplitude
• upon nerve stimulation, the quantal content is decreased by 13-14% in both mutants at P90 and P350
• however, no difference from controls is seen in the MEPP frequency or the evoked endplate potential (EPP) amplitudes
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limbs/digits/tail
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• onset of tail necrosis is seen as early as P5 and progresses to complete loss of the tail by 45 days of age
• tail necrosis at 20-40 days of age is characterized by disorganization of muscle fiber bundles along with loss of intact vasculature in the absence of an inflammatory lesion as mice age
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cellular
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• mutants present with progressive necrosis of peripheral body parts beginning with the tail and moving toward the hindlimbs and then to the pinnae of the ears
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• onset of tail necrosis is seen as early as P5 and progresses to complete loss of the tail by 45 days of age
• tail necrosis at 20-40 days of age is characterized by disorganization of muscle fiber bundles along with loss of intact vasculature in the absence of an inflammatory lesion as mice age
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limbs/digits/tail
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• onset of tail necrosis (tail swelling and shortening) occurs at 6 weeks of age
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integument
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• mutants present with progressive necrosis of peripheral body parts beginning with the tail and moving toward the hindlimbs and then to the pinnae of the ears
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cellular
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• mutants present with progressive necrosis of peripheral body parts beginning with the tail and moving toward the hindlimbs and then to the pinnae of the ears
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• onset of tail necrosis (tail swelling and shortening) occurs at 6 weeks of age
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Analysis Tools