Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxna2tm1Hfu mutation
(1 available);
any
Plxna2 mutation
(127 available)
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nervous system
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• fibers invade the infrapyramidal region of Ca3c and the striatum pyramidalis of CA3ab rather than the suprapyramidal region
• in slice cocultures mossy fibers from homozygous mutant or wild-type dentate gyrus slices invade the infrapyramidal region or the stratum pyramidalis but not the suprapyramidal region of homozygous mutant CA3 slices
• however, paired pulse facilitation and long term potentiation are normal suggesting that mossy fiber synapses function normally
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxna2tm1Hfu mutation
(1 available);
any
Plxna2 mutation
(127 available)
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nervous system
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• about 1/3 of labeled granule cells remain in the external granule layer 36 h after a BrdU injection
• the number of migrating cells and maximum migration distance are reduced in external granule layer explants
• in migrating granule cells the average distance between the centrosome and nucleus is increased and more variable
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• mossy fiber terminate at the proximal parts of the infrapyramidal region and stratum and fail to innervate into the suprapyramidal region unlike in wild-type mice
• mice lack a suprapyramidal bundle unlike wild-type mice
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• about 40% of granule cells remain in the molecular layer in the adult cerebellum
• ectopic cells are still innervated by mossy fibers, neurite outgrowth from granule cells is normal and Purkinje cell differentiation is normal
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vision/eye
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N |
• mice exhibit normal retinal stratification
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cellular
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• about 1/3 of labeled granule cells remain in the external granule layer 36 h after a BrdU injection
• the number of migrating cells and maximum migration distance are reduced in external granule layer explants
• in migrating granule cells the average distance between the centrosome and nucleus is increased and more variable
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxna2tm1Hfu mutation
(1 available);
any
Plxna2 mutation
(127 available)
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immune system
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• increased lymphatic branching complexity
• however, lymphatic endothelial cell numbers are normal
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vision/eye
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• the starburst amacrine cell processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• On starburst amacrine cells are missing extensive portions of their dendritic fields and normal dendritic self-avoidance is compromised in the distal most third of the dendritic processes
• On starburst amacrine cells retain asymmetric dendritic arbors; however, Off cells develop the normal radially symmetrical dendritic pattern
• dendritic arborization defects of On SACs in the x-y plane are independent of the stratification defects in the z plane
• disruption of the dendritic plexus organization of On but not Off SACs
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• the starburst amacrine cell processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• dendrites of both On and Off starburst amacrine cells fail to stratify appropriately
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nervous system
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• On starburst amacrine cells are missing extensive portions of their dendritic fields and normal dendritic self-avoidance is compromised in the distal most third of the dendritic processes
• disruption of the dendritic plexus organization of On but not Off SACs
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• the starburst amacrine cell processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• On starburst amacrine cells are missing extensive portions of their dendritic fields and normal dendritic self-avoidance is compromised in the distal most third of the dendritic processes
• On starburst amacrine cells retain asymmetric dendritic arbors; however, Off cells develop the normal radially symmetrical dendritic pattern
• dendritic arborization defects of On SACs in the x-y plane are independent of the stratification defects in the z plane
• disruption of the dendritic plexus organization of On but not Off SACs
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• in culture both SEMA6A- and SEMA6A+ starburst amacrine cells from the retina extend neurites across strips coated with recombinant SEMA6A protein
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vision/eye
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• the starburst amacrine cell (SAC) processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• disruption of the dendritic plexus organization of On but not Off SACs
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• the starburst amacrine cell (SAC) processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
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nervous system
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• the starburst amacrine cell (SAC) processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• disruption of the dendritic plexus organization of On but not Off SACs
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• the starburst amacrine cell (SAC) processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• disruption of the dendritic plexus organization of On but not Off SACs
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nervous system
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N |
• unlike mice homozygous for Plxna2tm1Hfu alone mossy fiber projection is essentially normal
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• the suprapyramidal bundle is slightly thinner than in wild-type mice
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• the number of ectopic granule cells in the molecular layer is not significantly different from single homozygous mice
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vision/eye
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• the starburst amacrine cell processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• On starburst amacrine cells are missing extensive portions of their dendritic fields and normal dendritic self-avoidance is compromised in the distal most third of the dendritic processes
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• the starburst amacrine cell processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
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nervous system
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• On starburst amacrine cells are missing extensive portions of their dendritic fields and normal dendritic self-avoidance is compromised in the distal most third of the dendritic processes
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• the starburst amacrine cell processes that normally stratify in 2 discrete inner plexiform laminae (S2 and S4) fail to completely segregate from each other
• On starburst amacrine cells are missing extensive portions of their dendritic fields and normal dendritic self-avoidance is compromised in the distal most third of the dendritic processes
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxna2tm1Hfu mutation
(1 available);
any
Plxna2 mutation
(127 available)
Sema6btm1Hfu mutation
(0 available);
any
Sema6b mutation
(27 available)
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nervous system
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• mossy fibers initially grow into the proximal parts of the infrapyramidal region and stratum pyramidalis of CA3c and eventually invade proximal parts of the suprapyramidal region of CA3ab to form the thinner suprapyramidal bundle compared to in wild-type mice
• some mossy fibers directly invade the stratum radiatum and stratum oriens of the CA3c and sometimes grow into the stratum pyramidalis of CA1 unlike in wild-type mice
• the suprapyramidal bundle is thinner than in wild-type mice and Plxna2tm1Hfu Sema6aGt(KST069)Byg double homozygotes
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxna2tm1Hfu mutation
(1 available);
any
Plxna2 mutation
(127 available)
Sema6btm1Hfu mutation
(0 available);
any
Sema6b mutation
(27 available)
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nervous system
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• mossy fibers are restricted to proximal parts of the infrapyramidal region and stratum pyramidalis in most parts of CA3, except distal CA3ab, and form a very thin suprapyramidal bundle compared to in wild-type mice
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nervous system
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• mossy fibers are widely distributed in CA3 as in mice homozygous for Plxna4tm1Hfu alone
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Analysis Tools