Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f6tm1Lees mutation
(0 available);
any
E2f6 mutation
(12 available)
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skeleton
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• transformation of T13 to L1 is seen in 50% of homozygous mice
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• transformation of C5 to C6 is seen in 10% of homozygous mice
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• transformation of L6 to S1 is seen in 80% of homozygous mice
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hematopoietic system
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N |
• unlike mice homozygous for Bmi1tm1Brn no abnormalities in the numbers or distribution of hematopoietic cells is detected
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cellular
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N |
• unlike mice homozygous for Bmi1tm1Brn MEFs do not display any obvious defects in proliferation , senescence, or ability to re-enter the cell cycle
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Allelic
Composition |
E2f6tm1Lees/E2f6+
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Genetic
Background |
involves: 129S4/SvJae * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f6tm1Lees mutation
(0 available);
any
E2f6 mutation
(12 available)
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skeleton
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• transformation of T13 to L1 is seen in 10% of heterozygous mice
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• transformation of L6 to S1 is seen in 24% of heterozygous mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation
(2 available);
any
Bmi1 mutation
(33 available)
E2f6tm1Lees mutation
(0 available);
any
E2f6 mutation
(12 available)
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skeleton
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• small extra bone present above C1 in 62% of compound mutant mice
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• transformation of T7 to T8 and T13 to L1 are seen in 77% and 85% of compound mutant mice, respectively
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• transformation of C1 to C2, C5 to C6, and C7 to T1 are seen in 85%, 23%, and 31% of compound mutant mice, respectively
• partial transformation of C7 to T1 is seen in 77% of compound mutant mice
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• transformation of L6 to S1 is seen in 92% of compound mutant mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation
(2 available);
any
Bmi1 mutation
(33 available)
E2f6tm1Lees mutation
(0 available);
any
E2f6 mutation
(12 available)
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skeleton
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• transformation of T13 to L1 is seen in 77% of compound mutant mice
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• partial or complete transformation of C7 to T1 is seen in 27% and 4% of compound mutant mice, respectively
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• transformation of L6 to S1 is seen in 92% of compound mutant mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation
(2 available);
any
Bmi1 mutation
(33 available)
E2f6tm1Lees mutation
(0 available);
any
E2f6 mutation
(12 available)
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mortality/aging
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• lethality is seen between 3 and 20 weeks of age
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• only 38% of the expected number of mice are present at weaning
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• only 46% of the expected number of embryos are present at E18.5
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growth/size/body
skeleton
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• small extra bone present above C1 in 38% of double homozygous mice
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• transformation of T7 to T8 and T13 to L1 are seen in 62% and 100% of double homozygous mice, respectively
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• transformation of C1 to C2, C5 to C6, and C7 to T1 are seen in 92%, 23%, and 8% of double homozygous mice, respectively
• partial transformation of C7 to T1 is seen in 85% of double homozygous mice
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• transformation of L6 to S1 is seen in 100% of double homozygous mice
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hematopoietic system
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• shift in the distribution of myeloid to B lymphoid cells resulting in an increased percentage of myeloid cells
• within the B cell population, loss of immature B cells is greater than the loss of mature B cells
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• decreased number of hematopoietic cells in the thymus, bone marrow and spleen
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cellular
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• impaired ability of serum-starved MEFs to re-enter the cell cycle
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• defect in asynchronous proliferation in MEFs
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behavior/neurological
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• similar to that seen in mice homozygous for Bmi1tm1Brn alone at 2 months of age
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nervous system
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• all three layers are affected
• hypoplasia is similar to that in mice homozygous for Bmi1tm1Brn alone
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immune system
Allelic
Composition |
Bmi1tm1Brn/Bmi1+
E2f6tm1Lees/E2f6+
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Genetic
Background |
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation
(2 available);
any
Bmi1 mutation
(33 available)
E2f6tm1Lees mutation
(0 available);
any
E2f6 mutation
(12 available)
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skeleton
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• transformation of T7 to T8 and T13 to L1 are seen in 4% and 50% of double heterozygous mice, respectively
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• partial or complete transformation of C7 to T1 is seen in 42% and 8% of double heterozygous mice, respectively
• transformation of C5 to C6 is seen in 4% of double heterozygous mice
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• transformation of L6 to S1 is seen in 73% of double heterozygous mice
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Analysis Tools