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Allele Symbol Allele Name Allele ID |
Lcp2tm3Gak targeted mutation 3, Gary A Koretzky MGI:3778759 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice fail to delete Vbeta11+ and only partially delete Vbeta12+ thymocytes
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• mice fail to delete Vbeta11+ and only partially delete Vbeta12+ thymocytes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the majority of double-positive (DP) thymocytes fail to upregulate CD5 and CD2, which are indicators of pre-TCR signaling
• cultured DP thymocytes upon stimulation fail to lower expression of CD4 and CD8, and increase expression of Nur77
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• there is a partial defect in transitioning from DN3 cells to DN4 cells
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• there is a significant skewing towards CD4 single positive T cells in the ratio of CD4:CD8 T cells in the thymus
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• the frequency and absolute number of NKT in the thymus is drastically reduced
• the number of NKT cells is also reduced in the spleen and liver
• expression of NK1.1 on the remaining NKT cells in thymus, liver, and spleen is markedly diminished
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• DP and CD4 single positive thymocytes flux less calcium upon in vitro activation
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• the majority of double-positive (DP) thymocytes fail to upregulate CD5 and CD2, which are indicators of pre-TCR signaling
• cultured DP thymocytes upon stimulation fail to lower expression of CD4 and CD8, and increase expression of Nur77
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• there is a partial defect in transitioning from DN3 cells to DN4 cells
|
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• there is a significant skewing towards CD4 single positive T cells in the ratio of CD4:CD8 T cells in the thymus
|
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• the frequency and absolute number of NKT in the thymus is drastically reduced
• the number of NKT cells is also reduced in the spleen and liver
• expression of NK1.1 on the remaining NKT cells in thymus, liver, and spleen is markedly diminished
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• DP and CD4 single positive thymocytes flux less calcium upon in vitro activation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• thymocyte numbers and functionality are normal or near normal indicating the two mutant alleles complement each other
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• the reduced thymic cellularity of mice carrying just the transgenic TCR is rescued when crossed to the Lcp2tm3Gak homozygote background
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• the block in development at the double negative stage for mice carrying just the transgene is substantially alleviated when crossed to the Lcp2tm3Gak homozygote background
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• the number of double positive T cells in the thymus are increased compared to mice carrying just the transgenic TCR
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• the number of CD8 single positive T cells in the thymus are increased compared to mice carrying just the transgenic TCR
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• the block in development at the double negative stage for mice carrying just the transgene is substantially alleviated when crossed to the Lcp2tm3Gak homozygote background
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• the number of double positive T cells in the thymus are increased compared to mice carrying just the transgenic TCR
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• the number of CD8 single positive T cells in the thymus are increased compared to mice carrying just the transgenic TCR
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes
• upon conjugation, thymocytes have impaired TCR-induced actin polymerization
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• there is an approximate 75% loss of transgenic TCR expression by CD4 single positive cells in the thymus
• the percentage of CD4 single positive T cells in the thymus is reduced almost 20-fold compared mice carrying the transgenic TCR alone
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• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes
• upon conjugation, thymocytes have impaired TCR-induced actin polymerization
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• there is an approximate 75% loss of transgenic TCR expression by CD4 single positive cells in the thymus
• the percentage of CD4 single positive T cells in the thymus is reduced almost 20-fold compared mice carrying the transgenic TCR alone
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• 80% less B cells pulsed with antigen are conjugated by double positive thymocytes
• upon conjugation, thymocytes have impaired TCR-induced actin polymerization
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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