About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Col6a1-cre)1Gkl
transgene insertion 1, George Kollias
MGI:3775430
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA MGI:6195614
cn2
 		Tnftm2Gkl/Tnf+
Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA MGI:6195612
cn3
 		Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/? 		involves: 129S/SvEv * C57BL/6 * CBA MGI:3775437
cn4
 		Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: C57BL/6 * C57BL/6J * CBA MGI:6195560
cn5
 		Map3k8tm1.1Gkl/Map3k8tm1.1Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: C57BL/6 * CBA * FVB/N MGI:5476715


Genotype
MGI:6195614
cn1
Allelic
Composition		 Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1atm2Gkl mutation (1 available); any Tnfrsf1a mutation (47 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:264147 )
N
• aortic valves appear normal at 16 weeks of age, showing no signs of disease




Genotype
MGI:6195612
cn2
Allelic
Composition		 Tnftm2Gkl/Tnf+
Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1btm1.1Gkl mutation (0 available); any Tnfrsf1b mutation (41 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:264147 )
N
• mice show amelioration of the aortic valve stenosis seen in single Tnftm2Gkl heterozygotes, including a decrease of the leaflets surface area and reduction of fibrosis

skeleton
joint inflammation ( J:264147 )
• mice show reduced clinical features of arthritis compared to single Tnftm2Gkl heterozygotes, with a reduction of pannus formation and cartilage/bone destruction

cellular
abnormal cell physiology ( J:264147 )
• valvular interstitial cells fail to become activated
• however, cultured valvular interstitial cells appear similar to wild-type cells and exhibit normal proliferation
abnormal cell adhesion ( J:264147 )
• synovial fibroblast and valvular interstitial cell adhesion to a fibronectin substrate is reduced by almost 50% compared to the levels in single Tnftm2Gkl heterozygotes but is somewhat higher than in wild-type cells
abnormal fibroblast physiology ( J:264147 )
• synovial fibroblasts fail to become activated
• however, cultured synovial fibroblasts appear similar to wild-type cells and exhibit normal proliferation

homeostasis/metabolism
increased circulating tumor necrosis factor level ( J:264147 )
• circulating TNF levels are elevated
abnormal wound healing ( J:264147 )
• synovial fibroblasts and valvular interstitial cells show only 40-45% closure of a wound in culture compared to 70-80% closure in single Tnftm2Gkl heterozygotes and about 20% in wild-type cells

immune system
increased circulating tumor necrosis factor level ( J:264147 )
• circulating TNF levels are elevated
increased tumor necrosis factor secretion ( J:264147 )
• levels of secreted TNF-alpha in supernatants of cultured synovial fibroblasts and valvular interstitial cells are elevated
joint inflammation ( J:264147 )
• mice show reduced clinical features of arthritis compared to single Tnftm2Gkl heterozygotes, with a reduction of pannus formation and cartilage/bone destruction




Genotype
MGI:3775437
cn3
Allelic
Composition		 Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/?
Genetic
Background		 involves: 129S/SvEv * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1atm2Gkl mutation (1 available); any Tnfrsf1a mutation (47 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
small intestinal inflammation ( J:131930 )
• the ileum contains inflammatory pathology reminiscent of Crohn's disease
• pathology includes blunting of intestinal villi, and infiltration of inflammation cells into the mucosal and submucosal layers
• 100% of mice have disease by 8 weeks of age

immune system
small intestinal inflammation ( J:131930 )
• the ileum contains inflammatory pathology reminiscent of Crohn's disease
• pathology includes blunting of intestinal villi, and infiltration of inflammation cells into the mucosal and submucosal layers
• 100% of mice have disease by 8 weeks of age
arthritis ( J:131930 )
• 100% of mice have arthritis by 8 weeks of age
• joints are infiltrated by mononuclear cells
• mice also develop spondyloarthritis in their sacroiliac joints

skeleton
arthritis ( J:131930 )
• 100% of mice have arthritis by 8 weeks of age
• joints are infiltrated by mononuclear cells
• mice also develop spondyloarthritis in their sacroiliac joints




Genotype
MGI:6195560
cn4
Allelic
Composition		 Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1btm1.1Gkl mutation (0 available); any Tnfrsf1b mutation (41 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:264147 )
• mice are born at the expected ratios, are viable, and fertile and do not display any spontaneous phenotypes




Genotype
MGI:5476715
cn5
Allelic
Composition		 Map3k8tm1.1Gkl/Map3k8tm1.1Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: C57BL/6 * CBA * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map3k8tm1.1Gkl mutation (1 available); any Map3k8 mutation (41 available)
Tg(Col6a1-cre)1Gkl mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
decreased enterocyte apoptosis ( J:193546 )
• decreased in mice treated with AOM/DSS
abnormal enterocyte proliferation ( J:193546 )
• increased in mice treated with AOM/DSS
abnormal colon morphology ( J:193546 )
• high-grade dysplasia in mice treated with AOM/DSS

neoplasm
increased incidence of tumors by chemical induction ( J:193546 )
• higher tumor burdens and tumor size in mice treated with AOM/DSS
increased tumor growth/size ( J:193546 )
• higher tumor burdens and tumor size in mice treated with AOM/DSS

cellular
decreased enterocyte apoptosis ( J:193546 )
• decreased in mice treated with AOM/DSS
abnormal enterocyte proliferation ( J:193546 )
• increased in mice treated with AOM/DSS

homeostasis/metabolism
homeostasis/metabolism phenotype ( J:193546 )
N
• mice treated with AOM/DSS exhibit normal body weight loss, decreased colon length, inflammation and tissue damage
increased incidence of tumors by chemical induction ( J:193546 )
• higher tumor burdens and tumor size in mice treated with AOM/DSS




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory