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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tcf12tm3Zhu
targeted mutation 3, Yuan Zhuang
MGI:3769702
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Tcf12tm3Zhu/Tcf12+
Tg(EIIa-cre)C5379Lmgd/0
Twist1tm1Bhr/Twist1+ 		involves: 129 * 129S7/SvEvBrd * C57BL/6 * FVB/N MGI:8247991
cn2
 		Tcf12tm3Zhu/Tcf12+
Tg(EIIa-cre)C5379Lmgd/0 		involves: 129 * C57BL/6 * FVB/N MGI:8247990
cn3
 		Tcf12tm3Zhu/Tcf12tm3Zhu
Tcf3tm4Zhu/Tcf3tm4Zhu
Tg(Cd4-cre)1Cwi/0 		involves: 129S4/SvJae * C57BL/6 * DBA/2 MGI:3769738
cn4
 		Tcf12tm3Zhu/Tcf12tm3Zhu
Tcf3tm4Zhu/Tcf3tm4Zhu
Tg(Lck-cre)#Zhu/0 		involves: 129S4/SvJae * C57BL/6 * SJL/J MGI:3769725
cn5
 		Tcf12tm3Zhu/Tcf12tm3Zhu
Tg(Lck-cre)#Zhu/0 		involves: C57BL/6 * SJL/J MGI:3769726


Genotype
MGI:8247991
cn1
Allelic
Composition		 Tcf12tm3Zhu/Tcf12+
Tg(EIIa-cre)C5379Lmgd/0
Twist1tm1Bhr/Twist1+
Genetic
Background		 involves: 129 * 129S7/SvEvBrd * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm3Zhu mutation (1 available); any Tcf12 mutation (75 available)
Tg(EIIa-cre)C5379Lmgd mutation (5 available)
Twist1tm1Bhr mutation (4 available); any Twist1 mutation (19 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
premature coronal suture closure ( J:198060 )
• mice show severe coronal synostosis

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
craniosynostosis DOID:2340 OMIM:182212
OMIM:600593
 J:198060




Genotype
MGI:8247990
cn2
Allelic
Composition		 Tcf12tm3Zhu/Tcf12+
Tg(EIIa-cre)C5379Lmgd/0
Genetic
Background		 involves: 129 * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm3Zhu mutation (1 available); any Tcf12 mutation (75 available)
Tg(EIIa-cre)C5379Lmgd mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
skeleton phenotype ( J:198060 )
N
• mice exhibit normal coronal sutures




Genotype
MGI:3769738
cn3
Allelic
Composition		 Tcf12tm3Zhu/Tcf12tm3Zhu
Tcf3tm4Zhu/Tcf3tm4Zhu
Tg(Cd4-cre)1Cwi/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm3Zhu mutation (1 available); any Tcf12 mutation (75 available)
Tcf3tm4Zhu mutation (2 available); any Tcf3 mutation (48 available)
Tg(Cd4-cre)1Cwi mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell differentiation ( J:129171 )
• T cells prematurely acquire single positive phenotype compared to in wild-type mice
abnormal CD8-positive, alpha beta T cell morphology ( J:129171 )
• mice present with CD+TCR- T cell population not found in controls

hematopoietic system
abnormal T cell differentiation ( J:129171 )
• T cells prematurely acquire single positive phenotype compared to in wild-type mice
abnormal CD8-positive, alpha beta T cell morphology ( J:129171 )
• mice present with CD+TCR- T cell population not found in controls




Genotype
MGI:3769725
cn4
Allelic
Composition		 Tcf12tm3Zhu/Tcf12tm3Zhu
Tcf3tm4Zhu/Tcf3tm4Zhu
Tg(Lck-cre)#Zhu/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6 * SJL/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm3Zhu mutation (1 available); any Tcf12 mutation (75 available)
Tcf3tm4Zhu mutation (2 available); any Tcf3 mutation (48 available)
Tg(Lck-cre)#Zhu mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased T cell proliferation ( J:129570 )
• double negative 3 thymocytes exhibit a 1.5-fold increase in proliferation (20.1% proliferation compared to 12.7% proliferation in wild-type mice)
• double negative T cell proliferation in response to IL-7 is increased 3- to 4-fold compared to in wild-type mice
• double negative 3 thymocytes exhibit hyperproliferation in response to IL-7 compared to wild-type cells
thymus hypoplasia ( J:129570 )
• thymus cellularity is decreased 20-fold (6.7+/-3.1x106 cells compared to 134.4+/-33.4x106 cells in wild-type thymus)
increased double-negative T cell number ( J:129570 )
• T cell differentiation is blocked at the double negative stage
• double negative cells make up 95% of total thymus cells compared to only 3% in wild-type mice
• the number of double negative T cells is increased 50% compared to in wild-type mice despite hypocellularity
decreased T cell number ( J:129570 )
• gamma delta T cells are reduced 2- to 4-fold compared to in wild-type mice
decreased double-positive T cell number ( J:129570 )
• mice exhibit less than 1% of the double positive T cells found in wild-type mice

hematopoietic system
increased T cell proliferation ( J:129570 )
• double negative 3 thymocytes exhibit a 1.5-fold increase in proliferation (20.1% proliferation compared to 12.7% proliferation in wild-type mice)
• double negative T cell proliferation in response to IL-7 is increased 3- to 4-fold compared to in wild-type mice
• double negative 3 thymocytes exhibit hyperproliferation in response to IL-7 compared to wild-type cells
thymus hypoplasia ( J:129570 )
• thymus cellularity is decreased 20-fold (6.7+/-3.1x106 cells compared to 134.4+/-33.4x106 cells in wild-type thymus)
increased double-negative T cell number ( J:129570 )
• T cell differentiation is blocked at the double negative stage
• double negative cells make up 95% of total thymus cells compared to only 3% in wild-type mice
• the number of double negative T cells is increased 50% compared to in wild-type mice despite hypocellularity
decreased T cell number ( J:129570 )
• gamma delta T cells are reduced 2- to 4-fold compared to in wild-type mice
decreased double-positive T cell number ( J:129570 )
• mice exhibit less than 1% of the double positive T cells found in wild-type mice

endocrine/exocrine glands
thymus hypoplasia ( J:129570 )
• thymus cellularity is decreased 20-fold (6.7+/-3.1x106 cells compared to 134.4+/-33.4x106 cells in wild-type thymus)

cellular
increased T cell proliferation ( J:129570 )
• double negative 3 thymocytes exhibit a 1.5-fold increase in proliferation (20.1% proliferation compared to 12.7% proliferation in wild-type mice)
• double negative T cell proliferation in response to IL-7 is increased 3- to 4-fold compared to in wild-type mice
• double negative 3 thymocytes exhibit hyperproliferation in response to IL-7 compared to wild-type cells




Genotype
MGI:3769726
cn5
Allelic
Composition		 Tcf12tm3Zhu/Tcf12tm3Zhu
Tg(Lck-cre)#Zhu/0
Genetic
Background		 involves: C57BL/6 * SJL/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm3Zhu mutation (1 available); any Tcf12 mutation (75 available)
Tg(Lck-cre)#Zhu mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased thymocyte number ( J:129570 )
• 39.3+/-15.4x106 compared to 133+/-32.6x106 in wild-type mice
increased CD8-positive, alpha-beta T cell number ( J:129570 )
• mice exhibit an accumulation of CD8+ single positive T cells

hematopoietic system
decreased thymocyte number ( J:129570 )
• 39.3+/-15.4x106 compared to 133+/-32.6x106 in wild-type mice
increased CD8-positive, alpha-beta T cell number ( J:129570 )
• mice exhibit an accumulation of CD8+ single positive T cells

endocrine/exocrine glands
decreased thymocyte number ( J:129570 )
• 39.3+/-15.4x106 compared to 133+/-32.6x106 in wild-type mice




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Send questions and comments to User Support. 		last database update
10/07/2025
MGI 6.24 		The Jackson Laboratory