Phenotypes associated with this allele

• Allele Symbol: Del(11Hoxb3-Hoxb4)1Karl
• Allele Name: deletion, Chr 11, Stefan Karlsson 1
• Allele ID: MGI:3762002
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3762003
cx2	Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl Hoxa9^tm1Mrc/Hoxa9^tm1Mrc	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6	MGI:3762008

Genotype
MGI:3762003

hm1

• Allelic Composition: Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal definitive hematopoiesis ( J:83563 )

abnormal common myeloid progenitor cell morphology ( J:83563 )

• the absolute number of spleen colony forming units at day 12 was slightly reduced

• when cultured in a multicytokine mixture, Lin-Sca-1+c-Kit+ (LSK) bone marrow cells display a proliferative disadvantage relative to wild-type cells

decreased bone marrow cell number ( J:83563 )

• bone marrow cellularity is reduced by about 25% (34+/-5x10⁶ cells in two femurs compared to 45+/-6x10⁶ cells in two femurs in wild-type mice)

• however, cell proliferation is normal

decreased erythrocyte cell number ( J:83563 )

• 7.4+/-0.7x10¹² cells/L compared to 8.1+/-0.8x10¹² cells/L in wild-type mice

decreased hemoglobin content ( J:83563 )

• 139+/-10 g/L compared to 151+/-10 g/L in wild-type mice

abnormal hematopoietic stem cell morphology ( J:83563 )

• hematopoietic stem cells from mice treated with cyclophosphamide exhibit decreased proliferative capacity compared to cells from similarly treated wild-type mice

abnormal spleen morphology ( J:83563 )

decreased spleen weight ( J:83563 )

• spleen weight is reduced by 30% (70+/-7 mg compared to 103+/-14 mg in wild-type mice)

spleen hypoplasia ( J:83563 )

• spleen cellularity is reduced by 30% (127+/-24x10⁶ cells compared to 187+/-30x10⁶ cells in wild-type mice)

• however, cell proliferation is normal

liver/biliary system

abnormal liver morphology ( J:83563 )

• at E14.5, the number of Lin-ScaI+ AA4.1 cells is reduced (29+/-12x10³ cells compared to 49+/-22x10³ cells in wild-type mice)

growth/size/body

decreased body weight ( J:83563 )

• mice weight 8% less than wild-type mice at time of weaning

• however, this difference disappears by 12 weeks of age

skeleton

skeleton phenotype ( J:83563 )

N • unlike mice carrying Hoxb4^tm1.1Karl targeted mutation, formation of the thorax and other skeletal structures is normal

immune system

abnormal spleen morphology ( J:83563 )

decreased spleen weight ( J:83563 )

• spleen weight is reduced by 30% (70+/-7 mg compared to 103+/-14 mg in wild-type mice)

spleen hypoplasia ( J:83563 )

• spleen cellularity is reduced by 30% (127+/-24x10⁶ cells compared to 187+/-30x10⁶ cells in wild-type mice)

• however, cell proliferation is normal

Genotype
MGI:3762008

cx2

• Allelic Composition: Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl; Hoxa9^tm1Mrc/Hoxa9^tm1Mrc
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6

hematopoietic system

abnormal common myeloid progenitor cell morphology ( J:126371 )

• while the number of colonies formed from cultured bone marrow cells is normal, the size of colonies formed is smaller than in wild-type mice

decreased bone marrow cell number ( J:126371 )

• 4.3+/-0.3x10⁶ cells in 2 femurs compared to 6.0+/-0.4x10⁶ cells in 2 wild-type femurs

• however, red and white blood cell and hematocrit levels are normal

increased hematopoietic stem cell number ( J:126371 )

• the number of hematopoietic stem cells (Lin-Sca-1+c-Kit+CD150+ and Lin-Sca-1+c-Kit+CD34-) in bone marrow is increased 3-fold compared to in wild-type mice

decreased spleen weight ( J:126371 )

• 46.3+/-2.3 mg compared to 96.3+/-4.4 g in wild-type mice and 81.4+/-3.2 mgin Hoxa9^tm1Mrc homozygotes

spleen hypoplasia ( J:126371 )

• 8.9+/-0.3x10⁷ cells compared to 22.0+/-2.1x10⁷ cells in wild-type mice and 15.5+/-0.8x10⁷ cells in Hoxa9^tm1Mrc homozygotes

growth/size/body

decreased body weight ( J:126371 )

• 22.7+/-0.9 g compared to 31.5+/-1.2 g for wild-type mice and 27.6+/-1.0 g for Hoxa9^tm1Mrc homozygotes

immune system

decreased spleen weight ( J:126371 )

• 46.3+/-2.3 mg compared to 96.3+/-4.4 g in wild-type mice and 81.4+/-3.2 mgin Hoxa9^tm1Mrc homozygotes

spleen hypoplasia ( J:126371 )

• 8.9+/-0.3x10⁷ cells compared to 22.0+/-2.1x10⁷ cells in wild-type mice and 15.5+/-0.8x10⁷ cells in Hoxa9^tm1Mrc homozygotes