Phenotypes associated with this allele

• Allele Symbol: Pgm3^mld1
• Allele Name: multilineage defect 1
• Allele ID: MGI:3758645
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pgm3^mld1/Pgm3^mld1	C57BL/6-Pgm3^mld1	MGI:3758651
ht2	Pgm3^Gt(W037B08)Wrst/Pgm3^mld1	involves: 129S1/Sv * C57BL/6	MGI:3758652

Genotype
MGI:3758651

hm1

• Allelic Composition: Pgm3^mld1/Pgm3^mld1
• Genetic Background: C57BL/6-Pgm3^mld1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal spermatogenesis ( J:125094 )

♂ • while immature sperm is detected all mature sperm are pyknotic

male infertility ( J:125094 )

♂ • irregardless of the genotype of the females, male mice never sire offspring

hematopoietic system

increased spleen weight ( J:125094 )

• spleen weight is increased (166+/-34 mg compared to 87+/-13 mg in wild-type mice)

abnormal erythropoiesis ( J:125094 )

• mild anemia is accompanied by a compensatory extramedullary hematopoiesis, splenomegaly, expansion of red pulp and increase in the number of erythroid colony-forming units and erythropoietic burst formations

increased pro-B cell number ( J:125094 )

• the number of B220+ CD43+ pro-B cells are increased 2- to 3-fold relative to in wild-type mice

abnormal thrombopoiesis ( J:125094 )

• platelets are produced more slowly than in wild-type mice but are cleared at the same rate

extramedullary hematopoiesis ( J:125094 )

• extramedullary hematopoiesis occurs in the spleen

anemia ( J:125094 )

decreased bone marrow cell number ( J:125094 )

• bone marrow cell number is reduced to 70% of that observed in wild-type mice

abnormal megakaryocyte progenitor cell morphology ( J:125094 )

• bone marrow megakaryocyte progenitor cell number is reduced but bone marrow megakaryocyte cell number is normal to in wild-type mice

• spleen megakaryocyte progenitor cell and megakaryocyte cell numbers are increased relative to in wild-type mice

decreased erythrocyte cell number ( J:125094 )

• 8.0+/-0.62 x10⁹/ml compared to 10.4+/-0.5 x10⁹/ml in wild-type mice

decreased hematocrit ( J:125094 )

• 45.8+/-3.0% compared to 53.2+/-2.4% in wild-type mice

thrombocytopenia ( J:125094 )

• 620+/-128x10⁶/ml compared to 1159+/-176x10⁶/ml in wild-type mice

decreased leukocyte cell number ( J:125094 )

• 3.7+/-1.3x10⁶/ml compared to 9.2+/-1.9x10⁶/ml in wild-type mice

decreased B cell number ( J:125094 )

• the frequency and number of B cells at all later stages of maturation is reduced and the B cell number in the peritoneal cavity and peripheral lymphoid organs is reduced

absent follicular B cells ( J:125094 )

• lymphoid follicles are absent

increased spleen red pulp amount ( J:125094 )

digestive/alimentary system

abnormal pancreatic acinus morphology ( J:125094 )

• acini are reduced in size and dispersed

abnormal salivary gland morphology ( J:125094 )

• salivary gland architecture is disrupted

• cells are reduced in size and dispersed

abnormal feces composition ( J:125094 )

• protein fecal content is increased relative to in wild-type mice

immune system

increased spleen weight ( J:125094 )

• spleen weight is increased (166+/-34 mg compared to 87+/-13 mg in wild-type mice)

increased pro-B cell number ( J:125094 )

• the number of B220+ CD43+ pro-B cells are increased 2- to 3-fold relative to in wild-type mice

abnormal thrombopoiesis ( J:125094 )

• platelets are produced more slowly than in wild-type mice but are cleared at the same rate

decreased leukocyte cell number ( J:125094 )

• 3.7+/-1.3x10⁶/ml compared to 9.2+/-1.9x10⁶/ml in wild-type mice

decreased B cell number ( J:125094 )

• the frequency and number of B cells at all later stages of maturation is reduced and the B cell number in the peritoneal cavity and peripheral lymphoid organs is reduced

absent follicular B cells ( J:125094 )

• lymphoid follicles are absent

increased spleen red pulp amount ( J:125094 )

endocrine/exocrine glands

abnormal salivary gland morphology ( J:125094 )

• salivary gland architecture is disrupted

• cells are reduced in size and dispersed

abnormal pancreas morphology ( J:125094 )

• pancreatic architecture is disrupted

abnormal pancreatic acinus morphology ( J:125094 )

• acini are reduced in size and dispersed

growth/size/body

increased spleen weight ( J:125094 )

• spleen weight is increased (166+/-34 mg compared to 87+/-13 mg in wild-type mice)

Genotype
MGI:3758652

ht2

• Allelic Composition: Pgm3^{Gt(W037B08)Wrst}/Pgm3^mld1
• Genetic Background: involves: 129S1/Sv * C57BL/6

mortality/aging

postnatal lethality ( J:125094 )

• fewer than expect mice survive to weaning

reproductive system

abnormal spermatogenesis ( J:125094 )

♂ • while immature spermatpgonia is detected all mature spermtocytes are pyknotic

male infertility ( J:125094 )

♂ • irregardless of the genotype of the females, male mice never sire offspring

hematopoietic system

increased pro-B cell number ( J:125094 )

• the number of B220+ CD43+ pro-B cells are increased 2-fold relative to in wild-type mice

abnormal T cell differentiation ( J:125094 )

• mice exhibit an increase in the ratio of CD4+ to CD8+ T cells in the lymph nodes relative to wild-type mice

anemia ( J:125094 )

decreased bone marrow cell number ( J:125094 )

• bone marrow cell number is reduced to 50% of that observed in wild-type mice

decreased erythrocyte cell number ( J:125094 )

• 6.5+/-2.0 x10⁹/ml compared to 8.0+/-0.62x10⁹/ml in Pgm3^mld1 homozygotes and 10.4+/-0.5 x10⁹/ml in wild-type mice

decreased hematocrit ( J:125094 )

• 37.9+/-10.9% compared to 45.8+/-3.0% in Pgm3^mld1 homozygotes and 53.2+/-2.4% in wild-type mice

thrombocytopenia ( J:125094 )

• 858+/-312x10⁶/ml compared to 620+/-128x10⁶/ml in Pgm3^mld1 homozygotes and 1159+/-176x10⁶/ml in wild-type mice

decreased leukocyte cell number ( J:125094 )

• 4.8+/-2.1x10⁶/ml compared to 3.7+/-1.3x10⁶/ml in Pgm3^mld1 homozygotes and 9.2+/-1.9x10⁶/ml in wild-type mice

decreased B cell number ( J:125094 )

• the frequency and number of B cells at all later stages of maturation is reduced and the B cell number in the peritoneal cavity and peripheral lymphoid organs is reduced

absent follicular B cells ( J:125094 )

• lymphoid follicles are absent

decreased T cell number ( J:125094 )

• the number of T cells in the spleen is moderately to severely reduced compared to in wild-type mice

increased spleen red pulp amount ( J:125094 )

digestive/alimentary system

pancreatic acinar hypoplasia ( J:125094 )

• acini are reduced in size and dispersed

abnormal salivary gland morphology ( J:125094 )

• salivary gland architecture is disrupted

• cells are reduced in size and dispersed

abnormal feces composition ( J:125094 )

• protein fecal content is increased relative to in wild-type mice

renal/urinary system

glomerulonephritis ( J:125094 )

• mice exhibit glomerulonephritis with amorphous material filling the glomeruli

immune system

increased pro-B cell number ( J:125094 )

• the number of B220+ CD43+ pro-B cells are increased 2-fold relative to in wild-type mice

abnormal T cell differentiation ( J:125094 )

• mice exhibit an increase in the ratio of CD4+ to CD8+ T cells in the lymph nodes relative to wild-type mice

decreased leukocyte cell number ( J:125094 )

• 4.8+/-2.1x10⁶/ml compared to 3.7+/-1.3x10⁶/ml in Pgm3^mld1 homozygotes and 9.2+/-1.9x10⁶/ml in wild-type mice

decreased B cell number ( J:125094 )

• the frequency and number of B cells at all later stages of maturation is reduced and the B cell number in the peritoneal cavity and peripheral lymphoid organs is reduced

absent follicular B cells ( J:125094 )

• lymphoid follicles are absent

decreased T cell number ( J:125094 )

• the number of T cells in the spleen is moderately to severely reduced compared to in wild-type mice

increased spleen red pulp amount ( J:125094 )

glomerulonephritis ( J:125094 )

• mice exhibit glomerulonephritis with amorphous material filling the glomeruli

endocrine/exocrine glands

abnormal salivary gland morphology ( J:125094 )

• salivary gland architecture is disrupted

• cells are reduced in size and dispersed

abnormal pancreas morphology ( J:125094 )

• pancreatic architecture is disrupted

pancreatic acinar hypoplasia ( J:125094 )

• acini are reduced in size and dispersed