Phenotypes associated with this allele

• Allele Symbol: Pdcd1lg2^tm3Shr
• Allele Name: targeted mutation 3, Arlene H Sharpe
• Allele ID: MGI:3720328
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pdcd1lg2^tm3Shr/Pdcd1lg2^tm3Shr	involves: C57BL/6 * NOD/MrkTac	MGI:3721515
cx2	Cd274^tm1Shr/Cd274^tm1Shr Pdcd1lg2^tm3Shr/Pdcd1lg2^tm3Shr	C.B6-Cd274^tm1Shr Pdcd1lg2^tm3Shr	MGI:3721513

Genotype
MGI:3721515

hm1

• Allelic Composition: Pdcd1lg2^tm3Shr/Pdcd1lg2^tm3Shr
• Genetic Background: involves: C57BL/6 * NOD/MrkTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

insulitis ( J:123785 )

• 48% of mice aged from 27 to 50 days exhibit insulitis compared to none of the wild-type mice

increased CD4-positive, alpha-beta T cell number ( J:123785 )

• CD4+ T cells in the pancreatic lymph node is increased fourfold and twofold in diabetic mice

increased CD8-positive, alpha-beta T cell number ( J:123785 )

• CD8+ T cells in the pancreatic lymph node is increased fourfold and twofold in diabetic mice

abnormal CD8-positive, alpha-beta T cell physiology ( J:123785 )

• the percentage of CD8+ cells producing interferon-gamma, tumor necrotic factor or both was increased 2- to 3-fold compared to wild-type NOD mice

lymph node hyperplasia ( J:123785 )

• pancreatic lymph node cellularity is increased

• CD4+ and CD8+ T cells in the pancreatic lymph node is increased fourfold

increased susceptibility to autoimmune diabetes ( J:123785 )

• onset of automimmune diabetes mellitus is accelerated, sex-specific disease progression is lost, and penetrance is complete

• all mice develop diabetes by week 40 whereas only one tenth of wild-type mice have diabetes at that time point

homeostasis/metabolism

hyperglycemia ( J:123785 )

• onset of hyperglycemia occurs at 7.3 weeks when wild-type mice are euglycemic

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:123785 )

• CD4+ T cells in the pancreatic lymph node is increased fourfold and twofold in diabetic mice

increased CD8-positive, alpha-beta T cell number ( J:123785 )

• CD8+ T cells in the pancreatic lymph node is increased fourfold and twofold in diabetic mice

abnormal CD8-positive, alpha-beta T cell physiology ( J:123785 )

• the percentage of CD8+ cells producing interferon-gamma, tumor necrotic factor or both was increased 2- to 3-fold compared to wild-type NOD mice

endocrine/exocrine glands

insulitis ( J:123785 )

• 48% of mice aged from 27 to 50 days exhibit insulitis compared to none of the wild-type mice

Genotype
MGI:3721513

cx2

• Allelic Composition: Cd274^tm1Shr/Cd274^tm1Shr; Pdcd1lg2^tm3Shr/Pdcd1lg2^tm3Shr
• Genetic Background: C.B6-Cd274^tm1Shr Pdcd1lg2^tm3Shr

immune system

abnormal dendritic cell physiology ( J:123785 )

• bone marrow dendritic cells increases the production of IL-2 and interferon-gamma from DO11 transgenic T cells in vitro by 300% to 400% when compared to wild-type cells

increased interferon-gamma secretion ( J:123785 )

• in an in vitro assay with dendritic cell or an adoptive transfer experiment into mice, DO11 transgenic T cells are stimulated to over-produce interferon-gamma

increased interleukin-2 secretion ( J:123785 )

• in an in vitro assay with dendritic cell or an adoptive transfer experiment into mice, DO11 transgenic T cells are stimulated to over-produce IL-2

decreased interleukin-4 secretion ( J:123785 )

• bone marrow dendritic cells fail to stimulate the production of IL-4 from DO11 T cells in vitro or in adoptive transfer experiments

• in an adoptive transfer experiment into mice, IL-4+ DO11 transgenic cells are reduced by 86%