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Allele Symbol Allele Name Allele ID |
Artm1Jdz targeted mutation 1, Jeffrey D Zajac MGI:3717246 |
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| Summary |
9 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• testis weights at 3 weeks are significantly lower (3.5 mg) than control males (24.9-27 mg)
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• testis weights at 3 weeks are significantly lower (3.5 mg) than control males (24.9-27 mg)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• total body weights of males are equivalent to wild-type males
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• tubules with smaller diameters are observed at 9 weeks
• at 9 weeks total Sertoli cell numbers are same as in controls
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• at 9 weeks, interstitial Leydig cells display hypertrophy and accumulation of lipid droplets
• at 9 weeks adult Leydig cells are present in testes
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• Leydig cells display hypertrophy at 9 weeks
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• at 5 or 10 days, testis weights are normal, but testis weights at 3 weeks are significantly lower (17.9 mg) than control males (24.9-27 mg)
• at 9 weeks of age, testis weight are significantly lower than in controls but are 51% higher than in Tg(AMH-cre)1Flor/ Artm1Jdz double mutants
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• testes display more post meiotic development than hemizygous transgenic cre/Ar mutant mice, with elongated spermatids and lumen present in some tubules
• clusters of cells resembling abnormal round spermatids and spermatozoa are present in ducts of rete testes, indicating premature detachment of postmeiotic cells from germinal epithelium
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• tubules with smaller diameters are observed at 9 weeks
• at 9 weeks total Sertoli cell numbers are same as in controls
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• at 9 weeks, interstitial Leydig cells display hypertrophy and accumulation of lipid droplets
• at 9 weeks adult Leydig cells are present in testes
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• Leydig cells display hypertrophy at 9 weeks
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• at 5 or 10 days, testis weights are normal, but testis weights at 3 weeks are significantly lower (17.9 mg) than control males (24.9-27 mg)
• at 9 weeks of age, testis weight are significantly lower than in controls but are 51% higher than in Tg(AMH-cre)1Flor/ Artm1Jdz double mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
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• tubules with smaller diameters are observed at 9 weeks
• total Sertoli cell numbers are same as in controls at 9 weeks
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• testis weights at 3 weeks are significantly lower than control males (24.9-27 mg); at 9 weeks of age, weights are 34% lower than in males hemizygous for Tg(Abpa-cre)1Cmal Artm1Jdz mutants
• testis weights are similar to those of hemizygous or homozygous Tg(AMH-cre)1Flor/Artm1Jdz mice
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• tubules with smaller diameters are observed at 9 weeks
• total Sertoli cell numbers are same as in controls at 9 weeks
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• testis weights at 3 weeks are significantly lower than control males (24.9-27 mg); at 9 weeks of age, weights are 34% lower than in males hemizygous for Tg(Abpa-cre)1Cmal Artm1Jdz mutants
• testis weights are similar to those of hemizygous or homozygous Tg(AMH-cre)1Flor/Artm1Jdz mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 9 weeks of age, testis weights are similar to those of homozygous Tg(AMH-cre)1Flor or homozygous Tg(Abpa-cre)1Cmal/ Artm1Jdz double mutants
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• at 9 weeks of age, testis weights are similar to those of homozygous Tg(AMH-cre)1Flor or homozygous Tg(Abpa-cre)1Cmal/ Artm1Jdz double mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• despite a normal macroscopic appearance with normal epithelial branching and normal numbers of ductal tips, all prostate lobes are small by weight
• stroma is reduced and eosinophilic secretion in prostatic lumen is limited
• abnormal clustering of epithelial cells in the anterior lobe and to a lesser extent in the dorsolateral and ventral prostate are present
• increased proliferation of epithelial cells in the anterior prostate
• increased density of basal epithelial cells surrounding the abnormal epithelial cell clusters in the anterior prostate
• absolute volume of each cellular compartment (stroma, lumen, and epithelium) is significantly reduced in the dorsolateral and anterior prostate lobes
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• increased proliferation and abnormal lesions of epithelial cells predominantly in the anterior prostate lobe at 8 weeks of age
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• impaired structural and functional cellular differentiation of the prostate epithelium, predominantly in the anterior and dorsolateral lobes
• in anterior and dorsolateral prostate lobes, the epithelium is increased from 38 to 54% and from 32 to 48%, respectively, whereas lumen is decreased from 29 to 18% and 27 to 20%, respectively
• in ventral prostate lobes, stromal volume is unchanged, luminal volume is nonsignificantly reduced but absolute epithelial volume is markedly reduced
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• at 8 weeks of age, all prostate lobes are smaller than normal
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• at 8 weeks of age, the weights of anterior, dorsolateral and ventral lobes are decreased by 64%, 53% and 20%, respectively, relative to control values
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• abnormal multilayered epithelium is present in the seminal vesicles
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• seminal vesicles weigh 45% of that in wild-type mice
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• at 8 weeks of age, significantly reduced epididymal weights despite normal testis weights and normal serum testosterone concentrations in mature males
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• despite a normal macroscopic appearance with normal epithelial branching and normal numbers of ductal tips, all prostate lobes are small by weight
• stroma is reduced and eosinophilic secretion in prostatic lumen is limited
• abnormal clustering of epithelial cells in the anterior lobe and to a lesser extent in the dorsolateral and ventral prostate are present
• increased proliferation of epithelial cells in the anterior prostate
• increased density of basal epithelial cells surrounding the abnormal epithelial cell clusters in the anterior prostate
• absolute volume of each cellular compartment (stroma, lumen, and epithelium) is significantly reduced in the dorsolateral and anterior prostate lobes
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• increased proliferation and abnormal lesions of epithelial cells predominantly in the anterior prostate lobe at 8 weeks of age
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• impaired structural and functional cellular differentiation of the prostate epithelium, predominantly in the anterior and dorsolateral lobes
• in anterior and dorsolateral prostate lobes, the epithelium is increased from 38 to 54% and from 32 to 48%, respectively, whereas lumen is decreased from 29 to 18% and 27 to 20%, respectively
• in ventral prostate lobes, stromal volume is unchanged, luminal volume is nonsignificantly reduced but absolute epithelial volume is markedly reduced
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• at 8 weeks of age, all prostate lobes are smaller than normal
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• at 8 weeks of age, the weights of anterior, dorsolateral and ventral lobes are decreased by 64%, 53% and 20%, respectively, relative to control values
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• abnormal multilayered epithelium is present in the seminal vesicles
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• seminal vesicles weigh 45% of that in wild-type mice
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N |
• at 8 weeks of age, males are overtly healthy with normal physical activity and normal body weights relative to control littermates
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• mutant males display normal testis morphology and sperm production
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• during in vitro fertilization, sperm released from mutant cauda epididymis show an increase in abnormal coiled and bent tails with hairpin structures
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• although the size of lumen is reduced it is still filled with strongly eosinophilic secretions, suggestive of partly preserved epithelial secretory function
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• in the proximal region, the epithelial cells of mutant seminal vesicles appear to be quite low, cuboidal, and with very little cytoplasm, while the epithelial layer is less folded than normal
• in the distal region, the epithelium exhibits a normal, tall secretory morphology, with occasional small foci of hyperplastic epithelial cells and a decrease in the size of the acini
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• the stromal smooth muscle layer appears disorganized and thinner than normal
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• at 8 weeks of age, the size of mutant seminal vesicles is only 55% of that in control littemates
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• at 8 weeks of age, the weight of seminal vesicle secretion and emptied gland is significantly reduced
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• despite normal testis sperm production, sperm numbers are reduced in the caput, normal in the corpus, but significantly increased in the cauda epididymis, suggesting altered sperm epididymal transit kinetics
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• mutant epididymi display stromal thickening in the cauda region
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• mutant epididymi display stromal thickening in the corpus region
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• at 4 hrs after coitus, plugs are detected in equivalent proportions of females mating with control or mutant males; however, plugs formed by mutant males are significantly smaller, lighter, and softer with a fibrous consistency
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• the few mutant males that sired a litter yielded an average of 6.2 1.8 pups/litter relative to 9.6 2.7 pups/litter for control males
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• unlike control males which yielded an average of 3.7 0.4 litters over a 90-day mating period, only 5 of 15 mutant males (33%) had a single litter, and only 1 of 15 had two litters within the same mating period
• median time to the first litter was 21 days and 42 days for second litter for control males, compared with >90 days for both first and second litters for mutant males
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• mutant cauda epididymal sperm show an increased rate of spontaneous acrosome reaction relative to wild-type sperm; hyperactivated, more erratic, and whiplash-like movement typical of capacitated sperm are observed
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• in vivo fertility, as measured by oocytes fertilized by mutant males mated with hyperstimulated females at 6 hrs after copulation, is significantly reduced
• in vitro fertilization capacity of mutant cauda epididymal sperm is significantly reduced at lower sperm concentrations (<50,000 sperm/ml) but not at higher sperm concentrations (>50,000 sperm/ml), indicating a mild defect in sperm binding to the zona pellucida that can be overcome by higher sperm numbers
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• after overnight mating with mutant males, the proportion of plug positive females is significantly reduced, probably due to production of malformed and smaller plugs that are more readily dissolved or dropped
• however, at 4 hrs after coitus, plugs are detected in equivalent proportions of females mated with control or mutant males, indicating successful mating
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• although the size of lumen is reduced it is still filled with strongly eosinophilic secretions, suggestive of partly preserved epithelial secretory function
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• in the proximal region, the epithelial cells of mutant seminal vesicles appear to be quite low, cuboidal, and with very little cytoplasm, while the epithelial layer is less folded than normal
• in the distal region, the epithelium exhibits a normal, tall secretory morphology, with occasional small foci of hyperplastic epithelial cells and a decrease in the size of the acini
|
|
|
• the stromal smooth muscle layer appears disorganized and thinner than normal
|
|
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• at 8 weeks of age, the size of mutant seminal vesicles is only 55% of that in control littemates
|
|
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• at 8 weeks of age, the weight of seminal vesicle secretion and emptied gland is significantly reduced
|
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• during in vitro fertilization, sperm released from mutant cauda epididymis show an increase in abnormal coiled and bent tails with hairpin structures
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
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• seminiferous tubules with smaller diameters are observed
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• testis weights at 3 weeks are significantly lower (15.9 mg) than control males (24.9-27 mg)
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• spermatogenic arrest predominantly at meiotic stage with no elongated spermatids is observed at 9 weeks
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• seminiferous tubules with smaller diameters are observed
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• testis weights at 3 weeks are significantly lower (15.9 mg) than control males (24.9-27 mg)
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
N |
• total body weights of males are equivalent to wild-type males
|
|
|
• tubules with smaller diameters are observed at 9 weeks
• at 9 weeks total Sertoli cell numbers are same as in controls
|
|
|
• at 9 weeks, interstitial Leydig cells display hypertrophy and accumulation of lipid droplets
• adult Leydig cells are present in testes at 9 weeks
|
|
|
• Leydig cells display hypertrophy at 9 weeks
|
|
|
• at 5 or 10 days, testis weights are normal, but testis weights at 3 weeks are significantly lower (15.9 mg) than control males (24.9-27 mg)
|
|
|
• spermatogenic arrest predominantly at meiotic stage with no elongated spermatids is observed at 9 weeks
|
|
|
• tubules with smaller diameters are observed at 9 weeks
• at 9 weeks total Sertoli cell numbers are same as in controls
|
|
|
• at 9 weeks, interstitial Leydig cells display hypertrophy and accumulation of lipid droplets
• adult Leydig cells are present in testes at 9 weeks
|
|
|
• Leydig cells display hypertrophy at 9 weeks
|
|
|
• at 5 or 10 days, testis weights are normal, but testis weights at 3 weeks are significantly lower (15.9 mg) than control males (24.9-27 mg)
|
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
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• hemizygous floxed males are normal in size and display normal fertility and sexual differentiation
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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