Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Irf4tm1.1Rdf mutation
(0 available);
any
Irf4 mutation
(27 available)
|
|
|
immune system
|
|
• mice have decreased percentages of IgD+, CD23+, and PNA+ B cells compared to controls
|
|
|
• complete absence of germinal centers in spleen
|
|
|
• in culture, B cells treated with LPS to induce plasmablastic differentiation do not differentiate, whereas wild-type B cells do
|
|
|
• rate is slower than wild-type cells
|
|
|
• percentage of cells expressing IgG1 after stimulation with CD40 plus Il-4 is much lower than in wild-type cultures after same number of cell divisions
|
hematopoietic system
|
|
• mice have decreased percentages of IgD+, CD23+, and PNA+ B cells compared to controls
|
|
|
• complete absence of germinal centers in spleen
|
|
|
• in culture, B cells treated with LPS to induce plasmablastic differentiation do not differentiate, whereas wild-type B cells do
|
|
|
• rate is slower than wild-type cells
|
|
|
• percentage of cells expressing IgG1 after stimulation with CD40 plus Il-4 is much lower than in wild-type cultures after same number of cell divisions
|
cellular
|
|
• rate is slower than wild-type cells
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Irf4tm1.1Rdf mutation
(0 available);
any
Irf4 mutation
(27 available)
Irf4tm1Rdf mutation
(1 available);
any
Irf4 mutation
(27 available)
|
|
|
immune system
|
N |
• following immunization with a T-dependent antigen, mice show normal percentages of splenic IgD+. CD23+, and PNA+ B cells, germinal centers, and IgG1 and IgM plasma cells, similar to heterozygous Irf4tm1.1Pth mice or doubly heterozygous Irf4tm1Pth / Irf4tm1.1Pth mice
• splenic and peripheral blood B cells show normal T-dependent B cell responses NP hapten, and V(H) rearrangements show similar frequencies of somatic hypermutation
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Irf4tm1.1Rdf mutation
(0 available);
any
Irf4 mutation
(27 available)
Irf4tm1Rdf mutation
(1 available);
any
Irf4 mutation
(27 available)
|
|
|
immune system
|
|
• mature B cells treated with TAT-cre to achieve in vitro deletion of Irf4 are unable to differentiate into plasma cells after LPS treatment, suggesting that it is not a consequence of the developmental stage of the B cells in mutants vs wild-type
|
hematopoietic system
|
|
• mature B cells treated with TAT-cre to achieve in vitro deletion of Irf4 are unable to differentiate into plasma cells after LPS treatment, suggesting that it is not a consequence of the developmental stage of the B cells in mutants vs wild-type
|
immune system
|
N |
• mice form germinal centers and have similar numbers of PNAhi, B220+ B cells in spleen
|
|
|
• following CD40 and Il-4 stimulation, mice have smaller fraction of class-switched cells than control mice (7.4% vs 26.3%); defect appears to be downstream of Cgamma1 germline transcription
|
|
|
• 14 days after immunization with sheep red blood cells, mutants are observed to lack plasma cells, as recognized by CD138 expression, in spleen, peripheral blood and bone marrow
|
|
|
• memory B cell generation occurs in mutants after immunization with NP-KLH, but the population is maintained only partly over time; numbers of NP-binding cells are much lower 42 days after immunization compared to controls
• mice receiving a secondary immunization with antigen lack plasmablasts in the spleen that bind NP, are CD138 +ve and have low B220 expression, indicating that memory B cells cannot differentiate into plasma B cells upon antigen restimulation; B cells also lack IgG1 secretion after restimulation
|
|
|
• mice have much lower titer of IgG1 specific for the hapten NP (nitrophenylacetyl) than controls after immunization with NP coupled to keyhole limpet hemocyanin, but titers of other immunoglobulin classes are similar to levels in controls
|
hematopoietic system
|
|
• following CD40 and Il-4 stimulation, mice have smaller fraction of class-switched cells than control mice (7.4% vs 26.3%); defect appears to be downstream of Cgamma1 germline transcription
|
|
|
• 14 days after immunization with sheep red blood cells, mutants are observed to lack plasma cells, as recognized by CD138 expression, in spleen, peripheral blood and bone marrow
|
|
|
• memory B cell generation occurs in mutants after immunization with NP-KLH, but the population is maintained only partly over time; numbers of NP-binding cells are much lower 42 days after immunization compared to controls
• mice receiving a secondary immunization with antigen lack plasmablasts in the spleen that bind NP, are CD138 +ve and have low B220 expression, indicating that memory B cells cannot differentiate into plasma B cells upon antigen restimulation; B cells also lack IgG1 secretion after restimulation
|
|
|
• mice have much lower titer of IgG1 specific for the hapten NP (nitrophenylacetyl) than controls after immunization with NP coupled to keyhole limpet hemocyanin, but titers of other immunoglobulin classes are similar to levels in controls
|
Analysis Tools