Phenotypes associated with this allele

• Allele Symbol: Plin1^tm1Asg
• Allele Name: targeted mutation 1, Andrew S Greenberg
• Allele ID: MGI:3710993
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plin1^tm1Asg/Plin1^tm1Asg	B6.129S6-Plin1^tm1Asg	MGI:3713712
cx2	Plin1^tm1Asg/Plin1^tm1Asg Tg(Fabp4-Plin*)1Asg/0	involves: 129S6/SvEvTac * C57BL/6	MGI:3713713

Genotype
MGI:3713712

hm1

• Allelic Composition: Plin1^tm1Asg/Plin1^tm1Asg
• Genetic Background: B6.129S6-Plin1^tm1Asg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

adipose tissue phenotype ( J:121641 )

N • interscapular BAT thermal profile is normal; temperature increases ~3 degrees Celsius with norepinephrine infusion

abnormal brown adipose tissue morphology ( J:121641 )

• interscapular BAT depots are darker, display smaller lipid droplets, and have 47% lower triacylglycerol content than wild-type mice

decreased brown adipose tissue amount ( J:121641 )

• interscapular BAT depot weight is reduced relative to controls (0.10 g vs 0.15 g in controls)

decreased white adipose tissue mass ( J:121641 )

• white adipose tissue (WAT) depots are smaller than those in controls

abnormal brown adipose tissue physiology ( J:121641 )

• isolated brown adipocytes show increased basal glycerol and fatty acid release rate compared to Plin-null Tg(Fabp4-Plin*)1Asg adipocytes and wild-type adipocytes; norepinephrine does not produce an increase like that seen in stimulated wild-type adipocytes

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:121641 )

N • norepinephrine-induced thermal response is similar to that of wild-type mice; core temperature increases ~2 degrees Celsius with norepinephrine infusion

abnormal circulating free fatty acids level ( J:113735 )

• forskolin-stimulated lipolysis in Plin-null MEFs transfected with adenovirus expressing Plin is increased ~12-fold compared to basal level of lipolysis in null MEFs, whereas forskolin-stimulated lipolysis is in MEFs expressing Plin with mutated phosphokinase A site is increased only to level of lipolysis exhibited by null MEFs transfected with adenoviral GFP vector

enhanced lipolysis ( J:113735 )

Genotype
MGI:3713713

cx2

• Allelic Composition: Plin1^tm1Asg/Plin1^tm1Asg; Tg(Fabp4-Plin*)1Asg/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

adipose tissue

decreased brown adipose tissue amount ( J:121641 )

• interscapular BAT depots are similar in weight to wild-type mice (0.16 g vs 0.15 g in controls)

• interscapular BAT depots are slightly larger, contain larger lipid droplets, and have 67% less triacylglycerol content than wild-type

abnormal white adipose tissue mass ( J:121641 )

• white adipose tissue depots are smaller than those in controls, but larger than those in Plin-null mice

abnormal adipose tissue physiology ( J:113735 )

• glycerol release in response to norepinephrine treatment is blunted in null MEFs expressing the transgene relative to wild-type MEFs

abnormal brown adipose tissue physiology ( J:121641 )

• interscapular BAT thermal profile is ~70% reduced compared to wild-type; temperature increases >1 degree Celsius

• in brown adipocytes, norepinephrine-induced lipolysis is abrogated relative to wild-type; basal glycerol and fatty acid release are lower than in wild-type or Plin-null adipocytes

• norepinephrine does not stimulate release as it does in wild-type adipocytes

homeostasis/metabolism

abnormal body temperature homeostasis ( J:121641 )

• in response to 30 minutes of norepinephrine infusion, thermal response is significantly blunted compared to wild-type or Plin-null mice; core temperature does not increase significantly with norepineprine infusion

impaired lipolysis ( J:113735 )