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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tcf12tm2Zhu
targeted mutation 2, Yuan Zhuang
MGI:3707160
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Tcf12tm2Zhu/Tcf12tm2Zhu Not Specified MGI:3709887
ht2
Tcf12tm2Zhu/Tcf12+ Not Specified MGI:3709888
ht3
Tcf12tm1Zhu/Tcf12tm2Zhu involves: 129S7/SvEvBrd MGI:3709889
cx4
Tcf12tm2Zhu/Tcf12tm2Zhu
Tg(TcrAND)53Hed/0
involves: C57BL/6 * SJL MGI:3709891


Genotype
MGI:3709887
hm1
Allelic
Composition
Tcf12tm2Zhu/Tcf12tm2Zhu
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm2Zhu mutation (0 available); any Tcf12 mutation (74 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygotes often die within 2 weeks of birth

growth/size/body
• neonates show severe growth retardation

immune system
• fetal thymus shows ~15-fold reduction in cellularity
• thymocyte number is reduced 10-fold relative to Tcf12tm1Zhu homozygotes and 100-fold relative to controls in 2-3 week old mice
• B cell development is impaired
• numbers of immature B cells are reduced compared to controls, but no abnormalities are observed in the peripheral lymph organs
• numbers of pro-b cells are reduced compared to controls, but no abnormalities are observed in the peripheral lymph organs
• T cell development is severely disrupted in peripheral and central lymph organs
• developmental defects in T cells are specific to the alpha/beta lineage, as gamma/delta T cells are detected, although at a reduced number relative to controls
• cells at the double negative 3 (DN3) stage in mutants show severe impairments of V to DJ rearrangements at the TCRbeta locus, in contrast to Tcf12tm1Zhu homozygotes or control wild-type
• fetal thymus shows an accumulation of double negative (DN) T cells compared to controls
• double positive (DP) T cells are nearly absent in the fetal thymus
• defects in T cell development are also seen in the postnatal thymus
• a much tighter and earlier block in development is seen compared to Tcf12tm1Zhu homozygotes; T cell development is almost completely blocked at the double negative stage
• numbers of pre-b cells are reduced compared to controls, but no abnormalities are observed in the peripheral lymph organs
• single positive (SP) T cells are nearly absent in the fetal thymus
• bone marrow cells from neonatal animals transferred to irradiated hosts are able to protect hosts from lethal irradiation, produce myeloid cells, and produce B cells at a reduced efficiency, but T cell development of transferred cells is severely impaired

hematopoietic system
• fetal thymus shows ~15-fold reduction in cellularity
• thymocyte number is reduced 10-fold relative to Tcf12tm1Zhu homozygotes and 100-fold relative to controls in 2-3 week old mice
• B cell development is impaired
• numbers of immature B cells are reduced compared to controls, but no abnormalities are observed in the peripheral lymph organs
• numbers of pro-b cells are reduced compared to controls, but no abnormalities are observed in the peripheral lymph organs
• T cell development is severely disrupted in peripheral and central lymph organs
• developmental defects in T cells are specific to the alpha/beta lineage, as gamma/delta T cells are detected, although at a reduced number relative to controls
• cells at the double negative 3 (DN3) stage in mutants show severe impairments of V to DJ rearrangements at the TCRbeta locus, in contrast to Tcf12tm1Zhu homozygotes or control wild-type
• fetal thymus shows an accumulation of double negative (DN) T cells compared to controls
• double positive (DP) T cells are nearly absent in the fetal thymus
• defects in T cell development are also seen in the postnatal thymus
• a much tighter and earlier block in development is seen compared to Tcf12tm1Zhu homozygotes; T cell development is almost completely blocked at the double negative stage
• numbers of pre-b cells are reduced compared to controls, but no abnormalities are observed in the peripheral lymph organs
• single positive (SP) T cells are nearly absent in the fetal thymus

nervous system
• some homozygous fetuses display exencephaly

endocrine/exocrine glands
• fetal thymus shows ~15-fold reduction in cellularity
• thymocyte number is reduced 10-fold relative to Tcf12tm1Zhu homozygotes and 100-fold relative to controls in 2-3 week old mice




Genotype
MGI:3709888
ht2
Allelic
Composition
Tcf12tm2Zhu/Tcf12+
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm2Zhu mutation (0 available); any Tcf12 mutation (74 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• at 6 to 12 months of age, ~10-20% of mice show seizure episodes upon handling by the tail

hematopoietic system
N
• erythroid and myeloid cell lineages appear normal in heterozygotes

nervous system
• at 6 to 12 months of age, ~10-20% of mice show seizure episodes upon handling by the tail




Genotype
MGI:3709889
ht3
Allelic
Composition
Tcf12tm1Zhu/Tcf12tm2Zhu
Genetic
Background
involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm1Zhu mutation (0 available); any Tcf12 mutation (74 available)
Tcf12tm2Zhu mutation (0 available); any Tcf12 mutation (74 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• T cell development can proceed to the ISP stage but not to the single positive (SP) stage

immune system
• T cell development can proceed to the ISP stage but not to the single positive (SP) stage




Genotype
MGI:3709891
cx4
Allelic
Composition
Tcf12tm2Zhu/Tcf12tm2Zhu
Tg(TcrAND)53Hed/0
Genetic
Background
involves: C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf12tm2Zhu mutation (0 available); any Tcf12 mutation (74 available)
Tg(TcrAND)53Hed mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• block in T cell development is not rescued by transgene expression
• increased ratio of CD4 SP to DP cells is detected in thymus
• no increase in the double positive T cell population or thymic cellularity relative to Tcf12tm2Zhu homozygotes
• an increase in CD4 single positive cells is seen, indicating transgene expression can drive differentiation from the double positive (DP) to single positive (SP) stage
• number of CD4 SP T cells is increased relative to nontransgenic Tcf12-null mice
• numbers of CD4 SP cells in thymus and peripheral lymphoid organs remains small relative to controls

hematopoietic system
• block in T cell development is not rescued by transgene expression
• increased ratio of CD4 SP to DP cells is detected in thymus
• no increase in the double positive T cell population or thymic cellularity relative to Tcf12tm2Zhu homozygotes
• an increase in CD4 single positive cells is seen, indicating transgene expression can drive differentiation from the double positive (DP) to single positive (SP) stage
• number of CD4 SP T cells is increased relative to nontransgenic Tcf12-null mice
• numbers of CD4 SP cells in thymus and peripheral lymphoid organs remains small relative to controls





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last database update
04/30/2024
MGI 6.23
The Jackson Laboratory