Phenotypes associated with this allele

• Allele Symbol: Gfi1^tm4Tmo
• Allele Name: targeted mutation 4, Tarik Moroy
• Allele ID: MGI:3695404
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gfi1^tm4Tmo/Gfi1^tm4Tmo	involves: 129S1/Sv * 129X1/SvJ	MGI:3696978

Genotype
MGI:3696978

hm1

• Allelic Composition: Gfi1^tm4Tmo/Gfi1^tm4Tmo
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal lymphopoiesis ( J:116870 )

abnormal T cell differentiation ( J:116870 )

• homozygotes display impaired development of pre-T-cells, similar to Gfi1^tm1Tmo homozygotes

decreased double-negative T cell number ( J:116870 )

• homozygotes show a significantly reduced frequency (25%) of uncommitted Kit-positive T-cell progenitors i.e. CD4^-/CD8^- pre-T-cells relative to wild-type mice (67%), similar to Gfi1^tm1Tmo homozygotes

abnormal neutrophil morphology ( J:116870 )

• a small % of nearly mature appearing neutrophilic granulocytes with subtle nuclear abnormalities (abortive segmentation, plump segments) is observed

decreased neutrophil cell number ( J:116870 )

• homozygotes are severely neutropenic, similar to Gfi1^tm1Tmo homozygotes

• almost no mature neutrophilic granulocytes are detected in the blood and bone marrow

decreased lymphocyte cell number ( J:116870 )

• the proportion of lymphocytes is reduced in both blood and bone marrow to the same degree as in Gfi1^tm1Tmo homozygotes

decreased thymocyte number ( J:116870 )

• homozygotes display a significant loss of thymic cellularity, similar to Gfi1^tm1Tmo homozygotes

abnormal monocyte morphology ( J:116870 )

• homozygotes show a pronounced increase in monocytoid cells in all tissues examined, similar to Gfi1^tm1Tmo homozygotes

• such atypical monocytoid cells display a greyish-blue, often vacuolated cytoplasm and an indented, bean-shaped or lobed nucleus, but also resemble metamyelocytes, frequently exhibiting a nuclear hole

immune system

abnormal lymphopoiesis ( J:116870 )

abnormal T cell differentiation ( J:116870 )

• homozygotes display impaired development of pre-T-cells, similar to Gfi1^tm1Tmo homozygotes

decreased double-negative T cell number ( J:116870 )

• homozygotes show a significantly reduced frequency (25%) of uncommitted Kit-positive T-cell progenitors i.e. CD4^-/CD8^- pre-T-cells relative to wild-type mice (67%), similar to Gfi1^tm1Tmo homozygotes

abnormal neutrophil morphology ( J:116870 )

• a small % of nearly mature appearing neutrophilic granulocytes with subtle nuclear abnormalities (abortive segmentation, plump segments) is observed

decreased neutrophil cell number ( J:116870 )

• homozygotes are severely neutropenic, similar to Gfi1^tm1Tmo homozygotes

• almost no mature neutrophilic granulocytes are detected in the blood and bone marrow

decreased lymphocyte cell number ( J:116870 )

• the proportion of lymphocytes is reduced in both blood and bone marrow to the same degree as in Gfi1^tm1Tmo homozygotes

decreased thymocyte number ( J:116870 )

• homozygotes display a significant loss of thymic cellularity, similar to Gfi1^tm1Tmo homozygotes

abnormal monocyte morphology ( J:116870 )

• homozygotes show a pronounced increase in monocytoid cells in all tissues examined, similar to Gfi1^tm1Tmo homozygotes

• such atypical monocytoid cells display a greyish-blue, often vacuolated cytoplasm and an indented, bean-shaped or lobed nucleus, but also resemble metamyelocytes, frequently exhibiting a nuclear hole

behavior/neurological

absent pinna reflex ( J:116870 )

• homozygotes fail to show a Preyer reflex

hearing/vestibular/ear

abnormal cochlear inner hair cell morphology ( J:116870 )

• at P0, homozygotes exhibit a single row of morphologically immature IHCs, similar to Gfi1^tm1Tmo homozygotes

abnormal cochlear outer hair cell morphology ( J:116870 )

• at P0, OHCs appear smaller and disorganized

absent outer hair cell stereocilia ( J:116870 )

• at P0, OHC stereocilia are barely identifiable

cochlear outer hair cell degeneration ( J:116870 )

• at P0, homozygotes display progressive OHC degeneration, similar to Gfi1^tm1Tmo homozygotes

deafness ( J:116870 )

• homozygotes display hearing loss, similar to Gfi1^tm1Tmo homozygotes

abnormal vestibular system physiology ( J:116870 )

nervous system

abnormal cochlear inner hair cell morphology ( J:116870 )

• at P0, homozygotes exhibit a single row of morphologically immature IHCs, similar to Gfi1^tm1Tmo homozygotes

abnormal cochlear outer hair cell morphology ( J:116870 )

• at P0, OHCs appear smaller and disorganized

absent outer hair cell stereocilia ( J:116870 )

• at P0, OHC stereocilia are barely identifiable

cochlear outer hair cell degeneration ( J:116870 )

• at P0, homozygotes display progressive OHC degeneration, similar to Gfi1^tm1Tmo homozygotes

endocrine/exocrine glands

decreased thymocyte number ( J:116870 )

• homozygotes display a significant loss of thymic cellularity, similar to Gfi1^tm1Tmo homozygotes