Phenotypes associated with this allele

• Allele Symbol: Trpa1^tm1Jul
• Allele Name: targeted mutation 1, David Julius
• Allele ID: MGI:3692770
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trpa1^tm1Jul/Trpa1^tm1Jul	involves: 129P2/OlaHsd * C57BL/6	MGI:3696956
ht2	Trpa1^tm1Jul/Trpa1^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3696957

Genotype
MGI:3696956

hm1

• Allelic Composition: Trpa1^tm1Jul/Trpa1^tm1Jul
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal nervous system physiology ( J:115886 )

• 10% of cultured trigeminal neurons from P0 mice are responsive to bath-applied mustard oil (MO) or allicin compared to 25% of wild-type neurons; responses are smaller or develop more slowly than in wild-type

Genotype
MGI:3696957

ht2

• Allelic Composition: Trpa1^tm1Jul/Trpa1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

behavior/neurological

increased chemical nociceptive threshold ( J:115886 )

• topical application of mustard oil to the hindpaw produces no acute responses like licking compared to robust responses in wild-type mice

abnormal mechanical nociception ( J:115886 )

• mutants do not display decrease in mechanical threshold (hypersensitivity) after mustard oil treatment like wild-type

hyperalgesia ( J:115886 )

• bradykinin-evoked hyperalgesia (decreased paw withdrawal latency from heat source) is not observed in mutant animals

abnormal thermal nociception ( J:115886 )

• mutants do not display decrease in thermal threshold (hypersensitivity) after mustard oil treatment like wild-type

nervous system

abnormal nervous system physiology ( J:115886 )

• cultured trigeminal neurons from P0 mice show no response to bath-applied mustard oil (MO) or allicin compared to 25% of wild-type neurons (as determined by live cell calcium imaging)

• cultured neurons from the dorsal root ganglia are unresponsive to MO or allicin, compared to 24% of wild-type neurons

• acrolein-evoked responses are absent in cultured mutant trigeminal neurons, whereas acrolein and pentenal evoke large increases in calcium in MO-sensitive neurons

• in response to bradykinin, cultured mutant trigeminal neurons show significantly attenuated responses compared to wild-type neurons (determined by calcium influx)

immune system

abnormal acute inflammation ( J:115886 )

• mustard oil-evoked swelling of treated paw is reduced compared to controls (7% vs 22% increase in thickness)