Phenotypes associated with this allele

• Allele Symbol: Cd79a^tm6Cgn
• Allele Name: targeted mutation 6, University of Cologne
• Allele ID: MGI:3687009
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd79a^tm6Cgn/Cd79a^tm6Cgn	involves: C57BL/6	MGI:3687732

Genotype
MGI:3687732

hm1

• Allelic Composition: Cd79a^tm6Cgn/Cd79a^tm6Cgn
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell cycle ( J:113407 )

• a reduced fraction of B cells are found in S/G₂/M phases 36 and 40 hours after stimulation

abnormal mitosis ( J:113407 )

• after stimulation in culture, B cells divide, but lag behind by ~ 1 cell division compared to controls

decreased B cell proliferation ( J:113407 )

• after stimulation with anti-IgM, B cells are reduced in number

immune system

immune system phenotype ( J:113407 )

N • antigen presentation and T-dependent antibody responses are not different from wild-type responses

N • cap formation and B cell receptor internalization are normal

abnormal B cell number ( J:113407 )

increased transitional stage B cell number ( J:113407 )

• splenic transitional (AA4.1+) B cell numbers are increased by ~40%

decreased B-1a cell number ( J:113407 )

• numbers of splenic B1a B cells are strongly reduced compared to wild-type at 10-12 weeks of age; peritoneal lavage contains ~20% of numbers seen in wild-type mice

abnormal B cell physiology ( J:113407 )

• in vitro, B cells cultured with anti-IgM show defective activation based on CD69 expression and blast formation

• recovery of live cells after 72 hours of culture with anti-IgM is ~10% of wild-type controls

• anti-IgM stimulated B cells show an abnormal tyrosine phophorylation pattern, including some hyperphosphorylated substrates at early time points, compared to wild-type; JNK and ERK phosphorylation are decreased by 30-50% compared to controls

decreased B cell proliferation ( J:113407 )

• after stimulation with anti-IgM, B cells are reduced in number

abnormal immunoglobulin level ( J:113407 )

• NP-specific serum IgG₃ titers are ~25% that of controls after 7, 14, and 21 days; differences disappear at higher doses of NP-Ficoll

• in 8-week old unimmunized mice, titers of IgM, IgG2a and IgG3 are as low as 50% compared to wild-type

decreased IgG2a level ( J:113407 )

decreased IgG3 level ( J:113407 )

decreased IgM level ( J:113407 )

abnormal humoral immune response ( J:113407 )

• numbers of specific IgM-secreting antibody-forming cells 4 days after NP-Ficoll (typeII T-independent antigen) immunization is reduced to ~30% of wild-type levels

hematopoietic system

abnormal B cell number ( J:113407 )

increased transitional stage B cell number ( J:113407 )

• splenic transitional (AA4.1+) B cell numbers are increased by ~40%

decreased B-1a cell number ( J:113407 )

• numbers of splenic B1a B cells are strongly reduced compared to wild-type at 10-12 weeks of age; peritoneal lavage contains ~20% of numbers seen in wild-type mice

abnormal B cell physiology ( J:113407 )

• in vitro, B cells cultured with anti-IgM show defective activation based on CD69 expression and blast formation

• recovery of live cells after 72 hours of culture with anti-IgM is ~10% of wild-type controls

• anti-IgM stimulated B cells show an abnormal tyrosine phophorylation pattern, including some hyperphosphorylated substrates at early time points, compared to wild-type; JNK and ERK phosphorylation are decreased by 30-50% compared to controls

decreased B cell proliferation ( J:113407 )

• after stimulation with anti-IgM, B cells are reduced in number

abnormal immunoglobulin level ( J:113407 )

• NP-specific serum IgG₃ titers are ~25% that of controls after 7, 14, and 21 days; differences disappear at higher doses of NP-Ficoll

• in 8-week old unimmunized mice, titers of IgM, IgG2a and IgG3 are as low as 50% compared to wild-type

decreased IgG2a level ( J:113407 )

decreased IgG3 level ( J:113407 )

decreased IgM level ( J:113407 )