Phenotypes associated with this allele

• Allele Symbol: Unc5a^tm1Lhck
• Allele Name: targeted mutation 1, Lindsay Hinck
• Allele ID: MGI:3665134
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Unc5a^tm1Lhck/Unc5a^tm1Lhck	involves: 129P2/OlaHsd	MGI:3665451

Genotype
MGI:3665451

hm1

• Allelic Composition: Unc5a^tm1Lhck/Unc5a^tm1Lhck
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased neuron apoptosis ( J:111725 )

• at E12 detection of cleaved caspase-3 in the cervical spine shows a 59% decrease in apoptotic cells

• TUNEL assays show a 73% decrease at E12 and a 45% decrease in apoptotic cells at E14

• at E16 and E18, no differences in apoptosis compared to wild-type were detected; no difference is observed at E12 and E16 in the brainstem of mutant and wild-type mice

abnormal spinal cord morphology ( J:111725 )

• by E14, the marginal layer of the cervical spinal cord is consistently smaller and abnormally shaped in mutants

• the width of the marginal layer at this level is significantly reduced compared to wild-type although at E12 and E14 the total area of the spinal cord does not differ from controls

increased motor neuron number ( J:111725 )

• in the cervical spinal cord at E14, the population of motoneurons lying in between the ventral lateral and dorsal medial neuronal pools is 5-fold greater than in wild-type: this region houses spinal accessory motoneurons (SACMNs)

• The ventral lateral pool shows a significantly higher number of neurons as well

cellular

decreased neuron apoptosis ( J:111725 )

• at E12 detection of cleaved caspase-3 in the cervical spine shows a 59% decrease in apoptotic cells

• TUNEL assays show a 73% decrease at E12 and a 45% decrease in apoptotic cells at E14

• at E16 and E18, no differences in apoptosis compared to wild-type were detected; no difference is observed at E12 and E16 in the brainstem of mutant and wild-type mice